Studies on plasma membranes I. Chemical composition and enzyme content of plasma membranes isolated from rat liver

Emmelot, P.; Bos, Cécile; Benedetti, E.L.; Ruemke, P

doi:10.1016/0304-4165(64)90125-4

Cited by 761 publications

(178 citation statements)

References 29 publications

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“…2 shows that at 0 hr N 74% of the 5'-nucleotidase and ca 64% of the NADPH-cytochrome c reductase activity remain in the final supernate 3 presumably as soluble proteins or protein complexes. This distribution is quite different from that found in liver cell fractions in which NADPH-cytochrome c reductase is strictly a microsomal membrane enzyme (4,19,20), whereas 5'-nucleotidase is associated with membranes in both the plasmalemmal and microsomal fractions (5,9,(20)(21)(22)(23) .…”

Section: Effects Of Aging On Enzyme Activity and Distributioncontrasting

confidence: 67%

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Composition of Cellular Membranes in the Pancreas of the Guinea Pig

Meldolesi

Jamieson

Palade

1971

The Journal of Cell Biology

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A comparative study of the enzymic activities of membrane fractions derived from guinea pig pancreatic homogenates has yielded the following results: Rough microsomal membranes (derived from the rough ER) have the reductase activities of the two microsomal electron transport systems but lack enzyme activities of Golgi-type (TPPase) and plasmalemmal-type (5'-nucleotidase, ß-leucyl naphthylamidase, Mg-ATPase). Smooth microsomal membranes (derived primarily from the Golgi complex), zymogen granule membranes, and plasmalemmal fractions possess overlapping enzyme activities of plasmalemmal type, in different relative concentrations for each fraction. In addition, the smooth microsomal membranes exhibit TPPase and ADPase activity and share with rough microsomes the reductase activities of the two electron transport chains. Taken together with recent data on the lipid composition of the same fractions (2), these results indicate that the membranes of the pancreatic exocrine cell are chemically and functionally distinct, and hence do not mix with one another during the transport of secretory products.

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Section: Effects Of Aging On Enzyme Activity and Distributioncontrasting

confidence: 67%

“…Ranges are given in italics . (5,20,22,28,29) . As shown in Table I, both rough and smooth microsomes have NADH-and NADPH-cytochrome c reductase activities ; their SA p I is higher in rough microsomes, although with the correction mentioned the activities of the two fractions become nearly equal .…”

Section: Enzyme Activities In Pancreatic Cell Fractionsmentioning

confidence: 99%

Composition of Cellular Membranes in the Pancreas of the Guinea Pig

Meldolesi

Jamieson

Palade

1971

The Journal of Cell Biology

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“…4). are clearly observed as reported by Neville (14) and Emmelot et al (15). When plasma membranes were incubated with 10 111 of CC14 at 0 C or at 37 C, these structures became ob scure and the adhesion of small particles having high electron density was observed (Fig.…”

Section: Resultssupporting

confidence: 86%

STUDIES ON THE FUNCTION OF CELL MEMBRANE 10th REPORT :EFFECTS OF CCl4 ON THE MARKER ENZYME ACTIVITIES AND FINE STRUCTURES OF RAT LIVER PLASMA MEMBRANES AND MICROSOMES IN VITRO

Masuda

Yano

Sumida

et al. 1975

Japanese Journal of Pharmacology

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“…Milk fractions including MFGM prepared from several samples of milk by this procedure were assayed for 5'-nucleotidase [4] , nucleotide pyrophosphatase [6] and ATPases [8] …”

Section: Methodsmentioning

confidence: 99%