Structure–Function Analysis of the Inverted Terminal Repeats of the Sleeping Beauty Transposon

Cui, Zongbin; Geurts, Aron M.; Liu, Geyi; Kaufman, Christopher D.; Hackett, Perry B.

doi:10.1016/s0022-2836(02)00237-1

Cited by 212 publications

(219 citation statements)

References 44 publications

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“…Point mutations which increase transposition activity have been identified either by inference from related transposases or by alanine scanning substitutions (SB11 (Geurts et al, 2003), SB12 (Zayed et al, 2004), HSB1, HSB2, HSB3, HSB4 (Yant et al, 2004)). Increase transposition frequency has also been achieved by specific point mutations (pT2 (Cui et al, 2002)) or by direct replacement of the right IRDR with the left IRDR (pT3 (Yant et al, 2004)). …”

Section: Dna Transposonsmentioning

confidence: 99%

Retroviral insertional mutagenesis: past, present and future

et al. 2005

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Retroviral insertion mutagenesis screens in mice are powerful tools for efficient identification of oncogenic mutations in an in vivo setting. Many oncogenes identified in these screens have also been shown to play a causal role in the development of human cancers. Sequencing and annotation of the mouse genome, along with recent improvements in insertion site cloning has greatly facilitated identification of oncogenic events in retrovirus-induced tumours. In this review, we discuss the features of retroviral insertion mutagenesis screens, covering the mechanisms by which retroviral insertions mutate cellular genes, the practical aspects of insertion site cloning, the identification and analysis of common insertion sites, and finally we address the potential for use of somatic insertional mutagens in the study of nonhaematopoietic and nonmammary tumour types.

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Section: Dna Transposonsmentioning

confidence: 99%

Retroviral insertional mutagenesis: past, present and future

et al. 2005

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“…The resulting hIDUA expression cassette was excised at the SspI and HindIII sites and inserted between the EcoRV and HindIII sites of pT2/BH [30]. A 180-bp spacer sequence, amplified by polymerase chain reaction (PCR) from the 3′UTR of the human β-globin gene, was inserted into the XhoI site between the transposon and the transposase expression cassette.…”

Section: Plasmidsmentioning

confidence: 99%

“…We also updated the SB transposon system (T2) and used SB11 transposase to increase the frequency of transposition [28,30]. To ensure simultaneous delivery of transposon and transposase into cells, we engineered cis-vectors in which both the transposon and a transposase expression cassette were on the same plasmid ( Figure 1A).…”

Section: Correction Of Idua Deficiency In Mps I Micementioning

confidence: 99%

Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon‐mediated gene delivery: implications for non‐viral gene therapy of mucopolysaccharidoses

Aronovich

Bell

Belur

et al. 2007

The Journal of Gene Medicine

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“…However, the activity was low and insufficient for detecting transposition. In the present study, we used a hyperactive variant of the SB transposon (pT2; Cui et al, 2002) and SB transposase (SB100X; Mates et al, 2009). These variants increased both the excision and transposition activity of the SB system in C. intestinalis to achieve germline transformation, suggesting that artificial mutagenesis of transposon/transposase in laboratories is a good strategy to improve transformation systems.…”

Section: Discussionmentioning

confidence: 99%

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element

Hozumi

Mita

Miskey

et al. 2012

Developmental Dynamics

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Background: Transposon-mediated transgenesis is an excellent method for creating stable transgenic lines and insertional mutants. In the chordate Ciona intestinalis, Minos is the only transposon that has been used as the tool for germline transformation. Adding another transposon system in this organism enables us to conduct genetic techniques which can only be realized with the use of two transposons. Results: In the present study, we found that another Tc1/mariner superfamily transposon, sleeping beauty (SB), retains sufficient activity for germline transformation of C. intestinalis. SB shows efficiencies of germline transformation, insertion into gene coding regions, and enhancer detection comparable to those of Minos. We have developed a system for the remobilization of SB copies in the C. intestinalis genome by using transgenic lines expressing SB transposase in the germ cells. With this system, we examined the manner of SB mobilization in the C. intestinalis genome. SB shows intrachromosomal transposition more frequently than Minos. Conclusions: SB-based germline transformation and the establishment of a new method that uses its frequent intrachromosomal transposition will result in breakthroughs in genetic approaches that use C. intestinalis together with Minos. Developmental Dynamics 242:30-43, 2013. V C 2012 Wiley Periodicals, Inc.Key words: ascidian; Ciona intestinalis; transposon; Tc1; mariner; sleeping beauty; Minos Key findings:In C. intestinalis, Minos is the only transposon that has been used as a tool for germline transformation. We found that another transposon, sleeping beauty (SB), retains sufficient activity for the germline transformation of C. intestinalis. SB-based germline transformation will result in breakthroughs in genetic approaches that use C. intestinalis together with Minos.

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Structure–Function Analysis of the Inverted Terminal Repeats of the Sleeping Beauty Transposon

Cited by 212 publications

References 44 publications

Retroviral insertional mutagenesis: past, present and future

Retroviral insertional mutagenesis: past, present and future

Prolonged expression of a lysosomal enzyme in mouse liver after Sleeping Beauty transposon‐mediated gene delivery: implications for non‐viral gene therapy of mucopolysaccharidoses

Germline transgenesis of the chordate Ciona intestinalis with hyperactive variants of sleeping beauty transposable element

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