Structure of a neutral polymer isolated from the lipopolysaccharide of the reference strain (C.D.C. 4523-60) for Serratia marcescens serogroup O15

Structural and serological studies of lipopolysaccharides from proposed new serotypes (O25 and O26) of Serratia marcescens

“…This result closely parallels that for the 015 antigen [19] and, with components in the inverse ratio, for the 09 antigen [18]. This result closely parallels that for the 015 antigen [19] and, with components in the inverse ratio, for the 09 antigen [18].…”

“…Methylation analysis of the polymeric material (batch B) showed the presence of both 3-substituted Rhap residues (minor) and 4-substituted Rhap residues (major), as well as 3-substituted GalpNAc residues. This result closely parallels that for the 015 antigen [19] and, with components in the inverse ratio, for the 09 antigen [18]. To confirm this resemblance, and to obtain evidence for two separate polymeric fractions in the 026 product, a Smith degradation was carried out.…”

“…Antiserum 026 cross-reacted in ELISA only with 09 and 015, known to possess the same two neutral polysaccharides, albeit in different proportions [18,19]. Like 025, the 026 strain was negative in the Quellung test, but with this strain specific results were seen in ELISA and immunoblots.…”

“…The 'H NMR spectrum of the polymeric product (SDl) contained two anomeric signals (each 1 H) with 64.89 (unresolved) and 4.70 (J,,, 8.5 Hz), and methyl signals with 82.04 (s) and 1.27 cd), clearly corresponding to the periodate-resistant minor component of the original mixture. The parent polymer was the major component of both batches of 026 antigen studied, and is the major component of the 015 antigen [19] and the minor component of the 09 antigen [ 181. 1).…”

“…The unsubstituted GalNAc residue was confirmed by methylation analysis. The parent polymer was the major component of both batches of 026 antigen studied, and is the major component of the 015 antigen [19] and the minor component of the 09 antigen [ 181.…”