Structure, distribution, and biological activity of novel members of the allatostatin family in the crayfish Orconectes limosus

Dircksen, Heinrich; Skiebe, Petra; Abel, Britta; Agricola, Hans‐Jürgen; Buchner, Klaus; Muren, J. Eric; Nässel, Dick R.

doi:10.1016/s0196-9781(99)00052-2

Cited by 88 publications

(85 citation statements)

References 69 publications

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“…The modulatory effects of C-AST in the lobster cardiac system clearly differ in detail from these effects, as they involve changes in the cycle frequency and duration of action potential bursts in pattern-generating neurons of the lobster CG, but not changes at the muscle or neuromuscular junction. Inhibitory effects of all three AST types (A-, B-and C-types) have been documented in the pyloric pattern generator of the stomatogastric system, but details of the mechanisms used to cause the observed decreases in cycle frequency have not yet been examined Dircksen et al, 1999;Fu et al, 2007;Ma et al, 2009;Szabo et al, 2011). Additionally, inhibitory effects of A-type allatostatins have been recorded in the crab CG (Cruz-Bermudez and Marder, 2007).…”

Section: Discussionmentioning

confidence: 99%

Inter-animal variability in the effects of C-type allatostatin on the cardiac neuromuscular system in the lobster Homarus americanus

Wiwatpanit

Powers

Dickinson

2012

Journal of Experimental Biology

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SUMMARYAlthough the global effects of many modulators on pattern generators are relatively consistent among preparations, modulators can induce different alterations in different preparations. We examined the mechanisms that underlie such variability in the modulatory effects of the peptide C-type allatostatin (C-AST; pQIRYHQCYFNPISCF) on the cardiac neuromuscular system of the lobster Homarus americanus. Perfusion of C-AST through the semi-intact heart consistently decreased the frequency of ongoing contractions. However, the effect of C-AST on contraction amplitude varied between preparations, decreasing in some preparations and increasing in others. To investigate this variable effect, we examined the effects of C-AST both peripherally and centrally. When contractions of the myocardium were elicited by controlled stimuli, C-AST did not alter heart contraction at the periphery (myocardium or neuromuscular junction) in any hearts. However, when applied either to the semi-intact heart or to the cardiac ganglion (CG) isolated from hearts that responded to C-AST with increased contraction force, C-AST increased both motor neuron burst duration and the number of spikes per burst by about 25%. In contrast, CG output was increased only marginally in hearts that responded to C-AST with a decrease in contraction amplitude, suggesting that the decrease in amplitude in those preparations resulted from decreased peripheral facilitation. Our data suggest that the differential effects of a single peptide on the cardiac neuromuscular system are due solely to differential effects of the peptide on the pattern generator; the extent to which the peptide induces increased burst duration is crucial in determining its overall effect on the system.

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Section: Discussionmentioning

confidence: 99%

Inter-animal variability in the effects of C-type allatostatin on the cardiac neuromuscular system in the lobster Homarus americanus

Wiwatpanit

Powers

Dickinson

2012

Journal of Experimental Biology

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“…cockroaches, crickets and termites (Stay and Tobe, 2007). A-type peptides have also been found in a number of crustaceans (Duve et al, 1997;Dircksen et al, 1999;Duve et al, 2002;Huybrechts et al, 2003;Fu et al, 2005;Yasuda-Kamatani and Yasuda, 2006;Yin et al, 2006;Christie et al, 2008a;Ma et al, 2008;Gard et al, 2009;Ma et al, 2009), where they have been shown to function as locally released and/or hormonally delivered neuro/myomodulators (Skiebe and Schneider, 1994;Jorge-Rivera and Marder, 1997;Jorge-Rivera et al, 1998;Dircksen et al, 1999;Kreissl et al, 1999;Birmingham et al, 2003;Billimoria et al, 2006;Cruz-Bermúdez and Marder, 2007). Molecular, biochemical and mass spectral analyses have shown that multiple isoforms of A-type peptides are common in both insect and crustacean species, with multiple peptides encoded by the known arthropod A-AST prepro-hormones (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular, biochemical and mass spectral analyses have shown that multiple isoforms of A-type peptides are common in both insect and crustacean species, with multiple peptides encoded by the known arthropod A-AST prepro-hormones (e.g. Duve et al, 1997;Dircksen et al, 1999;Lenz et al, 2000;Meyering-Vos et al, 2001;Duve et al, 2002;Huybrechts et al, 2003;Abdel-Latief et al, 2004;Fu et al, 2005;Yasuda-Kamatani and Yasuda, 2006;Yin et al, 2006;Bowser and Tobe, 2007;Christie et al, 2008a;Ma et al, 2008;Gard et al, 2009;Ma et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification of SYWKQCAFNAVSCFamide: a broadly conserved crustacean C-type allatostatin-like peptide with both neuromodulatory and cardioactive properties

Dickinson

Wiwatpanit

Gabranski

et al. 2009

Journal of Experimental Biology

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SUMMARYThe allatostatins comprise three structurally distinct peptide families that regulate juvenile hormone production by the insect corpora allata. A-type family members contain the C-terminal motif -YXFGLamide and have been found in species from numerous arthropod taxa. Members of the B-type family exhibit a -WX 6 Wamide C-terminus and, like the A-type peptides, appear to be broadly conserved within the Arthropoda. By contrast, members of the C-type family, typified by the unblocked C-terminus -PISCF, a pyroglutamine blocked N-terminus, and a disulfide bridge between two internal Cys residues, have only been found in holometabolous insects, i.e. lepidopterans and dipterans. Here, using transcriptomics, we have identified SYWKQCAFNAVSCFamide (disulfide bridging predicted between the two Cys residues), a known honeybee and water flea C-typelike peptide, from the American lobster Homarus americanus (infraorder Astacidea). Using matrix assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS), a mass corresponding to that of SYWKQCAFNAVSCFamide was detected in the H. americanus brain, supporting the existence of this peptide and its theorized structure. Furthermore, SYWKQCAFNAVSCFamide was detected by MALDI-FTMS in neural tissues from five additional astacideans as well as 19 members of four other decapod infraorders (i.e. Achelata, Anomura, Brachyura and Thalassinidea), suggesting that it is a broadly conserved decapod peptide. In H. americanus, SYWKQCAFNAVSCFamide is capable of modulating the output of both the pyloric circuit of the stomatogastric nervous system and the heart. This is the first demonstration of bioactivity for this peptide in any species.

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“…The first members of this group were identified in the cockroach Diploptera punctata based on their activity as inhibitors of juvenile hormone (JH) synthesis by the corpora allata (CA) (Woodhead et al, 1989;Pratt et al, 1989). Since then, orthologous peptides have been reported in other species of Dictyoptera, as well as in Orthoptera (locusts and crickets), Phasmida, Lepidoptera, Diptera, and Crustacea Decapoda (see Bendena et al, 1999;Dircksen et al, 1999;Lorenz et al, 2000;Duve et al, 2002;Bellés and Maestro, 2005).…”

Section: Introductionmentioning

confidence: 99%

Silencing allatostatin expression using double‐stranded RNA targeted to preproallatostatin mRNA in the German cockroach

Maestro

Bellés

2006

Arch Insect Biochem Physiol

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YXFGL-NH(2) family allatostatins (ASTs) were isolated from cockroach brain extracts based on their capacity to inhibit juvenile hormone (JH) biosynthesis in corpora allata (CA) incubated in vitro. Subsequently, the inhibitory activity of synthetic ASTs was demonstrated experimentally, although these peptides were shown to be active as JH inhibitors only in cockroaches, crickets, and termites. Here, we sought to examine whether ASTs are true physiological regulators of JH synthesis. To this end, we used RNA interference methodologies and the cockroach Blattella germanica as a model. Treatments with double-stranded RNA targeting the allatostatin gene in females of B. germanica produced a rapid and long-lasting reduction in mRNA and peptide levels in both brain and midgut during the reproductive cycle. Nevertheless, while brain AST levels were reduced approximately 70-80%, JH synthesis did not increase in any of the age groups tested.

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Structure, distribution, and biological activity of novel members of the allatostatin family in the crayfish Orconectes limosus

Cited by 88 publications

References 69 publications

Inter-animal variability in the effects of C-type allatostatin on the cardiac neuromuscular system in the lobster Homarus americanus

Inter-animal variability in the effects of C-type allatostatin on the cardiac neuromuscular system in the lobster Homarus americanus

Identification of SYWKQCAFNAVSCFamide: a broadly conserved crustacean C-type allatostatin-like peptide with both neuromodulatory and cardioactive properties

Silencing allatostatin expression using double‐stranded RNA targeted to preproallatostatin mRNA in the German cockroach

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