Structure of CoFe2O4@CdTe nanocomposite with core/shell structure for high-performance Bi-modal imaging

Liu, Fujun; Elst, Luce Vander; Müller, Robert N.; Laurent, Sophie

doi:10.1016/j.colsurfa.2017.10.081

Cited by 4 publications

(1 citation statement)

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“…The observed weight lost below 200 °C was attributed to the evaporation of residual water in the sample and only the weight changes registered above 200 °C were considered as part of the organic components of it. The thermogram for pure sodium citrate showed three mass losses, one of which was attributed to the partial degradation of sodium citrate at around 310 °C and its residues at 450 °C (Supplementary Figure S4) (Marcilla et al, 2018;Liu et al, 2018). The thermogram for pure HS-PEG5K-NH 2 showed a derivative peak at 380 °C (Supplementary Figure S5) with a weight Normalized visible spectra (A) and dynamic light scattering (B) of as-prepared citrate-capped AuNP (Au 0 ), compared to the functionalized with thiolpolyethylene glycol (PEG)-amine, and conjugated with the peptides selected (P 1 to P 5 ) in PBS.…”

Section: Tga Characterizationmentioning

confidence: 99%

Synthesis and evaluation of gold nanoparticles conjugated with five antigenic peptides derived from the spike protein of SARS-CoV-2 for vaccine development

Farfán-Castro,

García-Soto,

Betancourt-Mendiola

et al. 2024

Front. Nanotechnol.

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Introduction: The development of innovative anti-COVID-19 vaccines is a need to ensure the population’s immunity worldwide, with broad protection against variants of concern and low cost as the main goals. Gold nanocarriers are potential entities that could aid in the development of innovative vaccines having thermal stability, high immunogenicity, and safety as the main attributes. Moreover, this approach could lead to adjuvant-free formulations, which will reduce the costs of vaccines.Methods: In this study, five peptides (P1, P2, P3, P4, and P5) corresponding to linear epitopes of the SARS-CoV-2 spike (S) protein were chemisorbed on gold nanoparticles (AuNP) of 20 nm, prefunctionalized with heterobifunctional polyethylene glycol, by using glutaraldehyde as crosslinker to generate nanovaccine prototypes.Results and discussion: The surface modification was confirmed by DLS with an increase of 31.7 ± 1.8 nm in the hydrodynamic diameter and an average ζ potential of −8.3 ± 2.2 mV in PBS (as excipient). The coupling concentration achieved was 23.7 ± 7.1 μg of peptide per mg AuNP. These AuNP-based conjugates showed no inherent toxicity in assays performed with HEK293T cells, in which a 100–1,000 μg/mL concentration range only led to a temporary decrease of up to 30% in cell viability after 48 h of treatment with restoration by 72 h. The immunogenicity of the conjugates produced was assessed in test mice subjected to three subcutaneous doses at 2-week intervals. Significant levels of IgM against each target peptide were observed at an early stage of the immunization scheme in all groups, reaching maximum levels after the second dose, whereas the IgG response increased after the third dose. The AuNP-P2, AuNP-P3, and AuNP-P5 conjugates induced the highest levels of IgG antibodies, lasting for at least 2 months after the last boost, with a predominance of the IgG1 subclass. Although the magnitude of the response induced by the gold conjugates was comparable to that with alum as adjuvant, these nanoconjugates induced a longer response. Our data support the use of AuNP as carriers in innovative vaccines against SARS-CoV-2.

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Section: Tga Characterizationmentioning

confidence: 99%