Structure of cDNAs Encoding Human Eukaryotic Initiation Factor 3 Subunits

Asano, Katsura; Vornlocher, Hans Peter; Richter-Cook, Nancy J.; Merrick, William C.; Hinnebusch, Alan G.; Hershey, John W.B.

doi:10.1074/jbc.272.43.27042

Cited by 161 publications

(170 citation statements)

References 39 publications

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“…Jab1 belongs to the Mov-34 protein family whose members are involved in diverse functions involving macromolecular assembly (Asano et al, 1997). Jab1 has been originally identi®ed by its ability to bind to the c-Jun transactivation domain (Claret et al, 1996).…”

Section: Various Nuclear Co-factors Interact With Bcl-3mentioning

confidence: 99%

The Bcl-3 oncoprotein acts as a bridging factor between NF-κB/Rel and nuclear co-regulators

et al. 1999

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The proto-oncoprotein Bcl-3 is a member of the IkB family and is present predominantly in the nucleus. To gain insight into speci®c nuclear functions of Bcl-3 we have isolated proteins that interact with its ankyrin repeat domain. Using the yeast two-hybrid-system we identi®ed four novel binding partners of Bcl-3 in addition to NF-kB p50 and p52, previously known to associate with Bcl-3. The novel Bcl-3 interactors Jab1, Pirin, Tip60 and Bard1 are nuclear proteins which also bind to other transcription factors including c-Jun, nuclear factor I (NFI), HIV-1 Tat or the tumor suppressor and Polll holoenzyme component Brca1, respectively. Bcl-3, p50, and either Bard1, Tip60 or Pirin are sequestered into quarternary complexes on NF-kB DNA binding sites, whereas Jab1 enhances p50-Bcl-3-DNA complex formation. Furthermore, the histone acetylase Tip60 enhances Bcl-3-p50 activated transcription through an NF-kB binding site, indicating that quarternary complexes containing Bcl-3 interactors modulate NF-kB driven gene expression. These data implicate Bcl-3 as an adaptor between NF-kB p50/p52 and other transcription regulators and suggest that its gene activation function may at least in part be due to recruitment of the Tip60 histone actetylase.

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Section: Various Nuclear Co-factors Interact With Bcl-3mentioning

confidence: 99%

The Bcl-3 oncoprotein acts as a bridging factor between NF-κB/Rel and nuclear co-regulators

et al. 1999

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“…To narrow the TRC8 interaction domain in JAB1, we generated labeled proteins for amino acids 1 ± 187 containing the Nterminus and MPN protein interaction domain, the MPN domain alone (aa 55 ± 187), and the C-ter (aa 191 ± 327). Both proteins containing the MPN domain (Asano et al, 1997;Hofmann and Bucher, 1998;Ferrell et al, 2000) bound DTrc8 while the C-ter did not (Figure 4d, lower panels).…”

Section: Introductionmentioning

confidence: 99%

The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway

et al. 2002

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VHL is part of an SCF related E3-ubiquitin ligase complex with`gatekeeper' function in renal carcinoma. However, no mutations have been identi®ed in VHL interacting proteins in wild type VHL tumors. We previously reported that the TRC8 gene was interrupted by a t(3;8) translocation in a family with hereditary renal and non-medullary thyroid cancer. TRC8 encodes a multi-membrane spanning protein containing a RING-H2 ®nger with in vitro ubiquitin ligase activity. We isolated the Drosophila homologue, DTrc8, and studied its function by genetic manipulations and a yeast 2-hybrid screen. Human and Drosophila TRC8 proteins localize to the endoplasmic reticulum. Loss of either DTrc8 or DVhl resulted in an identical ventral midline defect. Direct interaction between DTrc8 and DVhl was con®rmed by GST-pulldown and co-immunoprecipitation experiments. CSN-5/JAB1 is a component of the COP9 signalosome, recently shown to regulate SCF function. We found that DTrc8 physically interacts with CSN-5 and that human JAB1 localization is dependent on VHL mutant status. Lastly, overexpression of DTrc8 inhibited growth consistent with its presumed role as a tumor suppressor gene. Thus, VHL, TRC8, and JAB1 appear to be linked both physically and functionally and all three may participate in the development of kidney cancer.

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“…The EIF3S3 gene encodes for a p40 subunit of eukaryotic translation initiation factor 3 (eIF3). The eIF3 complex plays a central role in the translation initiation pathway by binding to 40S ribosomal subunits in the absence of other translational components, and it helps to maintain the 40S and 60S subunits in a dissociated state (Asano et al, 1997). The function of the p40 subunit itself is, however, not known.…”

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confidence: 99%

Expression and copy number analysis of TRPS1, EIF3S3 and MYC genes in breast and prostate cancer

et al. 2004

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The long arm of chromosome 8 is one of the most common regions of amplification in cancers of several organs, especially carcinomas of the breast and prostate. TRPS1, MYC and EIF3S3 genes are located in one of the minimal regions of amplification, 8q23 -q24, and have been suggested to be the target genes of the amplification. Here, our goal was to study copy number and expression of the three genes in order to investigate the significance of the genes in breast and prostate cancer. By using fluorescence in situ hybridisation (FISH), we first found that TRPS1 and EIF3S3 were amplified together in about one-third of hormone-refractory prostate carcinomas. Next, we analysed the mRNA expression of the three genes by real-time quantitative RT -PCR and the gene copy number by FISH in six breast and five prostate cancer cell lines. Breast cancer cell line, SK-Br-3, which contained the highest copy number of all three genes, showed overexpression of only EIF3S3. Finally, the expression levels of TRPS1, EIF3S3 and MYC were measured in freshly frozen clinical samples of benign prostate hyperplasia (BPH), as well as untreated and hormone-refractory prostate carcinoma. The TRPS1 and MYC expression levels were similar in all prostate tumour groups, whereas EIF3S3 expression was higher (P ¼ 0.029) in prostate carcinomas compared to BPH. The data suggest that the expression of EIF3S3 is increased in prostate cancer, and that one of the mechanisms underlying the overexpression is the amplification of the gene.

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Structure of cDNAs Encoding Human Eukaryotic Initiation Factor 3 Subunits

Cited by 161 publications

References 39 publications

The Bcl-3 oncoprotein acts as a bridging factor between NF-κB/Rel and nuclear co-regulators

The Bcl-3 oncoprotein acts as a bridging factor between NF-κB/Rel and nuclear co-regulators

The TRC8 hereditary kidney cancer gene suppresses growth and functions with VHL in a common pathway

Expression and copy number analysis of TRPS1, EIF3S3 and MYC genes in breast and prostate cancer

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