Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites

Li, Xi; Yang, Li‐Tao; Wang, Yunfei; Du, Zhongyu; Mao, Xuyan; Sun, Dezhi; Li, Jun; Yu, Zhou; Xu, Xiangyu

doi:10.1049/iet-nbt.2019.0377

Cited by 6 publications

(3 citation statements)

References 53 publications

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“…ssDNA has a much higher binding efficiency on the GO surface than double-stranded (ds) DNA. There are numerous approaches where GO operates as a support nanomaterial for detecting DNA, including electrochemical [ 13 , 14 ], field-effect transistors [ 15 ], and fluorescent biosensor applications [ 16 , 17 ]. Among these, fluorescent base biosensors have been of particular interest due to their enhanced capabilities, such as low cost, simplicity, high sensitivity, superior selectivity, fast response, and multiple analyses [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Toader,

Nitu,

Ionita

2023

Molecules

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A nitrocellulose–graphene oxide hybrid that consists of a commercially nitrocellulose (NC) membrane non-covalently modified with graphene oxide (GO) microparticles was successfully prepared for oligonucleotide extraction. The modification of NC membrane was confirmed by Fourier Transform Infrared Spectroscopy (FTIR), which highlighted the principal absorption bands of both the NC membrane at 1641, 1276, and 835 cm−1 (NO2) and of GO in the range of 3450 cm−1 (CH2-OH). The SEM analysis underlined the well-dispersed and uniform coverage of NC membrane with GO, which displayed thin spider web morphology. The wettability assay indicated that the NC–GO hybrid membrane exhibited slightly lower hydrophilic behavior, with a water contact angle of 26.7°, compared to the 15° contact angle of the NC control membrane. The NC–GO hybrid membranes were used to separate oligonucleotides that had fewer than 50 nucleotides (nt) from complex solutions. The features of the NC–GO hybrid membranes were tested for extraction periods of 30, 45, and 60 min in three different complex solutions, i.e., an aqueous medium, an α-Minimum Essential Medium (αMEM), and an αMEM supplemented with fetal bovine serum (FBS). The oligonucleotides were desorbed from the surface of the NC–GO hybrid membrane using Tris-HCl buffer with a pH of 8.0. Out of the three media utilized, the best results were achieved after 60 min incubation of the NC–GO membranes in αMEM, as evidenced by the highest fluorescence emission of 294 relative fluorescence units (r.f.u.). This value corresponded to the extraction of approximately 330–370 pg (≈7%) of the total oligo-DNA. This method is an efficient and effortless way to purify short oligonucleotides from complex solutions.

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Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Toader,

Nitu,

Ionita

2023

Molecules

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“…3.4 UV-vis spectrum analysis of the interactions between the AuNFs, AuNSs, AuNRs, and HSA UV-vis absorption spectroscopy is an important method that is used to study the interactions between the AuNPs and HSA (Li et al, 2020). In this work, UV-vis absorption spectroscopy was used to study the interactions between the AuNPs with the three different morphologies and HSA.…”

Section: Tem Detection Of Nanomorphologymentioning

confidence: 99%

Interactions between gold nanoparticles with different morphologies and human serum albumin

Dai,

Chen,

Yin

et al. 2023

Front. Chem.

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Introduction: Three different shapes of gold nanoparticles were synthesized in this experiment. At the same time, studies compared their effects with human serum albumin (HSA).Methods: Gold nanoparticles (AuNPs) with three different morphologies, such as, nanospheres (AuNSs), nanorods (AuNRs), and nanoflowers (AuNFs) were synthesized via a seeding method and their characteristic absorption peaks were detected using ultraviolet-visible (UV-vis) absorption spectroscopy, Telectron microscopy (TEM), Dynamic Light Scattering (DLS) and Zeta potential measurements, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) to study the interactions between them and HSA. By comparing the thermodynamic parameters and quenching mechanism of the three materials, similarities and differences were determined in their interactions with HSA.Results: The results showed that with an increase in the concentration of the AuNPs with the three different morphologies, the UV-vis absorption peak intensity of the mixed solution increased, but its fluorescence intensity was quenched. This indicates that the three types of AuNPs interact with HSA, and that the interactions between them represent a static quenching process, which is consistent with the conclusions derived from three-dimensional fluorescence experiments. Through variable-temperature fluorescence experiments, the binding constants, number of binding sites, and thermodynamic parameters of the interactions between the three types of AuNPs and HSA were determined. The Gibbs free energy changes were <0, indicating that the reactions of the three types of AuNPs with HSA are spontaneous, resulting in associated matter. Binding constant measurements indicated that the strongest binding took place between the AuNFs and HSA. In addition, the results of fluorescence, CD spectroscopy, and FTIR showed that three different shapes of AuNPs can induce conformational changes in HSA and reduce the α-helix content. Among them, AuNFs have the smallest ability to induce conformational changes.Discussion: According to studies, AuNFs interact more favorably with HSA. This can be used as a reference for the administration of drugs containing AuNPs.

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“…Furthermore, the versatile nature of NH 2 -PEG-rGO extends beyond its role as a support material for ssDNA immobilization, encompassing the facile adsorption of ssDNA on the rGO lattice, which involves both covalent and non-covalent interactions, with a particular emphasis on non-covalent pi-stacking interactions as the primary pathway. Conversely, double-stranded (ds) DNA exhibits a weaker binding affinity to the rGO surface compared to ssDNA, primarily because of the electrostatic repulsion from the phosphate backbone [ 33 , 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid

Toader,

Grigorean,

Ionita

2024

Biomolecules

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A hybrid material, consisting of commercially available nitrocellulose (NC) membrane non-covalently modified with amino-polyethylene glycol functionalized reduced graphene oxide (NH2-PEG-rGO) nanoparticles, was successfully synthesized for oligonucleotide extraction. Fourier Transform Infrared Spectroscopy (FTIR) confirmed the modification of the NC membrane, revealing characteristic peaks of both compounds, i.e., NC and NH2-PEG-rGO. Scanning Electron Microscopy (SEM) exhibited morphological changes in the NC/NH2-PEG-rGO hybrid membrane, marked by the introduction of NH2-PEG-rGO particles, resulting in a distinctly smothered surface compared to the porous surface of the NC control membrane. Wettability assays revealed hydrophobic behavior for the NC/NH2-PEG-rGO hybrid membrane, with a water contact angle exceeding 90°, contrasting with the hydrophilic behavior characterized by a 16.7° contact angle in the NC membrane. The performance of the NC/NH2-PEG-rGO hybrid membrane was evaluated for the extraction of ssDNA with fewer than 50 nucleotides from solutions containing various ionic species (MnCl2, MgCl2, and MnCl2/MgCl2). The NC/NH2-PEG-rGO hybrid membrane exhibited optimal performance when incubated in MgCl2, presenting the highest fluorescence emission at 525 relative fluorescence units (r.f.u.). This corresponds to the extraction of approximately 610 pg (≈13%) of the total oligo-DNA, underscoring the efficacy of the pristine material, which extracts 286 pg (≈6%) of oligo-DNA in complex solutions.

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Studies on binding of single‐stranded DNA with reduced graphene oxide–silver nanocomposites

Cited by 6 publications

References 53 publications

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Interactions between gold nanoparticles with different morphologies and human serum albumin

Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid

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