Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: Comparison of its distribution in normal, benign and malignant prostates

Doctor, Vatsala M.; Sheth, A. R.; Simha, M M; Arbatti, N.J.; Aaveri, J P; Sheth, Nandini A.

doi:10.1038/bjc.1986.85

Cited by 68 publications

(44 citation statements)

References 12 publications

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“…There is a high concentration of prostatic inhibinlike peptide (PIP) in the normal and hyperplastic human prostate [4, 23, 301. Biologically active rat PIP has been shown by in vivo [lo] as well as in vitro studies [20].…”

Section: Introductionmentioning

confidence: 99%

“…High levels of PIP observed in the prostate-rich secretions of human seminal plasma (HSP) have further substantiated its prostatic origin [ 11. The importance of PIP as a prostatic tissue marker [4] and in confirming the prostatic origin of difficult-to-diagnose invasive tumors in metastic lesions [25] has been demonstrated by immunocytochemical localization studies. These data led us to study the status of immunoreactive PIP in the rat ventral and dorsal prostate and its modulation by steroid and protein hormones.…”

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confidence: 99%

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Modulation of Rat Prostatic Inhibinlike Peptide (PIP) by Steroids and Protein Hormones

Teni

Sheth

1988

Archives of Andrology

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Prostatic inhibinlike peptide (PIP) was detected in the ventral but not in the dorsal lobe of rat prostate.On orchiectomy, PIP concentration in the ventral prostate increased significantly, whereas it decreased on testosterone administration and attained value comparable with that in intact control. Estradiol-170-treated animals exhibited high levels of PIP in absence of significant alterations in the protein content. The effect of prolactin and human chorionic gonadotropin on PIP concentration was not so prominent at the dose levels studied. The present study thus demonstrates negative regulation of PIP by testosterone and stimulatory effect of estradiol-170 on PIP in rat ventral prostrate. INTRODUCTIONThere is a high concentration of prostatic inhibinlike peptide (PIP) in the normal and hyperplastic human prostate [4, 23, 301. Biologically active rat PIP has been shown by in vivo [lo] as well as in vitro studies [20]. High levels of PIP observed in the prostate-rich secretions of human seminal plasma (HSP) have further substantiated its prostatic origin [ 11. The importance of PIP as a prostatic tissue marker [4] and in confirming the prostatic origin of difficult-to-diagnose invasive tumors in metastic lesions [25] has been demonstrated by immunocytochemical localization studies. These data led us to study the status of immunoreactive PIP in the rat ventral and dorsal prostate and its modulation by steroid and protein hormones. MATERIALS AND METHODSSteroid and protein hormones used for the study were procured from Sigma Chemicals. Ninety-dayold male rats of Sprague Dawley strain were divided into the following groups: group I, intact controls; group 11, effect of castration; group 111, in vivo effect of steroid hormones: (a) testosterone (T) (500 pg), subgroup contained a minimum of five rats. All the animals except the intact controls were bilaterally castrated. Twenty-four hours after castration, the animals in groups I11 and IV were treated daily with the above-mentioned doses of respective hormones, administered subcutaneously for six days. Three hours after the last injection, animals of all the groups were killed and prostates excised. The dorsal and ventral lobes were weighed separately and homogenized individually in 0.01 M PBS, pH 7.0, and centrifuged at 800 g for 30 min at 4 "C. The supernatants were stored at -20 "C until analyzed for protein by Lowry's Method [14] and PIP by radioimmunoassay (RIA) [29]. A homologous preparation of PIP isolated from HSP [26] was used as a reference standard and as an antigen for radioiodination using Iz5I, obtained from Radiochemical Centre, Amersham, U. K. Iodination was carried out according to the method of Greenwood et al.[6] with appropriate modifications [29]. The specific activity of the labeled hormone ranged from 100 to 150 pCiIpg PIP. Specific antibodies to PIP were raised in rabbit by active immunization. Details regarding the high specificity of this antiserum and RIA methodology have been described [22]. The sensitivity of the assay was 0.7 to 40 ng o...

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Modulation of Rat Prostatic Inhibinlike Peptide (PIP) by Steroids and Protein Hormones

Teni

Sheth

1988

Archives of Andrology

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“…11,12 As described in an recent review, 13 several groups have used a variety of approaches in both tissue and serum samples to demonstrate decreasing levels of MSMB in prostate cancer compared with normal controls, prompting the suggestion that MSMB may be a promising biomarker for prostate cancer. [14][15][16][17][18][19] Additionally, MSMB located at chromosome 10q11.2 has recently been reported as an important candidate gene for prostate cancer susceptibility. 20,21 Several causal risk alleles affecting the level of gene transcription have been identified in the region upstream of the coding sequence.…”

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Evaluation of the prognostic significance of MSMB and CRISP3 in prostate cancer using automated image analysis

et al. 2011

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Despite prostate cancer being the most frequent cancer in men in the Western world, tissue biomarkers for predicting disease recurrence after surgery have not been incorporated into clinical practice. Our group has previously identified b-microseminoprotein (MSMB) and cysteine-rich secretory protein-3 (CRISP3) as independent predictors of biochemical recurrence after radical prostatectomy. The purpose of the present study was to use automated image analysis, enabling quantitative determination of MSMB and CRISP3 expressions in a large cohort and to validate the previous findings. MSMB and CRISP3 protein expressions were assessed on tissue microarrays constructed from 3268 radical prostatectomy specimens. Whole-slide digital images were captured, and a novel cytoplasmic algorithm was used to develop a quantitative scoring model for cytoplasmic staining. Classification regression tree analysis was used to group patients, with different risk for biochemical recurrence, depending on level of protein expression. Patients with tumors expressing high levels of MSMB had a significantly reduced risk for biochemical recurrence after radical prostatectomy (HR ¼ 0.468; 95% CI 0.394-0.556; Po0.001). Multivariate analysis adjusted for clinicopathological parameters revealed that MSMB expression was an independent predictor of decreased risk of recurrence (HR ¼ 0.710; Po0.001). We found no correlation between CRISP3 expression and biochemical recurrence. In this current study, we applied a novel image analysis on a large independent cohort and successfully verified that MSMB is a strong independent factor, predicting favorable outcome after radical prostatectomy for localized prostate cancer.

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“…Although a clear function for PSP94 has not been identified, its reduced expression in prostate cancer suggests it to be a prognostic indicator in prostate carcinoma , Doctor et al 1986, Brar et al 1988, Hyakutake et al 1993, Liu et al 1993, Kammer et al 1993. It has also been suggested as an apoptosis-based anti-tumor agent for hormone refractory prostate cancer (Garde et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Prostatic secretory protein (PSP94) expression in human female reproductive tissues, breast and in endometrial cancer cell lines

Baijal-Gupta¹,

Clarke²,

Finkelman³

et al. 2000

Journal of Endocrinology

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PSP94 ( microseminoprotein,MSP) is one of the three major proteins secreted by the normal human prostate gland. Using reverse transcriptase polymerase chain reaction (RT-PCR) and Southern blotting, PSP94 transcripts were shown in human endometrium, myometrium, ovary, breast, placenta and in the human endometrial cancer cell lines KLE and AN3 CA. Primers used in these studies were specific for human prostate PSP94, and were derived from its flanking non-coding regions. The results were confirmed by sequence analysis of two independently derived clones from normal human breast tissues and the other two from KLE cells respectively. The sequences were identical with the coding sequence of human prostate PSP94 cDNA. Using RNA from the endometrial tissues, two different transcripts of 487 bp, equivalent to prostate PSP94 and 381 bp, corresponding to prostate PSP57, its alternately spliced form, were amplified by RT-PCR. Human ovary, breast, placenta and endometrial cancer cell lines (KLE, AN3 CA), however, showed only the full length, 487 bp, PSP94 transcript. We further demonstrated by in situ hybridization that PSP94 mRNA is expressed specifically in the glandular epithelial cells, and not in the stroma of both the human endometrial and breast tissues. Further, using image analysis of in situ hybridization data, the levels of PSP94 mRNA in the cycling endometrial tissues and in breast confirmed the differential levels of expression in the cycling endometrium (P<0·005). This study distinctly demonstrated significant expression of PSP94 mRNA in human uterine, breast and other female reproductive tissues as well in the endometrial cancer cell lines, suggesting that it may have a role in these tissues as a local autocrine paracrine factor.

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Studies on immunocytochemical localization of inhibin-like material in human prostatic tissue: Comparison of its distribution in normal, benign and malignant prostates

Cited by 68 publications

References 12 publications

Modulation of Rat Prostatic Inhibinlike Peptide (PIP) by Steroids and Protein Hormones

Modulation of Rat Prostatic Inhibinlike Peptide (PIP) by Steroids and Protein Hormones

Evaluation of the prognostic significance of MSMB and CRISP3 in prostate cancer using automated image analysis

Prostatic secretory protein (PSP94) expression in human female reproductive tissues, breast and in endometrial cancer cell lines

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