Studies on Regenerating Adrenal Cortex 2. Autoradiographic and Electron Microscopic Observations

Seki, Masatoshi; Sekiyama, Shigetaka; Miyahara, Hiroko; Ichii, Shogo

doi:10.1507/endocrj1954.16.361

Cited by 16 publications

(5 citation statements)

References 5 publications

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“…A close correlation between the structure of mitochondrial crystae and the steroidogenic function in rat adrenals was also reported (Seki et al, 1969). The rate of 3H-leucine incorporation into the outer membrane fraction was higher than that into the inner membrane fraction, however there might be a possibility that the outer membrane fraction was contaminated with microsomes.…”

Section: Resultssupporting

confidence: 60%

Short-and Long-Term Effect of ACTH in vivo on the Incorporation of 3H-Leucine into the Submitochondrial Fractions from Rat Adrenal Glands

1970

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SynopsisShort-(3.5hr) and long-term (3 days) effects of ACTH in vivo on the incorporation of 3H-leucine into cell fractions from rat adrenal glands were examined, with special regard to effects of the hormone on the rate of the amino acid incorporation into submitochondrial fractions. An injection of ACTH caused a significant increase in the rate of the radioactive amino acid incorporation into the inner membrane and soluble matrix fractions of adrenal mitochondria within 3.5hr while no significant effect of ACTH administration was observed in the other subcellular fractions. Injections of ACTH for consecutive 3 days resulted in the elevated rate of the incorporation into all subcellular fractions except into the acid-soluble fraction. Physiological significance of the results obtained was discussed with respect to the steroidogenic effect of ACTH.

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Section: Resultssupporting

confidence: 60%

Short-and Long-Term Effect of ACTH in vivo on the Incorporation of 3H-Leucine into the Submitochondrial Fractions from Rat Adrenal Glands

1970

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“…Previously, by using autoradiographic techniques, we assumed that adrenal regeneration in the autotransplant process took place at the graft periphery and proceeded from subcapsular glomerulosa cells (Vendeira et al, 1992). In addition, autoradiographic studies using the gland enucleation model showed a rapid proliferation before the end of the first month, with mitotic cells located in a few cell layers of the subcapsular region (Seki et al, 1969). This result suggested a preponderant role for the classical adrenoglomerulotrophic factors (ACTH, angiotensin 11, and K + ) in stimulating the mitotic activity of zona glomerulosa (Nussdorfer, 1986).…”

Section: Discussionmentioning

confidence: 99%

Modulation of autotransplanted adrenal gland by endothelin‐1: A morphological and biochemical study

et al. 1996

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“…A selective stimulation of 3H-leucine incorporation into the inner membrane fraction of rat adrenal mitochondria was observed after an acute administration of ACTH (Ichii et al, 1970), therefore it might at first be thought that some particular protein synthesis is required for the reorganization of membraneous structure of mitochondria and this in turn reflects the increased rate of steroid hormone biosynthesis in the gland, since changes in the morphological structure of mitochondrial crystae under the regulatory influence of ACTH were observed (Kahri, 1968; Seki et al, 1969). Furthermore, mitochondria' crystae are recognized as a site of ACTH action (Yago andIchii, 1969, Yago et al, 1970).…”

Section: Discussionmentioning

confidence: 99%

Effect of ACTH on Turnover of Phospholipids in Rat Adrenal Glands

et al. 1971

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Time-course studies of 3H-choline incorporation into adrenal phospholipids revealed that the specific radioactivity reached to a maximum level at 9 hr after the isotope injection in untreated control animals, while in ACTH-treated animals, a maximum incorporation was already attained by 2 hr postinjection. The effect of ACTH on the incorporation was relatively prompt, the effect became evident as early as 1.5 hr after the treatment of animals. The rate of the incorporation in ACTH-treated animals was about twice that of the control. The incorporated radioactivity was decreased with the half-life of 2.9 days in phospholipids of control adrenals. On the other hand, the incorporated choline was well retained in adrenals of animals which receive daily treatment with ACTH throughout the experimental period of 7 days. The rate of phospholipid exchange between liver microsomes and adrenal mitochondria was not markedly influenced by treatment of animals with ACTH. Physiological implications of these observations were discussed.

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Studies on Regenerating Adrenal Cortex 2. Autoradiographic and Electron Microscopic Observations

Cited by 16 publications

References 5 publications

Short-and Long-Term Effect of ACTH <I>in vivo</I> on the Incorporation of <SUP>3</SUP>H-Leucine into the Submitochondrial Fractions from Rat Adrenal Glands

Short-and Long-Term Effect of ACTH <I>in vivo</I> on the Incorporation of <SUP>3</SUP>H-Leucine into the Submitochondrial Fractions from Rat Adrenal Glands

Modulation of autotransplanted adrenal gland by endothelin‐1: A morphological and biochemical study

Effect of ACTH on Turnover of Phospholipids in Rat Adrenal Glands

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