Studies of Nucleic Acid Synthesis in Ascites Tumor Cells

LePage, G. A.; Greenlees, Janet; Fernandes, J.F.

doi:10.1111/j.1749-6632.1956.tb50907.x

Cited by 17 publications

(3 citation statements)

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“…wet wt.) Controls azaserine Uracil 87 90 120 128 Cytosine 1-2 1-2 15 20 about 75 % was produced by the addition of about 0-1 ,um-mole of azaserine in vitro ; LePage, Greenlees & Fernandes, 1956). Glutamine was able to prevent this inhibition only partly when added before the azaserine and at about 1000 times its concentration.…”

Section: Discussionmentioning

confidence: 99%

The Uptake of Guanine, Uracil, Lysine and Glycine by Ascites Cells Pretreated With Azaserine and 6-Thioguanine

Adams¹

1963

Biochemical Journal

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Section: Discussionmentioning

confidence: 99%

The Uptake of Guanine, Uracil, Lysine and Glycine by Ascites Cells Pretreated With Azaserine and 6-Thioguanine

Adams¹

1963

Biochemical Journal

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“…Inhibition of nucleic acid synthesis in yeast by phosphate deprivation does not prevent a doubling of the protein content (267), and glycine in corporation into the protein of ascites cells is not inhibited when incorpora tion of glycine into purines is stopped by azaserine (199). Methionine de privation in E. coli K12W6 permits an 80 per cent increase in RNA but little or no protein or adaptive enzyme synthesis (27) ; unlike strain 1ST-the cells remain viable during this period.…”

Section: Biological Involvementsmentioning

confidence: 96%

The Nucleic Acids

Cohn¹,

Volkin²

1957

Annu. Rev. Biochem.

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Previous reviews of this topic have summarized progress in the biosyn thesis (43), preparation (5), an d organic chemistry of nucleic acids and nucleotides (37). This essay is concerned with recent work that deals with nucleic acid structure, relation to physiological and metabolic events, and biological interventions. The large increase in the number of published papers is met by an attempt to summarize rather than to expound'in detail. STRUCTURE AND CHEMISTRYThe ability to prepare DNA from various sources in a more purified and less degraded form and ever-increasing apparent molecular weight (100), coupled with an appreciation of the roles of hydrogen bonding and internal charge distribution, which underlie the structure proposed by Watson & Crick (305), have inspired re-examinations of the physicochemical charac teristics of DNA with improved methodology.Titration.-More accurate determination of the pC' values of the effec tive groups in herring sperm DNA by Cox et al. (71) indicates essentially no phosphoryl titration in the neutral range, which eliminates consideration of secondary phosphoryl end groups and thus the need to invoke triply esteri fied phosphate. The hysteresis (difference between forward and backward titrations) in the titration of both herring sperm (71) and thymus (48, 166) DNA is ascribed to the normal (A to T, C to G) hydrogen bonding, which is eliminated at the extremes of pH, in accordance with theory. The increase in ultraviolet absorption (hyperchromic effect) and bathochromic shift in the spectrum after DNA has been carried to pH 2 or 13 is also in accord (191). It has been indicated by Cavalieri & Rosenberg (46) that the ac ceptance of a proton by guanine, the apparent pK of which varies consider ably with ionic strength (46, 166), may cause rearrangements in the purine that result in breaking both guanine-cytosine hydrogen bonds. The same reaction is held accountable by Cavalieri et al. for a spectrophotometrically 1 The survey of the literature pertaining to this review was completed in Novem ber, 1956.2 The following standard abbreviations are employed: RNA and DNA for the ribo-and deoxyribonucleic acids; A, G, C, MC, D, and T for adenine, guanine, cytosine, methylcytosine, uracil, and thymine; AMP, ADP, etc., for the corresponding nucleoside 5'-phosphates and -pyrophosphates; and TMV for tobacco mosaic virus.• Our thanks are due to Dr. K. Sebesta, of the Czechoslovak Academy of Sciences, for critical sum�ries of pertinent papers appearing in some forty journals of Central European countries. 4 Manuscript prepared under Contract No. W-7405-eng-26 for the Atomic Energy Commission. 491 Annu. Rev. Biochem. 1957.26:491-522. Downloaded from www.annualreviews.org Access provided by University of Western Ontario on 02/07/15. For personal use only. Quick links to online content Further ANNUAL REVIEWS

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“…LePage and Sartorelli (1957) showed that at therapeutic levels, azaserine inhibited the amidation of formylglycinamide ribotide to the amidine by irreversibly combining with the enzyme. DON also combined with the first enzyme of purine synthesis (Hartman, 1963).…”

Section: Metabolism and Metabolic Effectsmentioning

confidence: 98%