Structured bottom section in inclined settlers for efficient continuous solid-liquid separation and washing of the solid fraction

Hammerschmidt, Nikolaus; Engelmaier, Hannah; Dattenböck, Christoph; Sencar, Jure; Jungbauer, Alois

doi:10.1016/j.seppur.2020.118142

Cited by 2 publications

(6 citation statements)

References 18 publications

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“…We have previously introduced a structured‐design bottom section for inclined plate settlers 23 . In summary, this novel concept significantly enhances solid–liquid separation efficiency by enabling the implementation of a wash step.…”

Section: Resultsmentioning

confidence: 99%

“…The inclined settler was comprised of four settling channels that were inclined by 60° relative to the horizontal. The bottom section was built according to the structured design for inclined settler bottom sections 23 . The total internal hold‐up volume of the settler and bottom section was 830 mL.…”

Section: Methodsmentioning

confidence: 99%

“…The bottom section was built according to the structured design for inclined settler bottom sections. 23 The total internal hold-up volume of the settler and bottom section was 830 mL. The wash buffer was PBS-C (14 g L −1 NaCl, 0.2 g L −1 KH 2 PO 4 , 1.15 g L −1 Na 2 HPO 4 , pH 7.0).…”

Section: Cell Removalmentioning

confidence: 99%

“…22 For continuous solidliquid separation a number of solutions have been proposed. These include tangential flow filtration, fluidized bed centrifugation, hydro-cyclones and gravity separation, 23 each of which have their advantages and disadvantages. 9,21,22,24 The availability of suitable solid-liquid separation solutions is crucial for the overall feasibility of continuous precipitation.…”

Section: Introductionmentioning

confidence: 99%

“…For continuous solid–liquid separation a number of solutions have been proposed. These include tangential flow filtration, fluidized bed centrifugation, hydro‐cyclones and gravity separation, 23 each of which have their advantages and disadvantages 9,21,22,24 …”

Section: Introductionmentioning

confidence: 99%

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Intensified capture of recombinant blood coagulation factors by continuous, integrated downstream processing

Engelmaier,

Dattenböck,

Petrushevska‐Seebach

et al. 2023

J of Chemical Tech & Biotech

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BACKGROUNDThe biopharmaceutical community has realized the tremendous potential of continuous processing for process intensification. However, to date reports of continuous processes for monoclonal antibodies (mAb) represent the overwhelming majority, while integrated continuous processes for non‐mAb products are rare.RESULTSWe have developed an integrated, continuous process for capture of recombinant blood coagulation factors: Factor VIII (FVIII) and VonWillebrand factor (VWF). Due to their complex and diverse nature, tailored solutions beyond chromatographic separations are required for continuously producing non‐mAb products. Our integrated process consists of three continuous unit operations that include cell removal, product capture by precipitation and precipitate collection with a yield of 33% and 56% for FVIII and VWF, respectively. These unit operations were developed individually and then integrated into a continuous process at laboratory scale. Using prototype equipment, we have demonstrated continuous, integrated capture of recombinant blood coagulation factors over a duration of 24 h.CONCLUSIONThe continuous solutions employed were developed for FVIII and VWF, but may very well be applied to other molecules. These solutions to continuous solid–liquid separation and integration are important parts in the puzzle that is continuous downstream processing of biopharmaceuticals. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cell Removalmentioning

confidence: 99%