Studies on RNA metabolism in the nerve cells of Hippocampus during training in rats

Cupello, A.; Hydén, Holger

doi:10.1007/bf00235811

Cited by 16 publications

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References 21 publications

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“…Most eukaryotic mRNAs and hnRNAs (heterogeneous nuclear RNA) contain sequences of polyadenylic acid about 200 nucleotides-long at the 3'-OH terminus (Edmonds and Caramela, 1969;Lim and Canellakis, 1970; Edmonds et al, 1971; Nakazato a n d E d m o n d s , 1972; DeLarco and Guroff, 1972; B a n k s a n d J o h n s o n , 1973; Hemminki, 1974;L i m et al, 1974;Mahony and Brown, 1975;Bondy et al, 1977;Cupello and Hyden, 1978). Poly(A)-containing RNA (poIy(A)+ RNA) can be selectively bound to oligo(dT)-cellulose; thus, the presence of such p o l y ( A ) sequences in most eukaryotic mRNA provides a method for its identification as well as a means for its isolation.…”

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Studies on Polyadenylic Acid‐Containing RNA from Developing Nervous System of the Chicken the

Murphy

Mezei

1980

Journal of Neurochemistry

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To investigate certain biochemical aspects of myelination, a study was undertaken of the messenger‐like RNA in the nervous system of pre‐ myelinating 14‐day embryos and of myelinating 17‐day embryos and 3‐day chicks. The central and peripheral nervous systems of the chick were found to contain and to actively synthesize poly(A)+ RNA. RNA species binding to oligo(dT)‐cellulose contained a relatively high proportion of adenylate residues and were resistant to the actions of pancreatic and T1 ribonucleases. Preparations labeled by incubation with adenosine in vitro showed a decrease in the proportion of poly(A)+ RNA as the age of the animal increased, while preparations labeled in vivo exhibited the opposite trend. Polyacrylamide gel electrophoretograms of both in vivo and in vitro labeled pqeparations showed that the poly(A)+ fractions contained mainly heterodisperse RNA species. The average molecular size of poly(A)+ RNAs of purified polysomal fractions of nerve RNA from 3‐day chicks was smaller than 18S, whereas that of total poly(A)− RNA was larger than 18s. The proportion of poly(A)+ molecules larger than 18s was lower in the rapidly myelinating nerve tissues of 17‐day embryos and post‐hatching chicks than in those of premyelinating 14‐day embryos. Similar results were obtained for crude nuclear RNA fractions or RNA preparations fractionated under denaturing conditions. These results are consistent with previous work showing that the embryonic peripheral nerve contains a larger proportion of high‐molecular‐weight, messenger‐like RNA molecules than does nerve tissue from young chicks or adults.

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confidence: 99%

Studies on Polyadenylic Acid‐Containing RNA from Developing Nervous System of the Chicken the

Murphy

Mezei

1980

Journal of Neurochemistry

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“…In addition to the task difficulty, the bimanual use in the present task might also elevate cognitive demand. It has been reported that rats show handedness, i.e., a preference to use the right forepaw [ 20 ], and that reversal of handedness activates the hippocampus [ 21 ]. The dependency of hippocampal theta oscillations on task difficulty is like that reported in FM theta [ 22 ].…”

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Change in hippocampal theta oscillation associated with multiple lever presses in a bimanual two-lever choice task for robot control in rats

et al. 2018

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Hippocampal theta oscillations have been implicated in working memory and attentional process, which might be useful for the brain-machine interface (BMI). To further elucidate the properties of the hippocampal theta oscillations that can be used in BMI, we investigated hippocampal theta oscillations during a two-lever choice task. During the task body-restrained rats were trained with a food reward to move an e-puck robot towards them by pressing the correct lever, ipsilateral to the robot several times, using the ipsilateral forelimb. The robot carried food and moved along a semicircle track set in front of the rat. We demonstrated that the power of hippocampal theta oscillations gradually increased during a 6-s preparatory period before the start of multiple lever pressing, irrespective of whether the correct lever choice or forelimb side were used. In addition, there was a significant difference in the theta power after the first choice, between correct and incorrect trials. During the correct trials the theta power was highest during the first lever-releasing period, whereas in the incorrect trials it occurred during the second correct lever-pressing period. We also analyzed the hippocampal theta oscillations at the termination of multiple lever pressing during the correct trials. Irrespective of whether the correct forelimb side was used, the power of hippocampal theta oscillations gradually decreased with the termination of multiple lever pressing. The frequency of theta oscillation also demonstrated an increase and decrease, before and after multiple lever pressing, respectively. There was a transient increase in frequency after the first lever press during the incorrect trials, while no such increase was observed during the correct trials. These results suggested that hippocampal theta oscillations reflect some aspects of preparatory and cognitive neural activities during the robot controlling task, which could be used for BMI.

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Labeling of poly(A) associated RNA in synaptosomes and the other subcellular fractions of rat cerebral cortex in basal conditions and during training

Cupello¹,

Hydén

1982

J of Neuroscience Research

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The labeling of total and poly(A) associated RNA from the subcellular fractions of cerebral cortex was studied after a 1-hour 3H-uridine pulse in control and trained rats. In the control animals, the fraction of newly synthesized brain cortex RNA which is found in synaptosomes is the minimal one. However, in synaptosomes the proportion of poly(A)+ RNA within newly synthesized RNA is maximal. Training of the rats does not influence total incorporation of label into RNA in the brain cortex and the distribution of newly synthesized RNA within the subcellular fractions. The proportions of poly(A)+ RNA within total labeled RNA in the various fractions are also unaffected.

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Studies on RNA metabolism in the nerve cells of Hippocampus during training in rats

Cited by 16 publications

References 21 publications

Studies on Polyadenylic Acid‐Containing RNA from Developing Nervous System of the Chicken the

Studies on Polyadenylic Acid‐Containing RNA from Developing Nervous System of the Chicken the

Change in hippocampal theta oscillation associated with multiple lever presses in a bimanual two-lever choice task for robot control in rats

Labeling of poly(A) associated RNA in synaptosomes and the other subcellular fractions of rat cerebral cortex in basal conditions and during training

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