Structure, fluorescence quenching and antioxidant activity of a carbohydrate polymer from Eugenia jambolana

Bandyopadhyay, Shruti S.; Ghosh, Debjani; Micard, Valérie; Sinha, Sharmistha; Chatterjee, Udipta R.; Ray, Bimalendu

doi:10.1016/j.ijbiomac.2012.04.004

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…Notably, the commercial enzyme preparation used in this study was found to possesses endogalactanase activity. 50 Taken together, this carbohydrate polymer possess a structure that has not been reported earlier.…”

Section: ■ Materials and Methodsmentioning

confidence: 53%

“…ESI-mass spectrum (Figure ) of acetylated oligomers (AO) revealed the presence of numerous fragments. Notably, the commercial enzyme preparation used in this study was found to possesses endogalactanase activity . On the basis of their molecular weight, ions at m / z 413, 701, 989, 1205, 1277, 1421, 1565, 1710, 1853, 1998, and 2141, respectively, corresponding to [M + Na] of Hex 1 Ac 5 (Hex 1 = one hexose residue, Ac 5 = five acetyl substituent), Hex 2 Ac 8 , Hex 3 Ac 11 , Hex 3 Pent 1 Ac 13 (Pent 1 = one pentose residue), Hex 4 Ac 14 , Hex 1 Pent 4 CA 1 Ac 13 (CA 1 = one coumaric acid residue), Hex 5 Ac 17 , Hex 2 Pent 4 CA 1 Ac 16 , Hex 6 Ac 20 , Hex 3 Pent 4 CA 1 Ac 19 , and Hex 7 Ac 23 .…”

Section: Resultsmentioning

confidence: 99%

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Antioxidative Carbohydrate Polymer from Enhydra fluctuans and Its Interaction with Bovine Serum Albumin

et al. 2013

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Enhydra fluctuans leaves are traditionally sold on Indian markets for various health benefits. However, no phytochemical study on its high molecular weight compound has so far been performed. Chemical, chromatographic, ESI-TOF-MS, and NMR analyses of the water extracted carbohydrate polymer (CP) of E. fluctuans leaves showed the presence of a 24 kDa arabinogalactan having a (1,3)-linked β-d-Galp main chain, substituted at O-6 by (1,6)-linked β-d-Galp side chains. The latter residues were substituted at O-3 by (1,3)-, (1,5)-, and (1,3,5)-linked α-l-Araf chains, and nonreducing end-units of α-l-Araf and β-d-Galp. This polymer contained esterified phenolic acids. Biochemical analysis revealed similarity in antioxidative potential between the identified carbohydrate polymer and known standard antioxidants. The highly branched side chains and the phenolic acid residues of the arabinogalactan might be the functional sites. Fluorimetric and ultraviolet spectrometric analyses showed that the studied carbohydrate polymer can form complex with bovine serum albumin having binding constant K = 2.42 × 10(6)/M and changes its microenvironment. Thus, traditional aqueous extraction method provides a carbohydrate polymer, which stimulates a fair biological response: this could represent an interesting approach in phytotherapeutic treatments.

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Section: ■ Materials and Methodsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

Antioxidative Carbohydrate Polymer from Enhydra fluctuans and Its Interaction with Bovine Serum Albumin

et al. 2013

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“…Such type of fluorescence quenching can be verified through the Stern–Volmer equation, as shown in Eq. (1) [2] , [3] .

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Binding constants of Southern rice black-streaked dwarf virus Coat Protein with ferulic acid derivatives

et al. 2018

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The data present binding constants between ferulic acid derivatives and the Coat Protein (P10) by fluorescence titration in this article, which is hosted in the research article entitled “Interaction Research on an Antiviral Molecule that Targets the Coat Protein of Southern rice black-streaked dwarf virus’’ (Ran et al., 2017) [1]. The data include fluorescence quenching spectrum, Stern–Volmer quenching constants, and binding parameters. In this article, a more comprehensive data interpretation and analysis is explained.

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“…Jamun possesses diverse health benefits that can be accredited due to the presence of various pharmacological active phytoconstituents such as tannins, alkaloids, steroids, flavanoids, terpenoids, fatty acids and anthocyanins [1, 4]. Seeds of S. cumini are used as a remedy for diabetes mellitus and as a productive source of natural antioxidants [5, 6]. It has various pharmacological activities such as hypoglycaemic, cardioprotective, antioxidant, anti-proliferative, anti-inflammatory, chemoprotective and gastroprotective properties that have been proven on animal models by different researches [7– 13].…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of DNA polymorphisms and phylogenetic relationships among Syzygium cumini Skeels based on phenotypic characters and RAPD technique

Singh

Bajpai

et al. 2014

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The Indian black berry (Syzygium cumini Skeels) has a great nutraceutical and medicinal properties. As in other fruit crops, the fruit characteristics are important attributes for differentiation were also determined for different accessions of S. cumini. The fruit weight, length, breadth, length: breadth ratio, pulp weight, pulp content, seed weight and pulp: seed ratio significantly varied in different accessions. Molecular characterization was carried out using PCR based RAPD technique. Out of 80 RAPD primers, only 18 primers produced stable polymorphisms that were used to examine the phylogenetic relationship. A sum of 207 loci were generated out of which 201 loci found polymorphic. The average genetic dissimilarity was 97 per cent among jamun accessions. The phylogenetic relationship was also determined by principal coordinates analysis (PCoA) that explained 46.95 per cent cumulative variance. The two-dimensional PCoA analysis showed grouping of the different accessions that were plotted into four sub-plots, representing clustering of accessions. The UPGMA (r = 0.967) and NJ (r = 0.987) dendrogram constructed based on the dissimilarity matrix revealed a good degree of fit with the cophenetic correlation value. The dendrogram grouped the accessions into three main clusters according to their eco-geographical regions which given useful insight into their phylogenetic relationships.

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Structure, fluorescence quenching and antioxidant activity of a carbohydrate polymer from Eugenia jambolana

Cited by 8 publications

References 39 publications

Antioxidative Carbohydrate Polymer from Enhydra fluctuans and Its Interaction with Bovine Serum Albumin

Antioxidative Carbohydrate Polymer from Enhydra fluctuans and Its Interaction with Bovine Serum Albumin

Binding constants of Southern rice black-streaked dwarf virus Coat Protein with ferulic acid derivatives

Comparative analysis of DNA polymorphisms and phylogenetic relationships among Syzygium cumini Skeels based on phenotypic characters and RAPD technique

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