Structure of a pectic polysaccharide from Pseudostellaria heterophylla and stimulating insulin secretion of INS-1 cell and distributing in rats by oral

Chen, Jinlong; Pang, Wensheng; Kan, Yongjun; Zhao, Li; He, Zhaodong; Shi, Wentao; Yan, Bing; Chen, Hong; Hu, Juan

doi:10.1016/j.ijbiomac.2017.08.034

Cited by 42 publications

(23 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Hu et al demonstrated the hypoglycemic effect of polysaccharides with different molecular weight and characterized the PF40 fraction effectively prevented the cascade of inflammatory events and blocked overweight progressing to obesity [13]. Chen et al isolated and identified a pectic polysaccharide in the aqueous phase of pseudostellaria heterophylla with potency to stimulate insulin secretion of INS-1 cell [20]. Likewise, here we showed administration of the single-component polysaccharide, H-1-2, via oral gavage significantly enhanced the glucose responsiveness and therefore glucose-stimulated insulin secretion, which was accompanied with decrease of blood glucose, insulin and obvious improvement in respect to the lipid profiles.…”

Section: Discussionmentioning

confidence: 99%

Novel Polysaccharide H-1-2 from Pseudostellaria Heterophylla Alleviates Type 2 Diabetes Mellitus

Fang

Duan

Zhao

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: To investigate the potential therapeutic effect of novel polysaccharide H-1-2 from pseudostellaria heterophylla against type 2 Diabetes Mellitus (T2DM) and elucidate the underling molecular mechanisms. Methods: Relative expression of HIF1α and Sirt1 in T2DM patients was determined via real-time PCR. The direct binding of HIF1α on Sirt1 promoter was validated by ChIP assay. The inhibitory regulation of Sirt1 by HIF1α was analyzed using luciferase reporter assay. The endogenous protein of HIF1α and Sirt1 in response to H-1-2 treatment was quantified by western blotting. The blood glucose, secreted insulin and serous lipid profiles were measured with ELISA kits. Results: We consolidated that HIF1α and Sirt1 was dysregulated in T2DM patients and subjected to H-1-2 modulation. H-1-2 significantly inhibited hypoxia and up-regulated Sirt1 expression in EndoC-βH1 cells. Accordingly, H-1-2 enhanced glucose-stimulation insulin secretion and improved blood glucose and lipid profiles in T2DM cells, and elevated the glucose and insulin tolerance simultaneously. Furthermore, we demonstrated that H-1-2 alleviated T2DM via inhibition of hypoxia and up-regulation of Sirt1 in isolated pancreatic β-cells from T2DM rats. Conclusion: Our data unambiguously demonstrated H-1-2 administration alleviated T2DM by enhancing Sirt1 expression through inhibition of hypoxia.

show abstract

Section: Discussionmentioning

confidence: 99%

Novel Polysaccharide H-1-2 from Pseudostellaria Heterophylla Alleviates Type 2 Diabetes Mellitus

Fang

Duan

Zhao

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Although these promising compounds need to be further experimentally tested against the four analysed targets and confirmed their efficacy or benefit, using computer virtual screening, the number of actual screening compounds can be reduced and the discovery efficiency of lead compounds can be improved. The Chinese medicine compound prescriptions could be considered as promising sources of potential candidates for discovery new drugs 26 .…”

Section: Discussionmentioning

confidence: 99%

Gualou Guizhi decoction reverses brain damage with cerebral ischemic stroke, multi-component directed multi-target to screen calcium-overload inhibitors using combination of molecular docking and protein–protein docking

Pang

Han

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

Self Cite

View full text Add to dashboard Cite

Stroke is a disease of the leading causes of mortality and disability across the world, but the benefits of drugs curative effects look less compelling, intracellular calcium overload is considered to be a key pathologic factor for ischemic stroke. Gualou Guizhi decoction (GLGZD), a classical Chinese medicine compound prescription, it has been used to human clinical therapy of sequela of cerebral ischemia stroke for 10 years. This work investigated the GLGZD improved prescription against intracellular calcium overload could decreased the concentration of [Ca2+]i in cortex and striatum neurone of MCAO rats. GLGZD contains Trichosanthin and various small molecular that they are the potential active ingredients directed against NR2A, NR2B, FKBP12 and Calnodulin target proteins/enzyme have been screened by computer simulation. “Multicomponent systems” is capable to create pharmacological superposition effects. The Chinese medicine compound prescriptions could be considered as promising sources of candidates for discovery new agents.

show abstract

“…Based on the abundant chemical constituents, P. heterophylla has multiple pharmaceutical activities including immunomodulatory [ 3 , 25 ], antidiabetic [ 26 – 29 ], antitussive [ 5 ], antioxidant [ 30 ] activities, as well as protective effects on retinal injury and exercise-induced oxidative stress etc. [ 31 – 33 ].…”

Section: Pharmaceutical Activities Of P Heterophyllamentioning

confidence: 99%

“…However, crude polysaccharides from P. heterophylla are commonly used. A water-soluble, pectic polysaccharide with molecular weight of 48 kDa, composed of rhamnose, galactose, arabinose and galacturonic acid and 1,4-linked galacturonic acid as main chain with small amount of 1,2-linked rhamnose, could obviously stimulated insulin secretion [ 26 ]. A novel homogeneous polysaccharide, named as H-1-2, was also isolated from P. heterophylla polysaccharide.…”

Section: Pharmaceutical Activities Of P Heterophyllamentioning

confidence: 99%

“…However, the biological activities of polysaccharides are closely correlated to their molecular size, types and ratios of constituent monosaccharides, and features of glycosidic linkages (e.g., configuration and position of glycosidic linkages, and sequence of monosaccharides) [ 48 , 49 ]. Recently, HPSEC, HPLC after 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization, NMR, Fourier transform infrared analysis (FT-IR) and chemical method including phenol–sulfuric acid, periodate oxidation, smith hydrolysis, methylation analysis, partial acid hydrolysis, have been applied for evaluating total contents, molecular sizes, types and ratios of constituent monosaccharides, and features of glycosidic linkages of polysaccharides in P. heterophylla [ 22 , 26 , 27 , 50 ].…”

Section: Chemical Analysis Of P Heterophyllamentioning

confidence: 99%

See 1 more Smart Citation

Chemistry, pharmacology and analysis of Pseudostellaria heterophylla: a mini-review

Shakerian

Zhao

et al. 2019

Chin Med

View full text Add to dashboard Cite

Pseudostellaria heterophylla is one of the well-known traditional Chinese medicines and has been used in clinics for 100 years in China. The chemistry and pharmacology of P. heterophylla were reviewed to understand its active compounds. Then analysis of these compounds related to quality control of this herb was discussed. For the analysis of chemicals, three aspects have been discussed in this review. The first two aspects focused on the methodologies for analysis of cyclic peptides and carbohydrates in P. heterophylla, respectively. The last one dealt with the other methods used for identification of P. heterophylla. Some rich chemicals such as oligosaccharides in this plant were rarely evaluated. Many analyses were performed on this plant, however, few of them were accepted as quality control method.

show abstract

Structure of a pectic polysaccharide from Pseudostellaria heterophylla and stimulating insulin secretion of INS-1 cell and distributing in rats by oral

Cited by 42 publications

References 8 publications

Novel Polysaccharide H-1-2 from Pseudostellaria Heterophylla Alleviates Type 2 Diabetes Mellitus

Novel Polysaccharide H-1-2 from Pseudostellaria Heterophylla Alleviates Type 2 Diabetes Mellitus

Gualou Guizhi decoction reverses brain damage with cerebral ischemic stroke, multi-component directed multi-target to screen calcium-overload inhibitors using combination of molecular docking and protein–protein docking

Chemistry, pharmacology and analysis of Pseudostellaria heterophylla: a mini-review

Contact Info

Product

Resources

About