Studies on in Vitro Propagation Methods in Cactus Species of the Genera Melocactus, Cereus and Lobivia

Monostori, Tamás; Tanács, Lajos; Mile, Lajos

doi:10.17660/actahortic.2012.937.31

Cited by 7 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These morphotypes showed a particular development, and their presence in greenhouse's seedlings had not been detected previously. The appearance of different morphological shoot types in cacti grown under in vitro conditions have been also reported by other authors [13,[68][69][70], and it is relatively frequent. Although the exact mechanism is still unknown, it seems that the epigenetic factors responsible for regulating gene expression could be behind the appearance of these morphological variants [71].…”

Section: Descriptions Of Morphotypessupporting

confidence: 75%

Micropropagation and Acclimatization of Gymnocalycium cv. Fancy (Cactaceae): Developmental Responses to Different Explant Types and Hormone Conditions

Cortés-Olmos,

Guerra-Sandoval,

Blanca-Giménez

et al. 2023

Plants

View full text Add to dashboard Cite

The Gymnocalycium genus includes numerous highly valued species in the ornamental plant market and their propagation is usually carried out using traditional methods. However, there is a lack of information regarding the efficiency of micropropagation through in vitro tissue culture techniques on these species. So, with the objective of establishing an efficient micropropagation protocol that allows for optimizing the plant obtaining processes, the morphogenic potential of Gymnocalycium cv. Fancy has been studied in this work. For this purpose, plants of two different sizes (medium and large) were used as the starting material, from which three types of explants were obtained (apex, central discs and bases). The effect of three plant growth regulators (6-Benzylaminopurine, BAP; Kinetin, KIN; and Thidiazuron, TDZ) at three different concentrations each were studied, and the number of generated shoots, the frequency of appearance of callogenesis and rhizogenesis by explant and condition were assessed. An efficient protocol based on the use of KIN at 4 µM and central discs as a starting explant was developed. Moreover, the obtained sprouts rooted successfully (especially using BAP at 2 µM), and their subsequent acclimatization was very effective. Furthermore, emergence of a new morphotype is presented, that has not previously been described.

show abstract

Section: Descriptions Of Morphotypessupporting

confidence: 75%

Micropropagation and Acclimatization of Gymnocalycium cv. Fancy (Cactaceae): Developmental Responses to Different Explant Types and Hormone Conditions

Cortés-Olmos,

Guerra-Sandoval,

Blanca-Giménez

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…This research showed that even in the absence of hormones, the shoot could stretch to its maximum length. Even at high BAP concentrations, vitrification could not be seen on axillary shoots (Monostori, Tanács, & Mile, 2010). However, this research found that a greater concentration of BAP may enhance shoot elongation more than a lower concentration of BAP.…”

Section: Effect Different Plant Pgrs On Shoot Lengthcontrasting

confidence: 57%

Effect of Plant Growth Regulators, Basal Media Strength and Carbon Sources on Hylocereus Costaricensis (Red Dragon Fruit) Seed Germination

2021

EAJSE

View full text Add to dashboard Cite

Hylocereus costaricensis, commonly known as red dragon fruit or 'pitaya,' is famous for its purple-red flesh. It has achieved international recognition as an ornamental plant and secondly as a commercial fruit crop. Containing high level of vitamin C and antioxidants like betalans, hydroxycinnamates and flavonoids that includes edible fibre with numerous health benefits including weight loss, improve digestion, lower LDL cholesterol, and boost immune system. However, there is no large-scale production of dragon fruit because the seed storage viability is not reliable; the present study aims to employ tissue culture techniques supplied with different plant growth regulators (PGRs), the strength of growth media, and carbon sources. Seed germination was determined by growing the seeds in media enriched with benzyl aminopurine (BAP) 1 mg/L, 2 mg/L BAP, and 15 mg/L gibberellins (GA). Then, the seed growth was measured under different strengths of Murashige and Skoog (MS) basal media, which are full strength, 1/2, 1/4 and 1/8 strength, that promoted the seed germination the most. The percentage of seed germination was determined, and the effects of seed germination was measured by the period of seed to germinate, shoot length, and root length of the plants. In this study, all the dragon fruit seeds were successfully germinated. The seed shows the higher germination is MS + 2 mg/L BAP within 2 days, but the elongation of the shoot is not the fastest. The seed germination is effective in 1/8 media strength on day 2; the seeds are already germinated. However, the elongation for roots and shoots is the best in 1/2 media strength. The effect of carbon sources influences the fastest day to germinate, demonstrated in sucrose 30 g/L but not for root and shoot length. The 30 g/L glucose enhances the root length the highest, which is 2.48 cm. The average shoot length shows in TCS0 and TCS3 was similar, which was 1.38 cm. The effect of carbon sources that boost the shoot length the most is in TCS3 (2.04 cm), fructose 15 g/L. Those factors should be considered to enhance the best condition for dragon fruit planting and achieve the large-scale production,

show abstract

“…The reduction in salt concentration, more precisely ammonium (NH 4 )/nitrate (NO 3 ) ratio, is also effective to decrease shoot hyperhydricity in T. mombergeri , and a similar finding was also reported in Cereus jamacaru . For instance, vitrified and abnormal shoots of C. jamacaru recovered with the reduction in basal salt, vitamins, and sugar concentrations (Monostori et al, 2010 ). The increase in calcium amount (double-dose) also prevented the hyperhydration of regenerated shoots (Santos-Díaz et al, 2022 ).…”

Section: Constraints Of In Vitro Propagation In Cactimentioning

confidence: 99%

Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review

et al. 2022

View full text Add to dashboard Cite

Cacti are one of the most significant and diversified groups of angiosperms, distributed and cultivated globally, mostly in semi-arid, arid, and the Mediterranean climate regions. Conventionally, they are propagated by seeds or through vegetative propagation via rooted offshoots or grafting. However, these multiplication procedures remain insufficient for mass propagation. In vitro culture techniques are utilized to mass propagate endangered and commercial cacti species. These include somatic embryogenesis and plant regeneration through indirect or direct organogenesis. The latter is a promising tool for commercial clonal propagation of high-value species and has been successfully implemented for several species, such as Mammillaria, Hylocereus, Cereus, Echinocereus, and Ariocarpus. However, its success depends on explant type, basal nutrient formulation of culture medium, and types and concentrations of plant growth regulators. This study aimed to assess the potential of in vitro propagation methods applied to cacti species and discuss the different factors affecting the success of these methods. This study has also highlighted the insufficient work on Opuntia species for mass propagation through axillary buds' proliferation. The development of an efficient micropropagation protocol is thus needed to meet the supply of increasing demand of Opuntia species for human consumption as fruit, animal feed, and ecological restoration in semi-arid and arid zones.

show abstract

Studies on in Vitro Propagation Methods in Cactus Species of the Genera Melocactus, Cereus and Lobivia

Cited by 7 publications

References 8 publications

Micropropagation and Acclimatization of Gymnocalycium cv. Fancy (Cactaceae): Developmental Responses to Different Explant Types and Hormone Conditions

Micropropagation and Acclimatization of Gymnocalycium cv. Fancy (Cactaceae): Developmental Responses to Different Explant Types and Hormone Conditions

Effect of Plant Growth Regulators, Basal Media Strength and Carbon Sources on Hylocereus Costaricensis (Red Dragon Fruit) Seed Germination

Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review

Contact Info

Product

Resources

About