Structure of Adenanthin

Diterpenoids from isodon calcicola var. subculva

“…Calcicolin B (1) was assigned the molecular formula C 24 C and DEPT NMR spectra of 1 were very similar to those of adenanthin (5) [4], a known kaurenoid also isolated from I. calcicola var. subculva, the only dierence being the absence of a signal at d 1.82 and dc 169.2 in the spectra of 1.…”

“…A previous phytochemical investigation [2] has shown the presence of two ent-kaurenoids, calcicolin A and weisiensin A [3], in this plant. The reinvestigation of this plant has led to the isolation of four new diterpenoids, calcicolin B±E (1±5), together with ®ve known diterpenoids, calcicolin A, weisiensin A [2], adenanthin (5) [4], forrestin C (7) [5] and nervosanin [6]. In this paper, we report on the isolation and structure elucidation of these new compounds by spectral analysis.…”

Adenanthin, a Natural ent-Kaurane Diterpenoid Isolated from the Herb Isodon adenantha Inhibits Adipogenesis and the Development of Obesity by Regulation of ROS

“…In summary, adenanthin, a natural ent -kaurane diterpenoid isolated from the herb Isodon adenantha in 1987 [27], was able to inhibit adipogenesis of 3T3-L1 and mouse embryonic fibroblasts (MEFs) through regulation of ROS and significantly reduced the growing body weight and adipose tissue mass during HFD-induced development of obesity in mice, revealing adenanthin may be a potential agent for prevention of obesity.…”

“…Fraction B (250 g), brown gum, was subjected to column chromatography over a silica gel (200–300 mesh) column, eluted with Petroleum ether–Me 2 CO (1:0–0:1) gradient system, to obtain five fractions, B1–B5. Adenanthin (16.5 g) was precipitated from fraction B2, it had a degree of purity >98% [27]. The NMR spectra, MS spectra, and HRESIMS spectra have been shown in supplementary data.…”

“…Adenanthin, an ent -kaurane diterpenoid isolated from the herb Isodon adenantha [27], has been shown to target Prx 1 and Prx 2 to exert its bioactivity in increasing ROS of leukemic cells [28], inhibiting NF- κ B signaling [29] and killing hepatocellular carcinoma cells [30]. However, the effect of adenanthin on adipogenesis and the development of obesity is unknown.…”