Studies of the interaction between paraquat and bovine hemoglobin

Wang, Yanqing; Zhang, Hongmei; Zhang, Gencheng; Liu, Shuang-Xia; Zhou, Qinqin; Fei, Zhenghao; Liu, Zongtang

doi:10.1016/j.ijbiomac.2007.02.011

Cited by 186 publications

(63 citation statements)

References 49 publications

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“…Synchronous fluorescence spectra were developed in 1971 and were obtained by simultaneously scanning excitation and emission monochromators while maintaining a constant wavelength interval between them [44]. When the D-value (Δλ) between excitation and emission wavelength were set at 15 or 60 nm, the synchronous fluorescence Fig.…”

Section: Conformational Investigationsmentioning

confidence: 99%

Molecular Interactions of Flavonoids to Hyaluronidase: Insights from Spectroscopic and Molecular Modeling Studies

Zeng

Yang

et al. 2015

J Fluoresc

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In the work described on this paper, the interactions between eight flavonoids and hyaluronidase (HAase), an important enzyme involved in a promoting inflammation pathway, were investigated by spectroscopic and molecular modeling methods. The results revealed that all flavonoids could interact with HAase to form flavonoid-HAase complexes. The binding parameters obtained from the data at different temperatures indicated that flavonoids could spontaneously bind with HAase mainly through electrostatic forces and hydrophobic interactions with one binding site. According to synchronous and three-dimensional fluorescence spectra and the molecular docking results, all flavonoids bound directly into the enzyme cavity site and the binding of flavonoid into the enzyme cavity influenced the microenvironment of the HAase activity site which led to the reduced enzyme activity. The present study provides direct evidence at a molecular level to understand the mechanism of inhibitory effect of flavonoid against HAase and explain the anti-inflammatory mechanism of the Traditional Chinese Medicines as anti-inflammatory drugs.

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Section: Conformational Investigationsmentioning

confidence: 99%

Molecular Interactions of Flavonoids to Hyaluronidase: Insights from Spectroscopic and Molecular Modeling Studies

Zeng

Yang

et al. 2015

J Fluoresc

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“…The shift in the position of maximum emission wavelength reflects the change of the polarity around chromophore molecule [27]. When the wavelength interval (Dk) between the excitation and emission wavelength is stabilized at 15 and 60 nm, the synchronous fluorescence offers the characteristics of Tyr and Trp residues [28].…”

Section: Conformational Investigationsmentioning

confidence: 99%

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Cheng

2012

Mol Biol Rep

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This study was designed to examine the interactions of ergosterol with bovine serum albumin (BSA) and human serum albumin (HSA) under physiological conditions with the drug concentrations in the range of 2.99-105.88 μM and the concentration of proteins was fixed at 5.0 μM. The analysis of emission spectra quenching at different temperatures revealed that the quenching mechanism of HSA/BSA by ergosterol was the static quenching. The number of binding sites n and the binding constants K were obtained at various temperatures. The distance r between ergosterol and HSA/BSA was evaluated according to Föster non-radioactive energy transfer theory. The results of synchronous fluorescence, 3D fluorescence, FT-IR, CD and UV-Vis absorption spectra showed that the conformations of HSA/BSA altered in the presence of ergosterol. The thermodynamic parameters, free energy change (ΔG), enthalpy change (ΔH) and entropy change (ΔS) for BSA-ergosterol and HSA-ergosterol systems were calculated by the van't Hoff equation and discussed. Besides, with the aid of three site markers (for example, phenylbutazone, ibuprofen and digitoxin), we have reported that ergosterol primarily binds to the tryptophan residues of BSA/HSA within site I (subdomain II A).

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“…The results were expressed in terms of mean residue ellipticity (MRE; in deg cm 2 dmol −1 ) according to the following equation [32][33][34][35],…”

Section: Circular Dichroism Spectramentioning

confidence: 99%

Spectroscope and molecular model identify the behavior of doxorubicin-SPION binding to bovine hemoglobin

Liu

2015

International Journal of Biological Macromolecules

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Studies of the interaction between paraquat and bovine hemoglobin

Cited by 186 publications

References 49 publications

Molecular Interactions of Flavonoids to Hyaluronidase: Insights from Spectroscopic and Molecular Modeling Studies

Molecular Interactions of Flavonoids to Hyaluronidase: Insights from Spectroscopic and Molecular Modeling Studies

Interaction of ergosterol with bovine serum albumin and human serum albumin by spectroscopic analysis

Spectroscope and molecular model identify the behavior of doxorubicin-SPION binding to bovine hemoglobin

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