Structure of the O-specific polysaccharide from Burkholderia vietnamiensis strain LMG 6998

Gaur, Deepa; Wilkinson, Stephen G.

doi:10.1016/s0008-6215(96)90138-x

Cited by 9 publications

(7 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was the material used to obtain the polymeric fraction for structural studies. During mild acid hydrolysis (1% acetic acid, 100°C, 2.5 h) of the LPS, the suspension turned dark brown as noted in previous work [21]. Fractionation of the water‐soluble products on Sephadex G‐50 provided polymeric material giving a broad elution profile.…”

Section: Resultssupporting

confidence: 61%

“…LPS (yield, 565 mg) was recovered mainly (87%) from the aqueous phase after treatment of the defatted cell walls (2.12 g) with hot, aqueous phenol [20]. The polymeric fraction (yield, 38%) was released by mild acid hydrolysis and isolated by gel permeation chromatography on Sephadex G‐50 [21].…”

Section: Methodsmentioning

confidence: 99%

“…Monosaccharide composition was determined by paper chromatography and high‐pH anion‐exchange chromatography (Dionex) of the sugars, and by gas‐liquid chromatography (GLC) of the derived alditol acetates [21], after hydrolysis of the polymers with 2 M trifluoroacetic acid at 98°C for 16 h [22]. The absolute configuration of rhamnose was determined by GLC of the acetylated but‐2‐yl glycosides [23], and that of galactose by enzymatic analysis [13].…”

Section: Methodsmentioning

confidence: 99%

“…The absolute configuration of rhamnose was determined by GLC of the acetylated but‐2‐yl glycosides [23], and that of galactose by enzymatic analysis [13]. Methylation analysis and Smith degradation were carried out by following standard procedures [21]. NMR spectra for samples in D 2 O were recorded with either a JEOL JNM‐GX270 or a Varian DXR600S instrument with acetone (δ H 2.225, δ C 31.07) as internal reference.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Lipopolysaccharide from Burkholderia vietnamiensis strain LMG 6999 contains two polymers identical to those present in the reference strain for Burkholderia cepacia serogroup O4

Gaur

Wilkinson

2006

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

Lipopolysaccharide was isolated from strain LMG 6999 of Burkholderia vietnamiensis. Degradative and NMR spectroscopic studies established the presence of two polymeric fractions based on the following trisaccharide repeating units: I:-->3)-alpha-D-Galp-(1-->3)-beta-D-Galp-(1-->3)-beta-D-GalpNAc- (1-->; II:-->3)-alpha-D-GalpNAc-(1-->3)-beta-D-GalpNAc-(1-->4)- alpha-L-Rhap-(1-->. The same polymers have previously been found together in lipopolysaccharide from the reference strain for Burkholderia cepacia serogroup O4 and, individually, in those from B. cepacia serogroups C (I) and A (II).

show abstract

Section: Resultssupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Lipopolysaccharide from Burkholderia vietnamiensis strain LMG 6999 contains two polymers identical to those present in the reference strain for Burkholderia cepacia serogroup O4

Gaur

Wilkinson

2006

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, a 3‐deoxy‐2‐ manno ‐2‐octulosonic acid (Kdo)‐containing polysaccharide was characterized in B. pseudomallei [9] with the tetrasaccharide repeat structure →3)‐β‐ d ‐Gal p [2OAc]‐(1→4)‐α‐ d ‐Gal p ‐(1→3)‐β‐ d ‐Gal p ‐(1→5)‐β‐Kdo‐(2→. Nothing is known about the EPSs produced by nitrogen‐fixing Burkholderia species, although the structures of repeat units of the lipolysaccharide O‐chain from B. vietnamiensis strains [10,11] have been determined.…”

mentioning

confidence: 99%

Structure of an acidic exopolysaccharide produced by the diazotrophic endophytic bacterium Burkholderia brasiliensis

Mattos

Jones

Heise

et al. 2001

European Journal of Biochemistry

View full text Add to dashboard Cite

The structure of an exopolysaccharide (EPS) produced by Burkholderia brasiliensis, a diazotrophic endophytic organism originally isolated from rice roots, has been determined. The bacterium was grown in a synthetic medium, containing mannitol and glutamate, which favours the expression of two anionic EPSs, which were separated by anion-exchange chromatography. The structure of the repeat unit of EPS A, eluted at higher ionic strength, was determined by a combination of methylation analysis, partial hydrolysis, chemical degradations, and NMR spectroscopic studies, and shown to be the linear O-acetylated pentasaccharide: In this paper, we report the isolation and purification of two acidic EPSs (called EPS A and EPS B) synthesized by B. brasiliensis strain M 130, and report the structure of the repeat unit of EPS A. M A T E R I A L S A N D M E T H O D S

show abstract

Burkholderia

Palleroni¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Burk.hol.de' ri.a . M.L. fem. n. Burkholderia named after W.H. Burkholder, American bacteriologist who discovered the etiological agent of onion rot. Proteobacteria / Betaproteobacteria / Burkholderiales / Burkholderiaceae / Burkholderia Cells single or in pairs, straight or curved rods , but not helical. Dimensions, generally 0.5–1 × 1.5–4 µm. Motile by means of one or, more commonly, several polar flagella . One species ( Burkholderia mallei ) lacks flagella and is nonmotile. Do not produce sheaths or prosthecae. No resting stages are known. Gram negative. Most species accumulate poly‐β‐hydroxybutyrate (PHB) as carbon reserve material . Chemoorganotrophs. Have a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor . Some species can exhibit anaerobic respiration with nitrate. Strains of some of the species ( B. cepacia , B. vietnamiensis ) are able to fix N 2 . Catalase positive. A wide variety of organic compounds can be used as sources of carbon and energy for growth. Although hydroxylated fatty acids are present in the lipids of members of other genera of aerobic pseudomonads, species of Burkholderia are characterized by the presence of hydroxy fatty acids of 14, 16, and 18 carbon atoms (C 14:0 3OH and C 16:0 , and C 16:0 2OH , C 16:1 , and C 18:1 ) . The most characteristic of these acids is the C 16:0 3OH . Two different ornithine lipids are present in strains of some of the species. Over one‐half of the species are pathogenic for plants or animals (including humans) . The genus belongs to the ribosomal RNA similarity group II, which can be differentiated from other groups of aerobic pseudomonads by rRNA/DNA hybridization experiments or by rDNA sequencing. The mol% G + C of the DNA is : 59–69.6. Type species : Burkholderia cepacia (Palleroni and Holmes 1981) Yabuuchi, Kosako, Oyaizu, Yano, Hotta, Hashimoto, Ezaki and Arakawa 1993, 398 (Effective publication: Yabuuchi, Kosako, Oyaizu, Yano, Hotta, Hashimoto, Ezaki and Arakawa 1992, 1271) ( Pseudomonas cepacia Palleroni and Holmes 1981, 479.)

show abstract

Structure of the O-specific polysaccharide from Burkholderia vietnamiensis strain LMG 6998

Cited by 9 publications

References 21 publications

Lipopolysaccharide from Burkholderia vietnamiensis strain LMG 6999 contains two polymers identical to those present in the reference strain for Burkholderia cepacia serogroup O4

Lipopolysaccharide from Burkholderia vietnamiensis strain LMG 6999 contains two polymers identical to those present in the reference strain for Burkholderia cepacia serogroup O4

Structure of an acidic exopolysaccharide produced by the diazotrophic endophytic bacterium Burkholderia brasiliensis

Burkholderia

Contact Info

Product

Resources

About