Studies on bovine breda virus

Lamouliatte, F.; Pasquier, Pierre; Rossi, Fiorella; Laporte, J.; Loze, J.P.

doi:10.1016/0378-1135(87)90015-0

Cited by 20 publications

(20 citation statements)

References 10 publications

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“…values of positive and negative control sera among assays performed in different days. Cross-reactivity of PToV with other related viruses infecting pigs has been discarded in the three assays tested (ELISA, Western blot and virus neutralization) and these results confirm that these viruses are serologically unrelated, and are in agreement with previous results described by other authors (Woode et al, 1982(Woode et al, , 1985Lamouliatte et al, 1987). However, CPS antibodies recognized both PToV-N and BEV-N proteins (not shown), indicating that the animals used to obtain the serum had been infected by, or, exposed to PToV.…”

Section: Discussionsupporting

confidence: 89%

Molecular characterization of a new PToV strain. Evolutionary implications

et al. 2009

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Toroviruses are emergent viruses, belonging to the Nidovirales order, that remain mostly ignored, despite they are able to infect different species of domestic animals and humans, causing enteric diseases and diarrhea. Thus far, only five variants of porcine torovirus (PToV) have been identified. In this report we describe the identification and partial characterization of a new strain of porcine torovirus (PToV-BRES) that was detected by RT-PCR in a swine faecal specimen from a farm in Brescia (Italy). The complete genes coding for the nucleocapsid (N), hemagglutinin-esterase (HE) and membrane (M) proteins were amplified, and sequence analysis showed that PToV-BRES is a new PToV strain that, based on the HE gene sequence, is phylogenetically related to P4 strain, that was up to now the only member of a distinct PToV lineage. The nucleocapsid protein from PToV-BRES was expressed in insect cells as a his-tagged protein, purified by affinity chromatography and used to develop an ELISA method to detect antibodies against PToV. This assay was evaluated using a serum collection including 45 samples from three commercial farms from Spain. High antibody prevalence against PToV was observed in the three farms, both in adult animals and in piglets, which could suggest that PToV might be endemic in Spanish porcine population. The ELISA method developed in this work could be useful in future epidemiological surveys about toroviruses.

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Section: Discussionsupporting

confidence: 89%

Molecular characterization of a new PToV strain. Evolutionary implications

et al. 2009

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“…So far, all attempts to grow BRV in vitro have failed (Woode et al, 1982); recent observations by Lamouliatte et al (1987) need to be confirmed. For this reason, only limited studies on the characterization of the virus (Koopmans et al, 1986 ), its diagnosis, pathology and epidemiology (Woode, 1987 ) were done.…”

Section: Introductionmentioning

confidence: 98%

Seroepidemiology of Breda virus in cattle using ELISA

Koopmans

Boom

Woode

et al. 1989

Veterinary Microbiology

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Two direct blocking enzyme linked immunosorbent assays (ELISA) for the detection of antibodies to Breda virus in sera of cattle were compared. An ELISA with consecutive addition of antigen and test serum to an antibody-coated plate gave higher positive: negative absorbence ratios than an ELISA in which antigen and test serum were added simultaneously. Sera collected from breeding and fattening herds in The Netherlands (n = 1313) and the F.R.G. (n = 716) were tested, and antibodies to Breda virus were demonstrated in 94% of adult cattle. Ninety percent of newborn calves had high levels of maternal antibodies, which waned until the age of 3 months. Active seroconversion occurred between 7 and 24 months in most animals.

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“…33 These animal rotavirus strains possess a high degree of genetic homology with human strains, but animal -to -human transmission is uncommon because most animal rotaviruses are species specifi c. 34 Human toroviruses associated with gastroenteritis in immunocompromised patients serologically resembles bovine (Breda virus) 35 and equine (Berne virus) 36 toroviruses. 33 These animal rotavirus strains possess a high degree of genetic homology with human strains, but animal -to -human transmission is uncommon because most animal rotaviruses are species specifi c. 34 Human toroviruses associated with gastroenteritis in immunocompromised patients serologically resembles bovine (Breda virus) 35 and equine (Berne virus) 36 toroviruses.…”

Section: Animalmentioning

confidence: 99%