Studies of a novel membrane for affinity separations

Bamford, C. H.; Al-Lameé, Kadem; Purbrick, M. D.; Wear, Trevor J.

doi:10.1016/0021-9673(92)85253-p

Cited by 56 publications

(30 citation statements)

References 4 publications

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“…Currently, SUMs used for preparation of dipsticks are either glued together with the two microfiltration membranes facing each other or attached to polymer films (Mader, unpublished observation). Contrary to conventionally used microfiltration membranes, which can adsorb up to milligrams of proteins per cm 2 membrane area (1,5), the surface of SUMs revealed extremely low nonspecific adsorption and nearly no background without blocking steps in immunological reactions. The main reason for this advantage is seen in the well-defined physicochemical and morphological properties of S-layer lattices composed of identical protein subunits.…”

Section: Discussionmentioning

confidence: 99%

2-D Protein Crystals as an Immobilization Matrix for Producing Reaction Zones in Dipstick-Style Immunoassays

Breitwieser¹,

Küpcü²,

Howorka³

et al. 1996

BioTechniques

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Section: Discussionmentioning

confidence: 99%

2-D Protein Crystals as an Immobilization Matrix for Producing Reaction Zones in Dipstick-Style Immunoassays

Breitwieser¹,

Küpcü²,

Howorka³

et al. 1996

BioTechniques

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“…Using a simple model for monolayer packing of spherical particles, such as that described by Bamford et al [37], the maximum theoretical surface density for apoB can be calculated as 1.7 mg/m 2 surface area. This calculation makes use of the facts that LDL are essentially spherical particles approximately 250 Å in diameter [30,38], and that each LDL particle contains a single molecule of apoB [30].…”

Section: Discussionmentioning

confidence: 99%

In vitro Characterization of a Membrane-Based Low-Density Lipoprotein Affinity Adsorption Device

Soltys

Etzel²

1998

Blood Purif

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The objective of this study was to explore the use of microporous membranes as an alternative substrate to porous beads in affinity adsorption of low-density lipoprotein (LDL) for therapeutic purposes. Flat sheet immunoaffinity membranes containing a polyclonal antibody preparation were utilized as the affinity substrate. The antibody was covalently immobilized to the surface through a poly(ethylene glycol) (PEG) spacer. Equilibrium adsorption of LDL from plasma was measured. Adsorption from plasma and elution of bound LDL using citrate buffer were studied as a function of flow rate.Specific capacity was as great as 2 mg apolipoprotein B per milliliter membrane volume. The superior transport properties of the membrane allowed rapid adsorption and regeneration, which translated to a large number of adsorptive cycles that can be performed within a given treatment time. On the basis of in vitro performance characteristics, it is estimated that an immunoaffinity membrane device can provide a reduction in patient plasma LDL concentration comparable to that provided by packed columns, but with almost an 80% reduction in the device volume.

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“…The fibers generated using this method exhibit excellent attributes, such as very large surface to volume ratios and a high porosities with a small pore size (23). Because of these attractive properties, electrospun nanofibers have been used in biomedical sciences, filtration, optical sensors, and affinity membranes (24)(25)(26) Previous studies have demonstrated that preformed MIP particles can be incorporated into membranes (22) and due to a high productivity of membranes, a high surface-to-volume ratio, and high porosities with a small pore size which could be increase separation efficiency and the inherent selectivity of MIP. Thus, the objective of this study was to develop a novel material for affinity membrane separation using electrospun fiber containing PPL-selective MIP.…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of Propranolol Selective Molecular Imprinted Polymer Composite Electrospun Nanofiber Membrane

et al. 2013

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Abstract. Propranolol (PPL) imprinted microspheres (MIP) were successfully prepared via oil/water polymerization using a methyl methacrylate (MMA) monomer, PLL template, and divinylbenzene (DVB) cross-linker and favorably incorporated in a Eudragit-RS100 nanofiber membrane. A non-PPL imprinted polymer (NIP), without a template, was used as a control. The morphology and particle size of the beads were investigated using scanning electron microscopy. The results revealed that both MIP and NIP had a spherical shape with a micron size of approximately 50-100 μm depending on the amounts of DVB and PPL used. NIP2 (MMA/DVB, 75:2.5) and MIP8 (PPL/MMA/DVB, 0.8:75:2.5) were selected for reloading of PPL, and the result indicated that increasing the ratio of PPL to polymer beads resulted in increase PPL reloading (>80%). A total of 10-50% NIP2 or MIP8 was incorporated into a 40%(w/v) Eudragit-RS100 fiber membrane using an electrospinning technique. PPL could be bound to the 50% MIP8 composite fiber membrane with a higher extent and at a higher rate than the control (NIP2). Furthermore, the MIP8 composite fiber membrane showed higher selectivity to PPL than the other β-blockers (atenolol, metoprolol, and timolol). Thus, the MIP8 composite fiber membrane can be further developed for various applications in pharmaceutical and other affinity separation fields.

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Studies of a novel membrane for affinity separations

Cited by 56 publications

References 4 publications

2-D Protein Crystals as an Immobilization Matrix for Producing Reaction Zones in Dipstick-Style Immunoassays

2-D Protein Crystals as an Immobilization Matrix for Producing Reaction Zones in Dipstick-Style Immunoassays

In vitro Characterization of a Membrane-Based Low-Density Lipoprotein Affinity Adsorption Device

Development and Characterization of Propranolol Selective Molecular Imprinted Polymer Composite Electrospun Nanofiber Membrane

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