Studies on in Vitro Multiplication of Carnations Iv. The Multiplication and Rooting of Shoots

Effects of an auxin, cytokinins, and genotype on in vitro propagation of Impatiens sp. 'T63-1' and 'Starfire'

“…can be rapidly propagated in vitro by an exogenous hormonal regulation. Such rapid clonal propagation using shoot-tips or meristems has been previously reported for other herbaceous ornamental plants such as carnations Langhans 1975 andDabiski et al 1979), Chrysanthemum spp. (Grewal and Sharma 1978), Pelargonium spp.…”

“…1984). Some researchers reported that an interaction between NAA and cytokinin showed better effects on root formation than NAA itself (Rao et al 1973, Kunisaki 1975, Brandao and Salema 1977, and Dabiski et al 1979. We observed that 'Starfire' produced abnormal roots which were very short and thick in sane treatments of experiment II, while 'T63-1'…”

“…He also stated that the best photoperiod for a given tissue culture is dependent upon the light used, and probably other factors. Many workers have commomly used 14 to 18 hr photoperiods for tissue cultures (Styer and Chin 1983, Dabiski et al 1979, Kerbauy 1984, Stoltz 1984, Blazich and Novitzky 1984, Stephens et ..Q.l. 1985, Ettinger and Preece 1985, and Econanou and Spanoudaki 1985.…”

“…NAA combined with a cytokinin is more often used for callus production rather than NAA by itself. Many researchers have found NAA and cytokinin effects on callus production for several herbaceous ornamental plants (Dabiski et al 1979, Sharma and Mitra 1976, Brandao and Salema 1977, Bilkey and Mccown 1979, Banta and Torres 1984, and Torres and Natarella 1984. Explant diameter seems to affect shoot multiplication and shoot elongation.…”

“…There are two opposite reports for the effect of NAA on callus versus shoot elongation. Dabiski et al (1979) reported that the addition of NAA to the medium stimulated callus and shoot elongation, while Earle and Langhans (1975) reported that NAA stimulated callus growth but suppressed shoot elongation in carnation.…”