Studies on cell growth stimulating substances of low molecular weight. Part 3. Resorcinin, a mammalian cell growth stimulating substance produced by Cytophaga johnsonae.

Imai, Shinsuke; Fujioka, Kana; Furihata, Kazuo; Fudo, Ryosuke; Yamanaka, Shigeru; Seto, Haruo

doi:10.7164/antibiotics.46.1319

Cited by 19 publications

(19 citation statements)

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“…Additionally, resorstatin (1) has been described as a growth stimulator for mammalian cells. [21] The simultaneous production of flexirubin and resorstatin exhibiting antagonistic activities, might indicate that DAR natural products play a role in the modulation of eukaryotic host cells, an idea supported by other known activities of these compounds. [11,13,22,23] Thus the isolation of compounds corresponding to the identified biosynthesis gene clusters must be a future goal in order to assign their biological functions.…”

Section: Methodsmentioning

confidence: 99%

Formation of 1,3‐Cyclohexanediones and Resorcinols Catalyzed by a Widely Occuring Ketosynthase

et al. 2013

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Section: Methodsmentioning

confidence: 99%

Formation of 1,3‐Cyclohexanediones and Resorcinols Catalyzed by a Widely Occuring Ketosynthase

et al. 2013

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“…Many other genes in this region are predicted to encode enzymes involved in lipid synthesis, such as a (3-oxoacyl)-acyl carrier protein synthase (Fjoh_1087), beta-ketoacyl synthases (Fjoh_1088, Fjoh_1093, Fjoh_1106), and a beta-hydroxyacyl-(acyl carrier protein) dehydratase (Fjoh_1081), and some of these enzymes could have roles in flexirubin synthesis. darA, darB, and perhaps other genes in this region may also be involved in the synthesis of the mammalian cell growth-promoting dialkylresorcinol resorcinin, which is produced by strains of F. johnsoniae (38). Cosmid complementation of the spontaneous flexirubin-negative mutant F. johnsoniae UW102-154 (19,37) confirmed that genes in this region other than darA and darB are involved in flexirubin synthesis, since the complementing plasmid, pMM340, carried a region spanning Fjoh_1078 to Fjoh_1089 (Fig.…”

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confidence: 99%

Novel Features of the Polysaccharide-Digesting Gliding Bacterium Flavobacterium johnsoniae as Revealed by Genome Sequence Analysis

McBride

Xie

Martens

et al. 2009

Appl Environ Microbiol

218

253

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The 6.10-Mb genome sequence of the aerobic chitin-digesting gliding bacterium Flavobacterium johnsoniae (phylum Bacteroidetes) is presented. F. johnsoniae is a model organism for studies of bacteroidete gliding motility, gene regulation, and biochemistry. The mechanism of F. johnsoniae gliding is novel, and genome analysis confirms that it does not involve well-studied motility organelles, such as flagella or type IV pili. The motility machinery is composed of Gld proteins in the cell envelope that are thought to comprise the "motor" and SprB, which is thought to function as a cell surface adhesin that is propelled by the motor. Analysis of the genome identified genes related to sprB that may encode alternative adhesins used for movement over different surfaces. Comparative genome analysis revealed that some of the gld and spr genes are found in nongliding bacteroidetes and may encode components of a novel protein secretion system. F. johnsoniae digests proteins, and 125 predicted peptidases were identified. F. johnsoniae also digests numerous polysaccharides, and 138 glycoside hydrolases, 9 polysaccharide lyases, and 17 carbohydrate esterases were predicted. The unexpected ability of F. johnsoniae to digest hemicelluloses, such as xylans, mannans, and xyloglucans, was predicted based on the genome analysis and confirmed experimentally. Numerous predicted cell surface proteins related to Bacteroides thetaiotaomicron SusC and SusD, which are likely involved in binding of oligosaccharides and transport across the outer membrane, were also identified. Genes required for synthesis of the novel outer membrane flexirubin pigments were identified by a combination of genome analysis and genetic experiments. Genes predicted to encode components of a multienzyme nonribosomal peptide synthetase were identified, as were novel aspects of gene regulation. The availability of techniques for genetic manipulation allows rapid exploration of the features identified for the polysaccharide-digesting gliding bacteroidete F. johnsoniae.

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“…Resorcinin (6) was isolated from Cytophaga johnsoniae AJ No. 12589 and was found to stimulate the growth of NIH 3T3 mouse fibroblasts cells (Imai et al 1993). DB-2073 (1) was isolated from Pseudomonas sp.…”

Section: Biological Activitymentioning

confidence: 99%

“…Other known and described examples of DARs and related compounds are stemphol (3) (Stodola et al 1973), resorstatin (4) (Kato et al 1993), microcarbonin A (5) (Beresovsky et al 2006), resorcinin (6) (Imai et al 1993), isopropylstilbene (IPS) (7) (Joyce et al 2008), and its derivative lumiquinone (Fig. 1).…”

Section: Introductionmentioning

confidence: 98%

Biosynthesis and function of bacterial dialkylresorcinol compounds

Schöner

Kresovic

Bode

2015

Appl Microbiol Biotechnol

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This review summarizes the research of bacterially produced dialkylresorcinols (DARs). We will give an overview about the DAR-related research during the last 40 years. Furthermore, a brief introduction into the class of ketosynthases (KS) and examples of these enzymes which show a deviation to the usual catalytic activity is given. One of these is DarB, which is involved in the DAR biosynthesis. The clustering and distribution of the DAR biosynthesis gene clusters (BGC), that has been identified in more than 100 genomes from taxonomically distinct bacteria, is discussed regarding the structures of the biosynthetic products from these BGCs. Finally, the biological activities of described DARs are summarized and possible methods for the detection and structure elucidation of DARs are given.

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Studies on cell growth stimulating substances of low molecular weight. Part 3. Resorcinin, a mammalian cell growth stimulating substance produced by Cytophaga johnsonae.

Cited by 19 publications

References 0 publications

Formation of 1,3‐Cyclohexanediones and Resorcinols Catalyzed by a Widely Occuring Ketosynthase

Formation of 1,3‐Cyclohexanediones and Resorcinols Catalyzed by a Widely Occuring Ketosynthase

Novel Features of the Polysaccharide-Digesting Gliding Bacterium Flavobacterium johnsoniae as Revealed by Genome Sequence Analysis

Biosynthesis and function of bacterial dialkylresorcinol compounds

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