Studies on Interaction of Thiamine or Its Related Compounds With Proteins

Effects of Thiamine Propyl Disulfide on Tryptophan Pyrrolase in Rats

“…The results also suggested that TPD effect was responsible for the presence of S-S bond in the molecule, because thiamine did not replace TPD with respect to the activation of the enzyme. These results were consistent with the report that difficulty of binding between thiamine molecule and SH protein was clarified (23).…”

“…As was published by the re ports (3,24), binding activity of propylmercapto group of TPD to bovine serum albumin was found in vitro experiments to increase markedly at pH 8-9. Moreover, the TPD effect suggested that possibility that propylmercapto group combined with SH groups on TP caused an increase in the enzyme activity.…”

Studies on Interaction of Thiamine or Its Related Compounds With Proteins

“…The sulfhydryl group of protein was shown to be involved in the reaction (1,2), the complex formation was proportional to the reaction time (2), and the reaction obeyed a pseudo-first-order reac tion, Arrhenius' equation being applicable (2). From these findings, the product was assumed to be a protein-thiamine complex involving S-S-linkage.…”

Interaction of Thiol-Type Thiamines With Serum Albumin

“…It is possible that the thiamine disulfide (produced by air oxidation during incubation for a long period) reacts with the SH groups of serum albumin to pros duce bound thiamine (6,7). Therefore, several studies were made to differentiate the reaction of thiamine as in Equation a from that of thiamine disulfide (Table 3) .…”