Structure elucidation and antitumor activity of a water soluble polysaccharide from Hemicentrotus pulcherrimus

Jiang, Yan; Shang, Zhipeng; Lv, Xinyue; Du, Miao; Li, Ma; Hou, Gui‐Ge; Chen, Jing; Wang, Chunhua; Zhao, Feng

doi:10.1016/j.carbpol.2022.119718

Cited by 41 publications

(7 citation statements)

References 44 publications

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“…Ginseng polysaccharides have been reported to exhibit protective effects on gastrointestinal epithelial cells in mice, demonstrating utility in the treatment of conditions such as gastric ulcers and colitis through their anti-inflammatory activities [ 27 ]. Moreover, the acidic polysaccharides of red ginseng possess anticancer effects [ 28 ] and can regulate the immune response, including the activation of macrophages [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Effects of Korean-Red-Ginseng-Derived Polysaccharide on Antibiotic-Associated Diarrhea

Min,

Kim,

Yambe

et al. 2024

Polymers

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This study evaluated the ameliorative effects of Korean-red-ginseng-derived polysaccharide (KRG-P) on antibiotic-associated diarrhea (AAD) induced by administering lincomycin in mice. Changes of intestinal barrier proteins, the intestinal microbiome and short-chain fatty acid (SCFA) contents were investigated. Lincomycin was orally administered for 9 days to induce diarrhea; subsequently, 100 mg/kg and 300 mg/kg of KRG-P were administered orally for 12 days. The diarrhea was observed in the AAD group; further KRG-P administration improved the diarrhea. Analysis of changes in the intestinal microbial flora of the mice revealed that the harmful bacterial flora (such as Proteobacteria) were increased in the AAD group, whereas beneficial bacterial flora (such as Firmicutes) were decreased. However, KRG-P administration resulted in decreased Proteobacteria and increased Firmicutes, supporting the improvement of the microbial flora imbalance caused by AAD. Moreover, an analysis of the SCFAs (acetic acid, propionic acid, and butylic acid) in the caecum revealed that SCFAs’ contents in the AAD group were substantially reduced but tended to increase upon KRG-P administration. Based on these results, KRG-P, which is primarily composed of carbohydrates can improve lincomycin-induced diarrhea, likely owing to the recovery of SCFA content by improving the intestinal microbial imbalance and intestinal barrier proteins.

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Section: Introductionmentioning

confidence: 99%

Ameliorative Effects of Korean-Red-Ginseng-Derived Polysaccharide on Antibiotic-Associated Diarrhea

Min,

Kim,

Yambe

et al. 2024

Polymers

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“…Drawing upon the existing literature [ 46 , 47 , 48 ], our deductions ascertain the principal occurrence of the indicated glycan chains within the constitution of CPTM-P1 (refer to Table 2 ). However, due to the constraints imposed by the finite data yielded by NMR, certain glycan chains characterized by lower prevalence could not be definitively resolved.…”

Section: Resultsmentioning

confidence: 99%

“…Although the sugar chains in CPTM-P1 exhibit similarities, future research must investigate the specific linkage patterns within CPTM-P1 and their influence on immunomodulatory activity. Drawing upon the existing literature [46][47][48], our deductions ascertain the principal occurrence of the indicated glycan chains within the constitution of CPTM-P1 (refer to Table 2). However, due to the constraints imposed by the finite data yielded by NMR, certain glycan chains characterized by lower prevalence could not be definitively resolved.…”

Section: Nuclear Magnetic Resonance (Nmr) Analysismentioning

confidence: 99%

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Fan,

Huang,

Dou

et al. 2024

Molecules

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Polysaccharides extracted from Taxus media hrough an aqueous method were further refined by removing proteins via the Sevag technique and purified by dialysis. The separation of these polysaccharides was accomplished using a DEAE-cellulose chromatog-raphy column, yielding two distinct fractions, named CPTM-P1 and CPTM-P2. Notably, CPTM-P1 emerged as the primary polysaccharide component within Taxus media. Consequently, a comprehensive analysis focusing exclusively on CPTM-P1 was undertaken. The molecular weight of CPTM-P1 was established through gel permeation chromatography (GPC), and its monosaccharide composition was deciphered using HPLC-MS. The structure was further elucidated through nuclear magnetic resonance (NMR) spectroscopy. The molecular weight of CPTM-P1 was determined to be 968.7 kDa. The monosaccharide composition consisted of galactose (Gal), arabinose (Ara), galacturonic acid (Gal-UA), glucose (Glc), rhamnose (Rha), xylose (Xyl), mannose (Man), fucose (Fuc), glucuronic acid (Glc-UA), and ribose (Rib). The proportional distribution of these components was 30.53%, 22.00%, 5.63%, 11.67%, 11.93%, 1.69%, 8.50%, 1.23%, 5.63%, and 1.17%, respectively. This confirmed CPTM-P1 as an acidic heteropolysaccharide with a glycuronic acid backbone. Moreover, CPTM-P1 showed immunoenhancing properties, effectively augmenting the secretion of nitric oxide and cytokines (TNF-α, IL-1β, and IL-6). Additionally, it significantly enhances the phagocytic capacity of RAW264.7 cells. These findings underscore the potential application of these polysaccharides in functional foods and pharmaceuticals, providing a solid scientific basis for further exploration and utilization of Taxus media polysaccharides.

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“…In general, polysaccharides are macromolecules with low toxicity ( 156 , 157 ), which can be widely used in the development of various functional foods. Polysaccharides also have a therapeutic effect on HUA by a mechanism related to the inhibition of XOD and ADA activity to reduce UA production.…”

Section: Bioactive Substances and Their Uric Acid-lowering Mechanismmentioning

confidence: 99%

Research progress on the prevention and treatment of hyperuricemia by medicinal and edible plants and its bioactive components

Cheng-yuan,

Jian-gang

2023

Front. Nutr.

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Hyperuricemia is another common metabolic disease, which is considered to be closely related to the development of many chronic diseases, in addition to the “three highs.” Currently, although drugs show positive therapeutic effects, they have been shown to produce side effects that can damage the body. There is growing evidence that medicinal and edible plants and their bioactive components have a significant effect on hyperuricemia. In this paper, we review common medicinal and edible plants with uric acid-lowering effects and summarize the uric acid-lowering mechanisms of different bioactive components. Specifically, the bioactive components are divided into five categories: flavonoids, phenolic acids, alkaloids, polysaccharides, and saponins. These active substances exhibit positive uric acid-lowering effects by inhibiting uric acid production, promoting uric acid excretion, and improving inflammation. Overall, this review examines the potential role of medicinal and edible plants and their bioactive components as a means of combating hyperuricemia, with the hope of providing some reference value for the treatment of hyperuricemia.

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Structure elucidation and antitumor activity of a water soluble polysaccharide from Hemicentrotus pulcherrimus

Cited by 41 publications

References 44 publications

Ameliorative Effects of Korean-Red-Ginseng-Derived Polysaccharide on Antibiotic-Associated Diarrhea

Ameliorative Effects of Korean-Red-Ginseng-Derived Polysaccharide on Antibiotic-Associated Diarrhea

Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media

Research progress on the prevention and treatment of hyperuricemia by medicinal and edible plants and its bioactive components

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