Studies on enhanced post-illumination respiration in microalgae

Beardall, John; Burger-Wiersma, Tineke; Rijkeboer, Machteld; Sukenik, Assaf; Lemoalle, Jacques; Dubinsky, Zvy; Fontvielle, D.

doi:10.1093/plankt/16.10.1401

Cited by 61 publications

(33 citation statements)

References 9 publications

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“…This is in contrast to the earlier work of Falkowski et al (1985b) and Beardall et al (1994) that showed a marked increase in respiration rate in freeliving phytoplankton following exposure to high light.…”

Section: Discussioncontrasting

confidence: 99%

Diurnal hysteresis in coral photosynthesis

Levy

Dubinsky

Schneider

et al. 2004

Mar. Ecol. Prog. Ser.

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In oxygenic photosynthesizing organisms, it has been noticed on a number of occasions that photosynthetic performance was lower in the afternoon than in the morning, at the same light intensities. This hysteresis phenomenon is called the 'afternoon depression' and has been observed in phytoplankton, macroalgae, and higher plants. Here we characterize, with high temporal resolution, in situ diel courses of oxygen evolution and chlorophyll fluorescence yields in 3 Indo-Pacific corals (Favia favus, Goniopora lobata, Plerogyra sinuosa) and 2 Caribbean symbiotic corals (Montastrae faveolata and Porites astreoides) using a 3-chamber submersible respirometer and a SCUBAbased fast repetition rate fluorometer. In contrast to all previously published cases, the oxygen measurements revealed an unexpected hysteresis, with higher photosynthetic rates occurring in the afternoon than in the morning. The oxygen diel patterns were highly consistent in all organisms examined, including the 3 corals and 2 macroalgae (Ulva sp. and Gracilaria sp.) from the Red Sea. Surprisingly, the diurnal patterns of the quantum yields of photochemistry in Photosystem II (PSII), assessed from variable fluorescence (quantum yield of photochemistry in PSII measured under ambient light, ∆F '/F m ') exhibited much higher variability and often showed a hysteresis pattern opposite to that of oxygen. In most organisms the values of ∆F '/F m ' and the deduced rates of photosynthetic electron transport were higher in the morning than in the afternoon; however, the opposite trend with lower values of ∆F '/F m ' in the morning was also observed. Lower values of ∆F '/F m ' were always accompanied by higher quantum yields of non-photochemical quenching, consistent with the energetic balance within the primary photosynthetic reactions. The direction of the diurnal hysteresis in variable fluorescence appears to be species-specific and may vary even within the same species, reflecting microscale variability in bio-optical properties and gross photosynthesis of the corals.

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Section: Discussioncontrasting

confidence: 99%

Diurnal hysteresis in coral photosynthesis

Levy

Dubinsky

Schneider

et al. 2004

Mar. Ecol. Prog. Ser.

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“…Not including this probability in the calculation of gross oxygen production, as was the case in this study, may result in consistent underestimation of gross oxygen production rates and consequently of a(),. Experimental evidence for increases in mitochondrial respiration rate in the light has been found for a number of Chl ccontaining algae (Grande et al 1989;Weger et al 1989;Daneri et al 1992;Beardall et al 1994). The higher mitochondrial respiration rates in the light are probably due to increased substrate supply by photosynthesis.…”

Section: Discussionmentioning

confidence: 94%

Light dependence of quantum yields for PSII charge separation and oxygen evolution in eucaryotic algae

Flameling

Kromkamp

1998

Limnology & Oceanography

126

105

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Quantum yields of photosystcm I1 (PSII) charge separation (@& and oxygen production ((I) (,,) were determined by simultaneous measurements of oxygen production and variable fluorescence in four different aquatic microalgae representing three different taxonomic groups: the freshwater alga Scenedesmus protuberans (Chlorophyceae) and the marine algae Phneorystis globosa (Prymnesiophyceae), Emiliania huxleyi (Prymnesiophyceae), and Phaeodactylum tricornutum (Bacillariophyceae). In S. protuberans, P. tricornutum, and E. huxleyi, light-dependent variability was observed in the ratio of a(,,, to a,,, i.c. in the number of oxygen molecules produced per electron generated by PSII. The ratio ao,:@,, was highly variable at low light intensities (E < OSE,), and at higher light intensities (E > OSE,) @(,, : @,, showed a nonlinear decrease with increasing light intensity. In contrast, in P. globosa. a trend in a<,, : @,, could not be distinguished, and this species showed a decrease in @'o, : $ during the day, indicating a dependency of a(,, : a,, on light history. Additionally, considerable interspecific quantitative differences in a(,, : a,, were observed. Two possible interpretations to explain the variability in '.I+)! : a, are discussed. Assuming that 0, is a reliable measure of the quantum yield for charge separation at PSII, one interpretation is that net oxygen production is influenced by processes that consume oxygen or affect linear electron transport (e.g. cyclic electron transport around PSII, pseudocyclic electron transport in the Mehler reaction, Rubisco oxygenase activity, and lightdependent mitochondrial respiration). A second interpretation, however, suggests that at saturating light, changes in photosynthesis turnover time occur, such that $ does not predict the steady-state O2 yield.Quantum yields of phytoplankton photosynthesis are usually defined as the quantum yields for O? production (CacjL) or C fixation (@c,,,) (Kok 1948;Myers 1980;Babin et al. 1996). Because measurements of @oz and @'co, are laborious and time-consuming, much attention has been focused lately on the use of variable chlorophyll fluorescence as a tool to measure the quantum yield of charge separation in photosystem II (PSIl) reaction centers (Genty et al. 1989). This technique is rapid and noninvasive and may offer high temporal and spatial resolution when used in field measurements (Schreiber et al. 1986). Theoretically, the rate of noncyclic photosynthetic electron transport of a PSI1 (J) can be calculated from the quantum yield of PSI1 charge separation (a,,) according to J = @,J%s,,rwhere E is the incident light intensity and cr,,,, is the absorption cross section of PSII, which determines the fraction of the incident light intensity that is actually used by PSI1 (e.g. Kolber and Falkowski 1993; Kroon 1991; Hofstraat et al. 1994, Biehler andFock 1995). In order to use J as a measure for photosynthesis, the relationships between @,,, @coL, and @coz have to be well established. According to the stoichiometry of the Z-schem...

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“…Enhanced post-illumination respiration (EPIR) was first reported by Falkowski et al (1985) and reexamined by Beardall et al (1994). However, since that phenomenon that by steeply increasing respiration losses reduces net production and yields is dose dependent, the brevity of the high light exposure minimizes its effects.…”

Section: Mechanismsmentioning

confidence: 99%