Structure-Function Relationships of Oxygen Transport Proteins in Marine Invertebrates Enduring Higher Temperatures and Deoxygenation

Coates, Christopher J.; Belato, Flávia Ariany; Halanych, Kenneth M.; Costa-Paiva, Elisa Maria

doi:10.1086/722472

Cited by 3 publications

(4 citation statements)

References 133 publications

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“…[ 20 ] Forming a part of the oxygen transport system, HB is known to play a significant role in various organisms. [ 21 ] In contrast to vertebrates, HB is found sporadically in different invertebrate phyla, [ 22 ] with body fluid concentrations typically lower than those of vertebrates. [ 23 ] HB in D. magna was discovered more than 100 years ago, [ 24 ] and various studies have been conducted.…”

Section: Resultsmentioning

confidence: 99%

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Shin

et al. 2023

J Biochem & Molecular Tox

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Triclosan has been widely used as an antimicrobial agent. However, triclosan was found to cause toxicity, including muscle contraction disturbances, carcinogenesis, and endocrine disorders. In addition, it was found to affect central nervous system function adversely and even have ototoxic effects. Conventional methods for detecting such triclosan can be performed easily. However, the conventional detection methods are inadequate in precisely reflecting the impact of toxic substances on stressed organisms. Therefore, a test model for the toxic environment at the molecular level through the organism is needed. From that point of view, Daphnia magna is being used as a ubiquitous model. D. magna has the advantages of easy cultivation, a short lifespan and high reproductive capacity, and high sensitivity to chemicals. Therefore, the protein expression pattern of D. magna that appear in response to chemicals can be utilized as biomarkers for detecting specific chemicals. In this study, we characterized the proteomic response of D. magna following triclosan exposure via two‐dimensional (2D) gel electrophoresis. As a result, we confirmed that triclosan exposure completely suppressed D. magna 2‐domain hemoglobin protein and evaluated this protein as a biomarker for triclosan detection. We constructed the HeLa cells in which the GFP gene was controlled by D. magna 2‐domain hemoglobin promoter, which under normal conditions, expressed GFP, but upon triclosan exposure, suppressed GFP expression. Consequently, we consider that the HeLa cells containing the pBABE‐HBF3‐GFP plasmid developed in this study can be used as novel biomarkers for triclosan detection.

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Section: Resultsmentioning

confidence: 99%

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Shin

et al. 2023

J Biochem & Molecular Tox

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“…The combined and interactive effects of hypoxia and acidification remain poorly understood, although they share common links to respiration (Gobler and Baumann, 2016). Low environmental oxygen can directly depress oxygen content of blood and tissues, while high CO 2 /low pH in the environment, if directly translated into the interior of the organism, can modulate the oxygen-binding affinity and capacity of oxygen transport proteins (when present) (Coates et al, 2022). Thus, both stressors can elicit compensatory changes in ventilation, circulation, and acid-base status with associated changes in metabolism (Lehtonen and Burnett, 2016).…”

Section: Interactions Between Oxygen and Hypercapnia/acidificationmentioning

confidence: 99%

“…First, oxygen transport proteins demonstrate impressive resistance to thermal denaturation: hemoglobins and hemocyanins remain undissociated at temperatures >50 7C and >90 7C, respectively (e.g., Georgieva et al, 1998;Carvalho et al, 2013). Thus, oxygen transport proteins are likely to remain structurally intact under substantial changes in ocean conditions (see Coates et al, 2022), although their functional robustness in vivo will also depend on temperature effects on other processes. Second, organisms can adaptively increase oxygen transport capacity by increasing the expression of oxygen transport proteins or express isoforms with a different oxygen affinity (Decker and Föll, 2000;Brown-Peterson et al, 2005).…”

Section: Molecular Responses and Oxygen Transport Proteinmentioning

confidence: 99%

“…The papers in this issue highlight, from diverse perspectives, the importance of oxygen interactions, including their effects on the function of oxygen transport proteins (Coates et al, 2022), immune defense (Burnett and Burnett, 2022), organismal performance and survival (Lucey et al, 2022), life-history optimization (Atkinson et al, 2022;Audzijonyte et al, 2022), and plastic and evolutionary responses over multiple timescales (Borges et al, 2022;Earhart et al, 2022;Roman and Pierson, 2022;Sperling et al, 2022;Walczyńska and Sobczyk, 2022). Other factors less often considered, such as light, can also interact with oxygen availability to affect organismal performance in surprising ways (McCormick et al, 2022).…”

Section: Report Measured Conditions More Comprehensivelymentioning

confidence: 99%

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Integrative Approaches to Understanding Organismal Responses to Aquatic Deoxygenation

Woods

Moran

Atkinson

et al. 2022

The Biological Bulletin

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Oxygen bioavailability is declining in aquatic systems worldwide as a result of climate change and other anthropogenic stressors. For aquatic organisms, the consequences are poorly known but are likely to reflect both direct effects of declining oxygen bioavailability and interactions between oxygen and other stressors, including two-warming and acidificationthat have received substantial attention in recent decades and that typically accompany oxygen changes. Drawing on the collected papers in this symposium volume ("An Oxygen Perspective on Climate Change"), we outline the causes and consequences of declining oxygen bioavailability. First, we discuss the scope of natural and predicted anthropogenic changes in aquatic oxygen levels. Although modern organisms are the result of long evolutionary histories during which they were exposed to natural oxygen regimes, anthropogenic change is now exposing them to more extreme conditions and novel combinations of low oxygen with other stressors. Second, we identify behavioral and physiological mechanisms that underlie the interactive effects of oxygen with other stressors, and we assess the range of potential organismal responses to oxygen limitation that occur across levels of biological organization and over multiple timescales. We argue that metabolism and energetics provide a powerful and unifying framework for understanding organism-oxygen interactions. Third, we conclude by outlining a set of approaches for maximizing the effectiveness of future work, including focusing on long-term experiments using biologically realistic variation in experimental factors and taking truly cross-disciplinary and integrative approaches to understanding and predicting future effects.

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A comprehensive review on hemocyanin from marine products: Structure, functions, its implications for the food industry and beyond

Ji,

Guan,

et al. 2024

International Journal of Biological Macromolecules

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Structure-Function Relationships of Oxygen Transport Proteins in Marine Invertebrates Enduring Higher Temperatures and Deoxygenation

Cited by 3 publications

References 133 publications

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Detection of triclosan using 2‐domain hemoglobin promoter of Daphnia magna

Integrative Approaches to Understanding Organismal Responses to Aquatic Deoxygenation

A comprehensive review on hemocyanin from marine products: Structure, functions, its implications for the food industry and beyond

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