Structures of a DNA Polymerase Caught while Incorporating Responsive Dual‐Functional Nucleotide Probes
Pulak Ghosh,
Karin Betz,
Cédric Gutfreund
et al.
Abstract:Functionalizing nucleic acids using DNA polymerases is essential in biophysical and biotechnology applications. This study focuses on understanding how DNA polymerases recognize and incorporate nucleotides with diverse chemical modifications, aiming to develop advanced nucleotide probes. We present the crystal structures of ternary complexes of Thermus aquaticus DNA polymerase (KlenTaq) with C5‐heterocycle‐modified environment‐sensitive 2′‐deoxyuridine‐5′‐triphosphate (dUTP) probes. These nucleotides include S… Show more
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