StuA is required for cell pattern formation in Aspergillus.

Miller, Karen Y.; Wu, Jiang; Miller, Bruce L.

doi:10.1101/gad.6.9.1770

Cited by 190 publications

(225 citation statements)

References 52 publications

Supporting

Mentioning

210

Contrasting

Unclassified

Order By: Relevance

“…nidulans For the targeting of GFP to nuclei, the A. nidulans StuA protein was used. The stuA gene encodes a putative transcription factor that is involved in the regulation of conidiophore development (Miller et al, 1992). At the carboxyterminus of the protein, a putative bipartite nuclear localization domain was detected.…”

Section: Resultsmentioning

confidence: 99%

Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans

Suelmann

Sievers

Fischer

1997

Molecular Microbiology

175

140

View full text Add to dashboard Cite

SummaryNuclear migration and nuclear positioning are fundamental processes in all eukaryotic cells. They are easily monitored during hyphal growth of filamentous fungi. We expressed the green fluorescent protein (GFP) as a fusion protein with the putative nuclear localization domain of the transcriptional activator stuA in nuclei of Aspergillus nidulans and visualized these organelles in living cells. Nuclear staining was observed in interphase nuclei but not during mitosis. Nuclear division, nuclear migration, septum formation and branching were analysed with time-lapse video microscopy during hyphal extension. Hyphae elongated at 0.1-1.2 m min ¹1 and nuclei moved with similar speeds towards the hyphal tip until they had reached a defined position. An individual regulation of nuclear mobility in a given hyphal compartment was observed. Some representative movies are available on the Internet (http:/ /www.blacksci.co.uk/products/journals/molextra.htm). Nuclear positioning was also studied at the molecular level. The ApsA protein, which regulates nuclear migration, was localized at the cytoplasmic membrane in germlings and hyphae by immunofluorescence and GFP tagging. A model of nuclear migration, nuclear positioning and the role of ApsA is presented.

show abstract

Section: Resultsmentioning

confidence: 99%

Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans

Suelmann

Sievers

Fischer

1997

Molecular Microbiology

175

140

View full text Add to dashboard Cite

show abstract

“…Several key regulatory genes have been identified and cloned and shown to control progression through the various stages of conidiation (28)(29)(30)(31). Properties of the corresponding regulatory proteins and the sites at which some of them act have been determined (32,33).…”

Section: Discussionmentioning

confidence: 99%

Mutants of Neurospora crassa that alter gene expression and conidia development.

Madi

Ebbole²,

White³

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Several genes have been identied that are highly expressed during conidiation. Inactivation ofthese genes has no observable phenotypic effect. Transcripts of two such genes, con-6 and con-10, are norally absent from vegetative mycella. To identify regulatory genes that affect con-6 and/or con-10 expression, strains were prepared in which the regulatory regions for these genes were fused to a gene conferrin hygromycin resistance. Mutants were then selected that were resistant to the drug during mycelial growth. Mutations in several of the isolates had trans effects; they activated transcription of the corresponding intact gene and, in most oates, one or more ofthe other con genes. Most Analyses of the protein and mRNA species present in mycelia, aerial hyphae, and conidia have revealed significant differences in gene expression (3)(4)(5). A differential screening procedure was used to clone genes that are preferentially expressed during conidiation (4). Four of these genes, con-6, con-8, con-10, and con-13, have been sequenced, and their expression has been examined (6-10). A fifth conidiationspecific gene, eas, also has been characterized (11, 12); eas encodes the rodlet protein that coats the surface ofthe mature conidium.con-6 and con-10 encode 93-and 86-residue polypeptides, respectively; the functions of these polypeptides are unknown. con-6 and con-10 transcripts and proteins are normally undetectable in vegetative mycelium but appear at high levels during conidiation and in mature conidia; these transcripts and proteins disappear a few hours after conidia germinate (6,13). The expression pattern of these genes closely parallels that of eas (11, 12). The con-6 and con-10 proteins are present in a second type of asexual spore, the microconidium (10,13), and the con-10 protein is present in sexual spores (10, 13). Expression of con-6 and con-10 can also be induced in vegetative mycelial cultures by light exposure or by imposing conditions that induce circadian rhythm (10,(13)(14)(15). We describe here the selection and partial characterization of mutants that express con-6 and con-10 aberrantly during vegetative mycelial growth. Some of these mutants were found to be blocked in conidiation, whereas others exhibited different developmental abnormalities. MATERIALS AND METHODSStrains and Pids. Strain CH10 was constructed by homologous integration, using pCH10, a plasmid containing a con-10 translational fusion to the hygromycin phosphotransferase gene (hph) (16). Integration was at the his-3 locus of Fungal Genetics Stock Center (FGSC) strain 462: his-3 A. The integrated plasmid consisted of pBluescript KS+ (Promega) vector containing con-10 DNA from positions 1139 to 1935 (8); this segment contains the first 40 codons of con-10. The con-10 DNA is fused in-frame to the second codon of hph followed by the Aspergillus nidulans trpC transcription termination region from plasmid pDH25 (16). The plasmid also contains a 5' truncated copy of the his-3 gene that allows reconstitution of an intact copy of the ...

show abstract

“…Several hyphal inducing media were used: Lee's medium (36) with 1% mannitol as the carbon source, YPD ϩ 10% serum, SS (synthetic succinate) medium (0.0425% YNB without amino acids and ammonium sulfate (Difco), 0.125% ammonium sulfate, 2% succinic acid, pH 6.5), and SSA (SS with amino acids) medium. A modified lithium acetate method was used for C. albicans transformation (34).…”

Section: Methodsmentioning

confidence: 99%

“…The fact that AbaA expression is under the direct control of StuA in A. nidulans (36), and that AbaA/Tec1-binding sites are found in the upstream regions of all known hypha-specific and serum-induced genes (Table IV), led us to investigate the possibility that Efg1 may regulate TEC1 transcription. We examined TEC1 expression in efg1/efg1 mutants by Northern analysis.…”

Section: Cph1 Cph2 and Efg1 Regulate The Same Set Of Differentiallymentioning

confidence: 99%

DNA Array Studies Demonstrate Convergent Regulation of Virulence Factors by Cph1, Cph2, and Efg1 in Candida albicans

Lane¹,

Birse²,

Zhou³

et al. 2001

Journal of Biological Chemistry

208

169

View full text Add to dashboard Cite

Candida albicans, normally a human commensal, can cause fatal systemic infections under certain circumstances. Its unique ability to switch from yeast to hyphal growth in response to various environmental signals is inherent to its pathogenicity. Filamentation is regulated by multiple pathways including a Cph1-mediated mitogen-activated protein kinase pathway, an Efg1-mediated cAMP/PKA pathway, and a Cph2 pathway. To gain a general picture of how these various signaling pathways regulate differential gene expression during filamentation, we have constructed a partial C. albicans DNA array of 7,000 genes and used it to study the gene expression profiles using various mutants and growth conditions. By combining this novel technology with a new liquid medium in which cph1/cph1 is defective in filamentation, previously identified differentially expressed genes (ECE1, HWP1, HYR1, RBT1, SAPs5-6, and RBT4) are found to be regulated by all three pathways. In addition, two novel genes, DDR48 and YPL184, have been found to be differentially regulated during hyphal development and by all three pathways. This suggests that distinct filamentation signaling pathways converge to regulate a common set of differentially expressed genes. As one of the mechanisms for the observed convergence, we find that the transcription of a key regulator, TEC1, is regulated by Efg1 and Cph2. Importantly, most of the genes regulated by multiple filamentation pathways encode known virulence factors. Perhaps, C. albicans utilizes converging pathways to regulate its vital virulence factors to ensure its survival and pathogenicity in various host environments.

show abstract

StuA is required for cell pattern formation in Aspergillus.

Cited by 190 publications

References 52 publications

Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans

Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans

Mutants of Neurospora crassa that alter gene expression and conidia development.

DNA Array Studies Demonstrate Convergent Regulation of Virulence Factors by Cph1, Cph2, and Efg1 in Candida albicans

Contact Info

Product

Resources

About