Studies on Preparation and Characterization of Blend Polymers for Hydrogels Synthesis and Use for Protein Release

Abstract

Carboxy methyl cellulose, pectin, chitosan or acrylic acid-poly (vinyl alcohol) blend hydrogels were prepared using Glutaraldehyde (GLU) as chemical or sodium hexameta phosphate (SHMP) as physical cross-linking agent. The prepared hydrogels have carried the advantage properties of both blend materials and can be used for loading and releasing of BSA protein. The degree of swelling of the prepared hydrogels was measured due to their different functional groups in different pHs of (pH4, pH7 and pH9) in addition to the electrolyte solution of 0.1 N NaCl. The blend hydrogels are characterized for their chemical structure using FT-IR. The XRD analysis was investigated for determination of the hydrogels crystallographic structure. The differential thermal analysis DTA and scanning electron microscope SEM were depended to study the thermal stability and surface morphology of the hydrogel respectively. The hydrogels were loaded with BSA protein model, and time of loading at (1.5, 3.0, 4.5 and 6.0) hrs were measured, beside the pH of the loading solution of ph4, pH6 and pH8 were considered and the BSA concentrations of (0.5, 1.0, 1.5 and 2.0) g/L have been investigated for maximum loading percentages %L max of BSA on the hydrogels. Finally the loaded BSA was released under different release conditions in 0.9% w/vNaCl solution and pH4, pH6 and pH8 were depended and the BSA was tested to release at 15, 25 and 40 o C releasing medium temperatures.

Blended Natural Support Materials—Collagen Based Hydrogels Used in Biomedicine

“…In order to obtain improved mechanical properties, greater pore sizes collagen hydrogels need to be synthesized at lower gelation temperatures due to the formation of fewer, longer, and thicker collagen fibrils important characteristics in drug delivery systems and wound healing. In tissue engineering application mimicking the specific morphology and manufacturing the 3D structures of cell are important parameters which need to be controlled using different methods: Realize the gelation process in customized micromolds or bioprinting technology [ 36 , 37 , 38 , 39 ].…”

Controlled Delivery System for NPK Agrochemical Release from Chitosan Copolymer Hydrogels

“…Poly (Vinyl Alcohol) (PVA), is a hydrophilic synthetic polymer that has a semi-crystalline structure. PVA has a good ability to blend with other polymers and form hydrogel as described properties (Jabrail and K Al-Ojar, 2019). Carboxymethyl Cellulose (CMC), the anionic water-soluble polysaccharides material is an important cellulose derivative that has linear and long chains (Jabrail and K Al-Ojar, 2019).…”