Studies on DNA-recipient interaction I. Investigation of the interaction between recipients and transfection stimulators with fluorescent probes

Sabelnikov, Alexander G; Moiseeva, T. F.; Avdeeva, A.V.; Il'yashenko, B. N.

doi:10.1016/0005-2787(74)90251-2

Cited by 4 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noteworthy that transfection of protaminetreated spheroplasts with A and T4 DNAs was also completely insensitive to inhibitory concentrations of potassium cyanide or even greatly increased (in rec+ and recA cells) in its presence (38). The interaction of Ca2`and protamine with E. coli cell membranes in the course of competence induction results in considerable structural changes of the latter as evidenced by the fluorescent probe technique (29,31). These changes, in the case of Ca2 , seem to be unique, since neither Mg2+, Tris at 00C, nor EDTA can induce a similar effect (29).…”

Section: Discussionmentioning

confidence: 99%

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Sabelnikov¹,

Domaradsky²

1981

J Bacteriol

View full text Add to dashboard Cite

The effect of various metabolic inhibitors (carbonylcyanid-m-chlorophenylhydrazone, nigericin, valinomycin, dicyclocarbodliimide, arsenate, NaF, etc.) and lipid-soluble synthetic ions (tetraphenylphosphonium bromide and tetraphenylboron sodium) on deoxyribonucleic acid (DNA) entry during transformation of Ca2+-treated Escherichia coli cells with plasmid DNA and on cell viability was investigated. In contrast to intact cells, Ca2+-treated E. coli cells were permeable to nigericin, valinomycin, and the other drugs tested. The inhibitors differentially affected [14C]proline active transport, and whereas some drugs inhibited transformation, the effects did not correlate with the effects on transport. The most potent inhibitors of transformation were nigericin, dicyclocarbodiimide, and tetraphenylboron sodium. Carbonylcyanid-m-chlorophenylhydrazone, tetraphenylphosphonium bromide, and valinomycin were relatively inactive. Tetraphenylboron sodium-and nigericin-treated cells bound more plasmid [14C]DNA in the deoxyribonuclease-resistant form than the control and other sample cells. Nevertheless, the penetration of exogenous plasmid DNA into the cell was greatly reduced, at least in case of nigericin. Unlike the other drugs, nigericin and dicyclocarbodiimide drastically affected the cell viability, the former within very short times of interaction. It is concluded that proton motive force does not play any significant role in DNA entry into Ca2+-treated E. coli cells. The results also suggest that adenosine 5'-triphosphate is not required for DNA entry either. The inhibitory effect of certain drugs is discussed in terms of structural perturbations induced by the drugs in cell envelope membranes. the presence of the uncoupler CCCP, Ca2+treated cells do remain permeable to exogenous DNAs and some DNA-binding antibiotics, even at 370C, whereas nornally they lose permeability. These data, although suggestive, cannot give an unambiguous answer as to the nature of the energy requirement during exogenous DNA up-435

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Sabelnikov¹,

Domaradsky²

1981

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Thus the second hypothesis may also be correct; protamine sulfate in the periplsmic space of competent spheroplasts forms a complex with transfecting DNA and facilitates irreversible uptake of DNA (320). To support this hypothesis, protamine sulfate was coupled to a fluorescent probe and the amount of polymer bound to spheroplasts was measured (321). Protamine sulfate was found associated with spheroplasts, but no measurements were made with noncompetent cells.…”

Section: Lysozyme-edta Technique-mechanismmentioning

confidence: 99%

Transfection of Enterobacteriaceae and its applications.

Benzinger¹

1978

Microbiological Reviews

View full text Add to dashboard Cite

Interaction between isolated DNA of phage λ and Escherichia coli spheroplasts treated with sturine

Moiseeva¹,

Dityatkin²,

Sabelnikov³

et al. 1977

Bull Exp Biol Med

View full text Add to dashboard Cite

Studies on DNA-recipient interaction I. Investigation of the interaction between recipients and transfection stimulators with fluorescent probes

Cited by 4 publications

References 16 publications

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Effect of metabolic inhibitors on entry of exogenous deoxyribonucleic acid into Ca2+-treated Escherichia coli cells

Transfection of Enterobacteriaceae and its applications.

Interaction between isolated DNA of phage λ and Escherichia coli spheroplasts treated with sturine

Contact Info

Product

Resources

About