Studies on Attenuation of Post-ischemic Brain Injury by Kampo Medicines-Inhibitory Effects of Free Radical Production. I

Fushitani, Shuji; Tsuchiya, Koichiro; Minakuchi, Kazuo; Takasugi, Masumitsu; Murakami, Kōtarō

doi:10.1248/yakushi1947.114.6_388

Cited by 10 publications

(3 citation statements)

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“…We have previously reported that KBG preserves the endothelium-dependent relaxation of the thoracic aorta in cholesterol-fed rabbits by limiting superoxide generation, 25) and it is known that OGT also scavenges oxygen free radicals such as superoxide radicals and hydroxyl radicals in vitro. 26,27) Moreover, it is reported that the effect of OGT for inhibiting enzymic lipid peroxidation in rat liver microsomes consists of not only antioxidant action but also of reduction in the supply of electrons derived from nicotinamide adenine dinucleotide phosphate (NADPH) to the ADP · Fe 3ϩ complex in a competitive manner, as well as stimulation of NADPH oxidation. 28) So this mechanism might represent a difference between OGT and KBG.…”

Section: Fig 1 Effects Of Kampo Medicines On Progression Of Atherommentioning

confidence: 99%

Oren-gedoku-to and Keishi-bukuryo-gan-ryo Inhibit the Progression of Atherosclerosis in Diet-Induced Hypercholesterolemic Rabbits

Sekiya

Kainuma

Hikiami

et al. 2005

Biological & Pharmaceutical Bulletin

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Many investigations have indicated that elevated serum cholesterol levels may modify the biochemical properties of blood components and arterial intima, thus enhancing the progression of atherosclerosis.1-3) Atherosclerosis brings about cerebrovascular disease and coronary artery disease. The cholesterol-fed rabbit is an animal model for hypercholesterolemia and atheromatous formation. Formerly, we clarified that Keishi-bukuryo-gan-ryo (KBG, Gui-zhi-fu-ling-wan in Chinese) prevents the development of atheromatous plaque formation in the aorta of cholesterol-fed rabbits by its antioxidative effect. 4) Vitamin E is a lipid-soluble antioxidant that inhibits lipid peroxidation and attenuates atherosclerosis. However, other studies showed that vitamin E might be a pro-oxidant. 5-7)Oren-gedoku-to (OGT, Huanlian-jie-du-tang in Chinese) is a Kampo formulation consisting of four medicinal plants (Table 1). It has been used for the treatment of cerebrovascular disease, hypertension, gastritis and liver disease in Japan. Several studies have shown that OGT scavenges superoxide anions and hydroxyl radicals in vitro. 8,9) Furthermore, it has been revealed that ingestion of OGT strengthens the protection of RBC membrane in oxidative stress, and improves protein and lipid metabolisms in human. 10,11) In this study, we investigated whether OGT is able to inhibit the progression of atheromatous formation in cholesterol-fed rabbits. Moreover, we compared the effect of OGT with those of KBG and vitamin E. MATERIALS AND METHODS Reagents. Medicines OGT (TJ-15) and KBG (TJ-25)were kindly provided by Tsumura & Co. Powdered extract of OGT was obtained from 4 kinds of medicinal plants mixed at the ratios shown in Table 1, and that of KBG was from 5 medicinal plants (Table 2). These nine medicinal plants are registered in the Japanese Pharmacopoeia.Animals Male Japanese white rabbits weighing 2 kg were purchased from Sankyo Laboratories (Toyama, Japan). Twenty-four rabbits were randomly divided into four groups. Medicine, Oriental Medical Center, Iizuka Hospital; Iizuka, Japan: c Department of Kampo Diagnostics, Institute of Wakan-yaku, Toyama Medical and Pharmaceutical University; Sugitani 2630, Toyama 930-0194, Japan: and d 21st Century COE Program, Toyama Medical and Pharmaceutical University; Sugitani 2630, Toyama 930-0194, Japan. Received September 9, 2004 accepted November 12, 2004; published online November 18, 2004 In this study, we examined whether the Kampo formulas Oren-gedoku-to (OGT, Huanglian-jie-du-tang in Chinese) and Keishi-bukuryo-gan-ryo (KBG, Gui-zhi-fu-ling-wan in Chinese) could prevent the progression of atherosclerosis in cholesterol-fed rabbit, an animal model for hypercholesterolemia in vivo. Twenty-four male Japanese white rabbits (2 kg body weight) were divided into four groups. The control group was fed standard rabbit chow containing 1% cholesterol, the OGT group was fed standard rabbit chow containing 1% cholesterol and 1% OGT, the KBG group was fed standard rabbit chow containing 1% cholesterol an...

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Section: Fig 1 Effects Of Kampo Medicines On Progression Of Atherommentioning

confidence: 99%

Oren-gedoku-to and Keishi-bukuryo-gan-ryo Inhibit the Progression of Atherosclerosis in Diet-Induced Hypercholesterolemic Rabbits

Sekiya

Kainuma

Hikiami

et al. 2005

Biological & Pharmaceutical Bulletin

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“…[8][9][10] It has been indicated that oxygen free radicals such as superoxide radical, hydroxyl radical, and singlet oxygen participate in enzymatic lipid peroxidation in rat liver microsomes. [27][28][29][30][31][32][33][34] We have observed that when the effects of superoxide dismutase, catalase, hydroxyl radical scavengers such as mannitol, sodium formate, and dimethylthiourea, and singlet oxygen quenchers such as histidine, diazabicyclooctane, and diphenylfuran, on enzymatic lipid peroxidation in rat liver microsomes used in the present study are examined, singlet oxygen quenchers inhibit peroxidation by more than 90%, while superoxide dismutase, catalase, and hydroxyl radical scavengers inhibit it by less than 20% (unpublished data).…”

Section: Fig 5 Effect Of Late Addition Of Tj-15 On Enzymatic Lipid mentioning

confidence: 99%

“…[8][9][10] 12) It has also been reported that TJ-15 inhibits lipid peroxidation induced by reduced b-nicotinamide adenine dinucleotide phosphate (NADPH), cumene hydroperoxide, or iron/ascorbate in rat liver microsomes. 13) We have shown that TJ-15 inhibits lipid peroxidation induced by ADP/Fe 3ϩ -NADPH, carbon tetrachloride, cumene hydroperoxide, or the water-soluble radical initiator 2,2Ј-azobis(2-amidinopropane) in rat liver microsomes.…”

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confidence: 99%

Inhibitory Action of Oren-gedoku-to Extract on Enzymatic Lipid Peroxidation in Rat Liver Microsomes.

Hayashi

Ohta

Inagaki

et al. 2001

Biological & Pharmaceutical Bulletin

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Ameliorating effects of traditional Chinese medicine preparation, Chinese materia medica and active compounds on ischemia/reperfusion-induced cerebral microcirculatory disturbances and neuron damage

Sun

Fan

Han

2015

Acta Pharmaceutica Sinica B

203

121

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Ischemic stroke and ischemia/reperfusion (I/R) injury induced by thrombolytic therapy are conditions with high mortality and serious long-term physical and cognitive disabilities. They have a major impact on global public health. These disorders are associated with multiple insults to the cerebral microcirculation, including reactive oxygen species (ROS) overproduction, leukocyte adhesion and infiltration, brain blood barrier (BBB) disruption, and capillary hypoperfusion, ultimately resulting in tissue edema, hemorrhage, brain injury and delayed neuron damage. Traditional Chinese medicine (TCM) has been used in China, Korea, Japan and other Asian countries for treatment of a wide range of diseases. In China, the usage of compound TCM preparation to treat cerebrovascular diseases dates back to the Han Dynasty. Even thousands of years earlier, the medical formulary recorded many classical prescriptions for treating cerebral I/R-related diseases. This review summarizes current information and underlying mechanisms regarding the ameliorating effects of compound TCM preparation, Chinese materia medica, and active components on I/R-induced cerebral microcirculatory disturbances, brain injury and neuron damage.

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Studies on Attenuation of Post-ischemic Brain Injury by Kampo Medicines-Inhibitory Effects of Free Radical Production. I

Cited by 10 publications

References 0 publications

Oren-gedoku-to and Keishi-bukuryo-gan-ryo Inhibit the Progression of Atherosclerosis in Diet-Induced Hypercholesterolemic Rabbits

Oren-gedoku-to and Keishi-bukuryo-gan-ryo Inhibit the Progression of Atherosclerosis in Diet-Induced Hypercholesterolemic Rabbits

Inhibitory Action of Oren-gedoku-to Extract on Enzymatic Lipid Peroxidation in Rat Liver Microsomes.

Ameliorating effects of traditional Chinese medicine preparation, Chinese materia medica and active compounds on ischemia/reperfusion-induced cerebral microcirculatory disturbances and neuron damage

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