Studies on temperature-dependent ultraviolet light-sensitive mutants of bacteriophage T4: The structural gene for T4 endonuclease V

Sato, Kousei; Sekiguchi, Mutsuo

doi:10.1016/0022-2836(76)90071-1

Cited by 28 publications

(11 citation statements)

References 20 publications

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“…The total measurement would include all three. Since v-induced excision repair appears to occur before replicative synthesis (26), one might expect the increased UV sensitivity of T4v DNA synthesis, as has been observed here and elsewhere (8). On the other hand, if excision repair is expanded in the absence of x-y recombinational repair, one might expect a decreased radiosensitivity of DNA synthesis for the x and y mutants, as was shown above.…”

Section: Resultssupporting

confidence: 57%

Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis

Melamede¹,

Wallace²

1977

J Virol

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The bacteriophage T4 recombination-deficient mutants x and y exhibited decreased rates of DNA synthesis as compared to wild-type T4. Mutant-induced DNA synthesis was more sensitive to mitomycin C than was wild-type synthesis. However, DNA synthesis in mutant-and wild-type-infected cells exhibited the same sensitivity to UV light and X-irradiation. When high-specific-activity label was administered at various times postinfection, mutant DNA synthesis resembled that of wild type for 12 min, after which time mutant-induced incorporation was greatly decreased and sensitive to mitomycin C as compared to that of the wild type. Rifampin and chloramphenicol studies indicated that the gene products necessary for synthesis measured at 15 min postinfection, including those of x+ and y+, were transcribed within 2 min and translated within 8 min postinfection. Administration of chloramphenicol to mutant xor mutant y-infected cells exactly 8 min postinfection, however, allowed for increased synthesis at 15 min that was sensitive to mitomycin C. Cells coinfected with T4+ and T4x or T4x and T4y retained a reduced mutant-type synthesis, whereas cells coinfected with T4+ and T4y exhibited a synthesis more closely resembling that of wild type.

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Section: Resultssupporting

confidence: 57%

Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis

Melamede¹,

Wallace²

1977

J Virol

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“…1968) . Further work with temperature-sensitive mutants of this gene has demonstrated that denV codes for a structural, rather than a regulatory, gene product (Sato and Sekiguchi 1976) . Appropriate T4 mutants have also helped to elucidate how both the glycosylase and endonuclease enzymatic functions are associated at the genetic level .…”

Section: Prokaryotic Systems 1 Bacteriophage T4mentioning

confidence: 99%

The Molecular Genetics of the Incision Step in the DNA Excision Repair Process

Rubin

1988

International Journal of Radiation Biology

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This review describes the evolution of research into the genetic basis of how different organisms use the process of excision repair to recognize and remove lesions from their cellular DNA. One particular aspect of excision repair, DNA incision, and how it is controlled at the genetic level in bacteriophage, bacteria, S. cerevisae, D. melanogaster, rodent cells and humans is examined. In phage T4, DNA is incised by a DNA glycosylase-AP endonuclease that is coded for by the denV gene. In E. coli, the products of three genes, uvrA, uvrB and uvrC, are required to form the UVRABC excinuclease that cleaves DNA and releases a fragment 12-13 nucleotides long containing the site of damage. In S. cerevisiae, genes complementing five mutants of the RAD3 epistasis group, rad1, rad2, rad3, rad4 and rad10 have been cloned and analyzed. Rodent cells sensitive to a variety of mutagenic agents and deficient in excision repair are being used in molecular studies to identify and clone human repair genes (e.g. ERCC1) capable of complementing mammalian repair defects. Most studies of the human system, however, have been done with cells isolated from patients suffering from the repair defective, cancer-prone disorder, xeroderma pigmentosum, and these cells are now beginning to be characterized at the molecular level. Studies such as these that provide a greater understanding of the genetic basis of DNA repair should also offer new insights into other cellular processes, including genetic recombination, differentiation, mutagenesis, carcinogenesis and aging.

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“…H. Wunderli and E. Kellenberger (personal communication) have observed DNA to be associated with proheads containing uncleaved p23, and C. L. Hsiao and L. W. Black (personal communication) have obtained evidence, using cold-sensitive and heat-sensitive mutants in gene 20, that p20 acts early in the prohead pathway, as is well established, but that it also acts later during DNA packaging. These results prompted us to examine the giants generated by using mutants involved in some aspect of DNA synthesis and packaging (5,17,24,27,29), particularly since DNA in giant phage appears to be the result of recombination (18). Table 2, footnote c) were the greatest observed for petite production.…”

Section: Resultsmentioning

confidence: 99%

Structural aberrations in T-even bacteriophage. IX. Effect of mixed infection on the production of giant bacteriophage

Cummings¹,

Chapman²,

DeLong³

1977

J Virol

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Studies on temperature-dependent ultraviolet light-sensitive mutants of bacteriophage T4: The structural gene for T4 endonuclease V

Cited by 28 publications

References 20 publications

Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis

Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis

The Molecular Genetics of the Incision Step in the DNA Excision Repair Process

Structural aberrations in T-even bacteriophage. IX. Effect of mixed infection on the production of giant bacteriophage

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