Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci

Swanson, John

doi:10.1128/iai.19.1.320-331.1978

Cited by 244 publications

(164 citation statements)

References 13 publications

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“…Neisseria gonorrhoeae outer nnembrane contains a class of proteins called opacity (Opa, Pll) proteins. The expression of these proteins contributes to colony opacity and to intracellular aggregation (Swanson, 1978). Opa protein expression also correlates with growth of gonococci in vivo\ Opa^ organisms predominate in some anatomical sites whereas Opa" variants abound in others (James and Swanson, 1978;Draper ef a/., 1980;Swanson etal., 1988).…”

Section: Introductionmentioning

confidence: 99%

The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes

Bhat

Gibbs

Barrera

et al. 1991

Molecular Microbiology

165

176

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Variants of Neisseria gonorrhoeae MS11 show distinct colony morphologies because of the expression of a class of surface components called opacity (Opa, PII) proteins. Southern analyses combined with molecular cloning of genomic DNA from a single variant of MS11 has identified 11 opa genes contained in separate loci. These opa genes code for distinct opacity proteins which are distinguishable at their variable domains. The opa gene analyses were also extended to divergent variants of MS11. These studies have shown that, during in vitro and in vivo culture, 10 of the 11 opa genes did not undergo significant change in their primary sequence. However, in these variants, one gene (opaE) underwent non-reciprocal inter-opa recombinations to generate newer Opa variants. Phylogenic analysis of the opa gene sequences suggests that the opa gene family have evolved by a combination of gene duplication, gene replacement and partial inter-opa recombination events.

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Section: Introductionmentioning

confidence: 99%

The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes

Bhat

Gibbs

Barrera

et al. 1991

Molecular Microbiology

165

176

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“…Bacteria N. gonorrhoeae strain FA19 was used throughout this study. Non-pilated, non-opaque (Opa") bacteria were maintained by daily passage on clear typing media (Swanson, 1978) and harvested by swabbing bacteria from 18-h cultures and resuspending them in Dulbecco's phosphate-buffered saline, pH7.4 (DPBS) as previously described (Judd, 1982).…”

Section: Methodsmentioning

confidence: 99%

Isolation of the periplasm of Neisseria gonorrhoeae

Judd

Porcella

1993

Molecular Microbiology

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The periplasm of Neisseria gonorrhoeae should be similar to other Gram-negative bacteria, but no published reports confirm this assumption. We used a periplasmic isolation procedure developed in Escherichia coli to release the periplasmic contents of N. gonorrhoeae. The resultant periplasmic extract lacked lipopolysaccharide, protein markers of inner or outer membranes, surface-radiolabelled protein components, or ribosomal proteins. The periplasmic extract contained a single haem protein believed to be a c-type cytochrome known to exist in the periplasm of other Gram-negative species, and retained significant alkaline phosphatase activity. The dominant protein species released in the periplasmic extract was the gonococcal homologue of elongation factor Tu, a major component released in similar periplasmic extracts of E. coli. These data showed that the extraction procedure selectively released periplasmic components and that the gonococcal periplasm was comparable to that of E. coli. Further analysis of the gonococcal periplasm may provide important insights into the physiology of this pathogen of humans.

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“…In addition to the swimmer-swarmer cell dimorphism, V. parahaemolyticus exhibits another kind of phenotypic switching, described as the opaque-translucent variation in colony morphology. It was postulated that differences in colony structure or packing result in differential light transmission [156]. The opaR gene, encoding a transcriptional regulatory protein homologous to LuxR of V. harveyi, controls opacity [154] and is involved in capsular polysaccharide production [157].…”

Section: Opar the V Harveyi Luxr-homologue Negatively Regulates Swmentioning

confidence: 99%

Quorum sensing and swarming migration in bacteria

2004

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Bacterial cells can produce and sense signal molecules, allowing the whole population to initiate a concerted action once a critical concentration (corresponding to a particular population density) of the signal has been reached, a phenomenon known as quorum sensing. One of the possible quorum sensing-regulated phenotypes is swarming, a flagella-driven movement of differentiated swarmer cells (hyperflagellated, elongated, multinucleated) by which bacteria can spread as a biofilm over a surface. The glycolipid or lipopeptide biosurfactants thereby produced function as wetting agent by reducing the surface tension. Quorum sensing systems are almost always integrated into other regulatory circuits. This effectively expands the range of environmental signals that influence target gene expression beyond population density. In this review, we first discuss the regulation of AHL-mediated surface migration and the involvement of other low-molecular-mass signal molecules (such as the furanosyl borate diester AI-2) in biosurfactant production of different bacteria. In addition, population density-dependent regulation of swarmer cell differentiation is reviewed. Also, several examples of interspecies signalling are reported. Different signal molecules either produced by bacteria (such as other AHLs and diketopiperazines) or excreted by plants (such as furanones, plant signal mimics) might influence the quorum sensing-regulated swarming behaviour in bacteria different from the producer. On the other hand, specific bacteria can reduce the local available concentration of signal molecules produced by others. In the last part, the role and regulation of a surface-associated movement in biofilm formation is discussed. Here we also describe how quorum sensing may disperse existing biofilms and control the interaction between bacteria and higher organisms (such as the Rhizobium-bean symbiosis).

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Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci

Cited by 244 publications

References 13 publications

The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes

The opacity proteins of Neisseria gonorrhoeae strain MS11 are encoded by a family of 11 complete genes

Isolation of the periplasm of Neisseria gonorrhoeae

Quorum sensing and swarming migration in bacteria

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