Studies on Fructan Accumulation in Wheat (Triticum aestivum L.). II. Changes in degree of polymerization of fructan under treatment at 1.DEG.C. in dark.

Yukawa, Tomoyuki; Watanabe, Yoshiaki; Yamamoto, Shinsuke

doi:10.1626/jcs.63.430

Cited by 8 publications

(5 citation statements)

References 8 publications

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“…5). It was previously demonstrated that fructan breakdown is induced in leaves kept in the dark at 1 -C (Yukawa et al, 1994). No 6&1-FEH w1 expression was found in young leaves or roots grown at warmer temperatures (not shown), suggesting that cold might be important for expression of the gene.…”

Section: Putative Role(s) Of 6and1-feh W1mentioning

confidence: 90%

“…A large amount of graminan-type fructan accumulates in vegetative wheat tissues during the period of cold acclimation and fructan content is associated with freezing tolerance, over-wintering ability and snow mold resistance (Yukawa et al, 1994;Yoshida et al, 1998). Sucrose: sucrose 1-fructosyltransferase (1-SST) and sucrose: fructan 6-fructosyltransferase (6-SFT) are key enzymes for fructan biosynthesis in Poaceae but the role of fructan: fructan 1-fructosyltransferase (1-FFT) is less clear (Kawakami and Yoshida, 2002;Nagaraj et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose

Kawakami

Yoshida

Ende

2005

Gene

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Section: Putative Role(s) Of 6and1-feh W1mentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose

Kawakami

Yoshida

Ende

2005

Gene

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“…This depolymerization process has been extensively studied in chicory (Van den Ende and Van Laere, 2007, and references therein). Both fructan contents and FEH activities have also been measured in wheat (Yukawa et al, 1994(Yukawa et al, , 1995Yoshida et al, 1998;Van den Ende et al, 2003). Both fructans and FEHs were found in the apoplast (Livingston and Henson, 1998;Van den Ende et al, 2005a), leading to the hypothesis that apoplastic fructans could be trimmed by these FEHs, and thus generate a mixture of oligofructans, sucrose, and fructose that might be optimal for stabilization of the plasma membrane.…”

Section: Feh Activity On Fructans Generates Low Dp Sugars Sucrose and Hexosesmentioning

confidence: 99%

Plant fructans in stress environments: emerging concepts and future prospects

Valluru

Ende

2008

Journal of Experimental Botany

342

218

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Plants are sessile and sensitive organisms known to possess various regulatory mechanisms for defending themselves under stress environments. Fructans are fructose-based polymers synthesized from sucrose by fructosyltransferases (FTs). They have been increasingly recognized as protective agents against abiotic stresses. Using model membranes, numerous in vitro studies have demonstrated that fructans can stabilize membranes by direct H-bonding to the phosphate and choline groups of membrane lipids, resulting in a reduced water outflow from the dry membranes. Inulin-type fructans are flexible random-coiled structures that can adopt many conformations, allowing them to insert deeply within the membranes. The devitrification temperature (T(g)) can be adjusted by their varying molecular weights. In addition, above T(g) their low crystallization rates ensure prolonged membrane protection. Supporting, in vivo studies with transgenic plants expressing FTs showed fructan accumulation and an associated improvement in freezing and/or chilling tolerance. The water-soluble nature of fructans may allow their rapid adaptation as cryoprotectants in order to give optimal membrane protection. One of the emerging concepts for delivering vacuolar fructans to the extracellular space for protecting the plasma membrane is vesicle-mediated, tonoplast-derived exocytosis. It should, however, be noted that natural stress tolerance is a very complex process that cannot be explained by the action of a single molecule or mechanism.

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“…5a), suggests that these tissues may depend mainly on fructan degradation as energy source. It has previously been demonstrated that fructan breakdown is induced in leaves kept in the dark at 1°C (Yukawa et al ., 1994). In contrast to other FEHs, it is tempting to speculate that the 6‐KEH gene is not inhibited but rather induced by a certain Suc concentration or Suc flux.…”

mentioning

confidence: 99%

Cloning, characterization and functional analysis of novel 6‐kestose exohydrolases (6‐KEHs) from wheat (Triticum aestivum)

et al. 2005

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Summary• Cereals accumulate graminan-type fructans which are subject to stress-related degradation by fructan 1-exohydrolases (1-FEHs) and fructan 6-exohydrolases (6-FEHs). To find new FEH genes related to freezing tolerance, a cold-hardened wheat crown cDNA library was screened.• Here we report the cloning, purification and characterization of two novel 6-kestosidase (6-KEH) isoenzymes from wheat crowns ( Triticum aestivum ). Functional characterization in Pichia pastoris confirmed the extreme substrate selectivity for the fructan trisaccharide 6-kestose.• Northern blotting showed that 6-KEH transcripts were constantly detected at the same level from autumn to winter in crown but not in leaf tissues. Apoplastic fluid isolations and activity measurements strongly suggest that 6-KEH is localized in the apoplast.• It is proposed that 6-KEHs, together with other FEHs, might be involved in the breakdown of apoplastic fructans which may fulfil a role as membrane protectors under stress. Alternatively, a role in signalling processes, or in the degradation of exogenous 6-kestose from bacterial origin, cannot be excluded.

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Studies on Fructan Accumulation in Wheat (Triticum aestivum L.). II. Changes in degree of polymerization of fructan under treatment at 1.DEG.C. in dark.

Cited by 8 publications

References 8 publications

Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose

Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcose

Plant fructans in stress environments: emerging concepts and future prospects

Cloning, characterization and functional analysis of novel 6‐kestose exohydrolases (6‐KEHs) from wheat (Triticum aestivum)

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