Studies on the Development of the Implantation Reaction in the Mouse Uterus: Influence of Actinomycin D

Finn, C. A.; Bredl, J. C. S.

doi:10.1530/jrf.0.0340247

Cited by 41 publications

(12 citation statements)

References 8 publications

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“…As expected, most of the major changes in methionine incorporation into proteins were decreases, but 13 proteins showed increased incorporation with progesterone withdrawal (MacDonald et al, 1983). Initiation of apoptosis induces the synthesis of specific proteins in many systems (Steller, 1995), and apoptosis can be inhibited or delayed by inhibitors of RNA or protein synthesis (Finn and Bredl, 1973). It seems reasonable that several of the 13 proteins showing increased methionine incorporation with progesterone withdrawal could be newly synthesized proteins required for the completion of apoptosis.…”

Section: Discussionsupporting

confidence: 58%

Progesterone withdrawal and RU-486 treatment stimulate apoptosis in specific uterine decidual cells

et al. 1997

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Progesterone secretion is required for the growth and differentiation of endometrial stromal cells to form decidual cells. For many cells where a growth factor supports cell growth and proliferation, withdrawal of the growth factor initiates apoptosis. This study determined the time course and tissue location of apoptosis in deciduomal tissue after withdrawal of progesterone or injection of the antiprogestin, RU-486. Total DNA was isolated from decidual tissues at intervals after experimental treatments and separated electrophoretically. Internucleosomal DNA fragmentation characteristic of apoptosis was measured by quantitating levels of the 200 bp fragment. Apoptotic cells in tissue sections were detected by direct immunoperoxidase detection of digoxigenin-labeled DNA. Decidual apoptosis reached maximal levels at 12 h after withdrawal of progesterone or injection of RU-486. Increased concentrations of apoptotic cells were observed at the periphery of the growing deciduoma and in the antimesometrial deciduoma near the luminal epithelium after both treatments. These results suggest the withdrawal of progestin initiates apoptosis in cells at the early stages of decidualization.

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Section: Discussionsupporting

confidence: 58%

Progesterone withdrawal and RU-486 treatment stimulate apoptosis in specific uterine decidual cells

et al. 1997

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“…Epithelial cell elimination at the time of implantation is inhibited when pregnant mice are treated with actinomycin-D, an inhibitor of mRNA synthesis. 98 As gestation advances, epithelial degeneration extends to the mesometrial side of the implantation chamber. In contrast to the degenerating epithelial cells in the antimesometrial region, the second wave of cell death is characterized by the sloughing of large sheets of adherent cells into the residual uterine lumen.…”

Section: Cell Death In the Uterine Epitheliummentioning

confidence: 99%

Apoptosis at the time of embryo implantation in mouse and rat

1999

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The aim of this review is to summarize the information currently available regarding the occurrence of apoptosis in the developing embryo and in the receptive uterus during the peri-implantation period of gestation. Cell death is detected in the inner cell mass of late pre-implantation embryos as the result of an eliminative process that helps trim the embryonic cell lineages of surplus or dysfunctional stem cells. Cell death is also detected in the epiblastic core of early postimplantation embryos, where the process is implicated in the formation of the pro-amniotic cavity. On the maternal side, uterine epithelial cells situated around the attachment site undergo cell death during the initial phase of implantation in order to facilitate embryo anchorage and access to maternal blood supply. Uterine stromal cells closest to the implantation chamber first transform into decidual cells and then commit suicide to make room for the rapidly growing embryo. Although apoptosis is well recognized as a crucial determinant of successful peri-implantation development, our understanding of the cellular and molecular mechanisms regulating this process clearly lags behind the comprehension of cell death control in other systems.

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“…Whatever the nature of the stimulus, neither it nor the mechanism for its reception is blocked by the administration of actinomycin D (Finn and Martin, 1972b;Finn and Bredl, 1973). However, since the drug was administered systemically to the mother, it probably did not reach very high concentrations in the blastocyst, and the experiments do not therefore provide evidence for or against any mechanism for the production of the decidual stimulus by the blastocyst, although they suggest that the uterine reception and transduction of the message are not dependent on transcription of DNA.…”

Section: The Nidat01y Stimulusmentioning

confidence: 99%

The Implantation Reaction

Finn

1977

Biology of the Uterus

171

121

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Implantation involves the attachment of the blastocyst to the wall of the uterus with, in some cases, the embedding of the entire ovum in the uterine wall. Associated with it are very profound and characteristic changes in the endometrium both during preparation for implantation and following attachment of the blastocyst. In this chapter, the uterine changes that take place during preparation will be considered first and then the changes during the process of implantation. The former occur regularly and frequently throughout the life of most female mammals, whereas the latter are rarer events, especially in women. This division of the subject is also convenient for the consideration of the control mechanism involved. Whereas the preparative changes are largely directed by the varying levels of the hormones secreted by the ovary, once the implantation process has been initiated hormones playa largely permissive role.There is considerable species variation in the reaction of the uterus in preparation for and during implantation. De Feo (1967) reviewed this subject very thoroughly in the first edition of this book. The great majority of research investigations have been carried out on laboratory rodents, with very little on primates or the larger domestic animals. It follows therefore that any general account of implantation will lean heavily on information acquired from laboratory animals. Although one would like to have a much more general coverage, one cannot escape the reality that information acquired from many experiments on large numbers of laboratory animals provides a sound foundation on which to build. Information from primates or wild mammals will always tend to be fragmentary because of the difficulty of obtaining large numbers of specimens. Anyone who has worked with rats or mice knows how easy it is to be misled by the results from two or three animals. The only sensible course therefore is to get as

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Studies on the Development of the Implantation Reaction in the Mouse Uterus: Influence of Actinomycin D

Cited by 41 publications

References 8 publications

Progesterone withdrawal and RU-486 treatment stimulate apoptosis in specific uterine decidual cells

Progesterone withdrawal and RU-486 treatment stimulate apoptosis in specific uterine decidual cells

Apoptosis at the time of embryo implantation in mouse and rat

The Implantation Reaction

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