Studies on the constituents of Vitex rotundifolia L. fil.

Kondo, Yoshikazu; Sugiyama, Kazuko; Nozoe, Shigeo

doi:10.1248/cpb.34.4829

Cited by 46 publications

(24 citation statements)

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“…The n-hexane fraction (73 g) of the methanol extract (184 g) of Vitex rotundifolia fruits (4 kg) was subjected to Sephadex LH-20 and a silica gel column chromatography repeatedly to afford five compounds, 1 (428 mg), 2 (176 mg), 3 (93 mg), 4 (834 mg) and 5 (637 mg). The spectral data of compounds 1-5 were identical to those reported in the literature (Taguchi, 1976;Kondo et al, 1986;Kang et al, 1994;Hirobe et al, 1997), and their structures were identified as rotundifuran, chrysosplenol D, luteolin, vitexicarpine and artemetin, respectively. Luteolin was freshly dissolved in dimethylsulphoxide (DMSO) and the final concentration of DMSO did not exceed 0.2%.…”

Section: Methodssupporting

confidence: 77%

Effects of luteolin on the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia cells

Kang

Lee

et al. 2002

Phytotherapy Research

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Luteolin, a flavonoid isolated from the fruit of Vitex rotundifolia, has been examined with regard to the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia HL-60 cells. The concentration required for 50% inhibition of the growth after 96 h was 15 +/- 1.1 microM. The mode of cell death induced by luteolin was found to be apoptosis, as judged by the morphologic alteration of the cells and by the detection of DNA fragmentation using agarose gel electrophoresis. The degree of apoptosis was quantified by a sandwich enzyme immunoassay and flow cytometric analysis. These results suggest that luteolin may be used as potential chemopreventive and chemotherapeutic agents.

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Section: Methodssupporting

confidence: 77%

Effects of luteolin on the inhibition of proliferation and induction of apoptosis in human myeloid leukaemia cells

Kang

Lee

et al. 2002

Phytotherapy Research

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“…In previous papers, [3][4][5][6] we reported the isolation and structural elucidation of iridoids, phenylpropanoids, a flavanone, lignanes, a diterpene glycoside and diterpenoids in the MeOH extract of the fruit of V. rotundifolia. As part of the continuing study of this fruit, we now describe the isolation and structural elucidation of ten new labdane-type diterpenes (1-10), along with a known labdane-type diterpene, vitexilactone (11) 7) from the MeOH extract. The MeOH extract of the fruit of Vitex rotundifolia L. was purified by Diaion HP-20, silica gel, Sephadex LH-20 and Chromatorex ODS column chromatography, as well as by HPLC on ODS and silica gel to afford eleven labdane-type diterpenes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11).…”

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confidence: 99%

Ten New Labdane-Type Diterpenes from the Fruit of Vitex rotundifolia.

et al. 2001

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“…1 H-, 13 C-NMR (DEPT) and 2D NMR were recorded on Bruker AVANCE 500 spectrometer. HR-ESI-MS spectrum was obtained on Bruker APEX spectrometer.…”

Section: Methodsmentioning

confidence: 99%

“…4,5 In the course of screening for bioactive constituents from this TCM, we have isolated norlabdane diterpene vitrifolin A, and evaluated its inhibitory activity on NO production in RAW264.7 cells. 10 Continued investigation of the diterpenoid components resulted in the isolation of a new labdane, vitrifolin B (1), and two known ones, vitexlactam A (2) 11 and vitexilactone (3), 12,13 from the fruits of V. rotundifolia ( Fig. 1).…”

Section: 3mentioning

confidence: 99%