Studies on Metabolism and Disposition of Sizofiran (SPG), an Anti-tumor Polysaccharide. I. : Excretion and Tissue Distribution of &lt;SUP&gt;14&lt;/SUP&gt;C-SPG

Horiba, Masahiro; Akima, Kazuo; Hase, Toyoji; Arika, Tadashi; Amemiya, Kouji; Munechika, Kazuo; Tabata, Kengo; Aimoto, Tachio

doi:10.1248/yakushi1947.108.8_763

Cited by 3 publications

(3 citation statements)

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“…This suggests that the excretion might be dose-dependent . 63 The excretion in the rat urine is in the form of low molecular weight metabolites (< 10 KDa).74 A similar excretion pattern has been found for lentinan26 and the excretion of methyl-lentinan was similar to that of the unsubstituted poly~accharide.~~ Compared with pure beta-linked immunomodulator polysaccharides, the complex derivatives are metabolized to a greater degree in mammals. For example, after the oral administration of PS-K to mice 70 per cent of the dose was excreted in the expired air, 20 per cent in the faeces and 10 per cent in the urine over the first 24 h.31…”

Section: Metabolism and Excretionmentioning

confidence: 73%

“…74 The complex polysaccharide, biostim, is partially degraded crossing the duodenal villi, nevertheless it appears to retain at least some of its antigenic ability after oral a b~o r p t i o n .~~ PS-K is broken down and rapidly excreted in the expired air as carbon dioxide in mice31 while schizophyllan is, as we have already seen, eliminated very slowly in the urine in rats. 63,65 In rats over a 6-month period, 41 per cent of administered activity is recovered in the urine and 8 per cent in the faeces at a dose level of 0.2 mg kg-l while at a dose of 2 mg kg-' the figures are 26 and 2 per cent respectively. This suggests that the excretion might be dose-dependent .…”

Section: Metabolism and Excretionmentioning

confidence: 99%

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Immunomodulator polysaccharides: Chemistry, disposition and metabolism

Trnovec

Hrmová

1993

Biopharm & Drug Disp

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Section: Metabolism and Excretionmentioning

confidence: 73%

Section: Metabolism and Excretionmentioning

confidence: 99%

Immunomodulator polysaccharides: Chemistry, disposition and metabolism

Trnovec

Hrmová

1993

Biopharm & Drug Disp

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“…The structural change of bupleuran 2llc in the liver after oral administration remains to be investigated. For the study of pharmacokinetics of pharmacologically active polysaccharides, fluorescence-(19) or radio-labelled polysaccharide (20) has been used in order to chase the polysaccharide in the body. However, there are problems that the uniform labeling of the probe to the polysaccharide is very…”

Section: Detection Of Orally Administrated Acidic Polysaccha Ride Framentioning

confidence: 99%

Detection and Tissue Distribution of Anti-Ulcer Pectic Polysaccharides fromBupleurum falcatumby a Polyclonal Antibody

Sakurai¹,

Matsumoto²,

Kiyohara³

et al. 1996

Planta Med

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Anti-sera against the "ramified" region (PG-1) of an anti-ulcer polysaccharide (bupleuran 2IIc), which was purified from the roots of Bupleurum falcatum L, were obtained by immunization of rabbits, and a polyclonal anti-bupleuran 2IIc/PG-1-antibody of the IgG class was purified by Protein G and "ramified" region (PG-1) immobilized affinity chromatographies. The antigenic specificity of anti-bupleuran 2IIc/PG-1-IgG was examined by a two-site sandwich ELISA which was developed as an improved method for microanalysis of bupleuran 2IIc using a biotinylated antibody. Another pectin from B. falcatum and anti-complementary pectins from Angelica acutilaba and Glycyrrhiza uralensis also showed significant reactivity to anti-bupleuran 2IIc/PG-1-IgG, although these reactivities were lower than that of bupleuran 2IIc. Other polysaccharides tested such as apple pectin, araban, yeast mannan, pullulan, etc., had negligible reactivity. The KDO-containing region and oligogalacturonides, which were obtained by endo-alpha-(1-->4)-polygalacturonase digestion of bupleuran 2IIc, were also not significantly recognized by anti-bupleuran 2IIc/PG-1-IgG. When bupleuran 2IIc was administered to the mice i.v., the polysaccharide disappeared from the circulation within 24 h and was mainly detected in the liver by the two-site sandwich ELISA. However the clearance of bupleuran 2IIc from the circulation was delayed by pretreatment with iota-carrageenan. When the crude polysaccharide fraction (BR-2), containing mainly bupleurans 2IIb and 2IIc from B.falcatum, was administrated orally to the mice, the polysaccharides were detected in the liver and Peyer's patch.

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