Studies on the biological effects of ozone: 5. Evaluation of immunological parameters and tolerability in normal volunteers receiving ambulatory autohaemotherapy

Shyam

2011

IJOO

Disinfectant and antibacterial properties of ozone are utilized in the treatment of nonhealing or ischemic wounds. We present here a case of 59 years old woman with compartment syndrome following surgical treatment of stress fracture of proximal tibia with extensively infected wound and exposed tibia to about 4/5 of its extent. The knee joint was also infected with active pus draining from a medial wound. At presentation the patient had already taken treatment for 15 days in the form of repeated wound debridements and parenteral antibiotics, which failed to heal the wound and she was advised amputation. Topical ozone therapy twice daily and ozone autohemotherapy once daily were given to the patient along with daily dressings and parenteral antibiotics. Within 5 days, the wound was healthy enough for spilt thickness skin graft to provide biological dressing to the exposed tibia bone. Topical ozone therapy was continued for further 5 days till the knee wound healed. On the 15th day, implant removal, intramedullary nailing, and latissimus dorsi pedicle flap were performed. Both the bone and the soft tissue healed without further complications and at 20 months follow-up, the patient was walking independently with minimal disability.

Section: Discussionmentioning

confidence: 81%

Adjuvant combined ozone therapy for extensive wound over tibia

Shyam

2011

IJOO

“…In all the experiments, we used an ozone concentration of 80 µg/ml, corresponding to the maximum concentration within the therapeutic range recommended in vivo [34][35][36][37][38].…”

Section: Ozone Generationmentioning

confidence: 99%

“…While in vivo, AHT enriched with ozone induces a series of effects that might also be indirectly useful in the treatment of infections, such as: immune modulation [42][43][44][45][46][47][48], antioxidant systems activation [49][50][51][52] and improvement of the microcirculation [53][54][55][56].…”

mentioning

confidence: 99%

<i>In Vitro</i> Evaluation of Ozone Activity on Recent Clinically Isolated Bacterial Strains

Tordiglione¹,

Morselli²,

Scarpa³

et al. 2014

AiM

This study aims to evaluate the cozone bactericidal activity in different suspension media (saline, broth and whole blood) at different exposure times. Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, ESBL-positive Escherichia coli, MDR Pseudomonas aeruginosa were suspended in different media. We used a bacterial concentration of 0.2 MF for all experiments, as this concentration is consistent with the results of septic shock blood experiments. We performed ozone insufflations in a "sealed environment". The total number of insuffla-tions for each experiment ranged from one to four. The gas concentration was maintained at 80 mcg/ml. We con-firmed the bactericidal activity of ozone on saline for all the bacterial strains. Experiments in broth revealed no changes in the bacterial growth. Ozone is primarily bactericidal against E. coli and bacteriostatic on P. aerugi-nosa, S. aureus and E. faecalis on whole blood. This study confirms the bactericidal efficacy of topical ozone ap-plications and supports the need for further evaluations of the therapeutic potential of major ozone autohemo-therapy. The results in E. coli promote further investigations of ozone activity on other Enterobacteriaceae and its potential use in the treatment of urinary infections. In general, these results suggest that ozone-therapy might be an alternative therapy to overcome antibiotic resistance.

“…Ozone, an emerging medical drug, is used in disc hernaition because of its property to break down the proteo-glycan bridges in the nucleus pulposus of disc along with an anti-inflammatory action. 5 In this study we aim to assess the effectiveness of ozone nucleolysis in lumbar intervertebral disc herniation. This study is done over a period of three years, wherein 68 individuals with complaints of low backache and radiation pain due to disc herniation were taken as subjects and were treated with ozone injection in the herniated disc followed up for 2 years.…”

mentioning

confidence: 99%

Ozone Nucleolysis in Lumbar Intervertebral Disc Herniation: Non - Randomized Prospective Analysis

Patil¹,

Garampalli²,

Patil³

2015

jemds