Studies on the electrochemical behavior of a cystine self-assembled monolayer modified electrode using ferrocyanide as a probe

Pedrosa, Valber A.; Paixão, Thiago R.L.C.; Freire, Renato S.; Bertotti, Mauro

doi:10.1016/j.jelechem.2006.12.011

Cited by 19 publications

(11 citation statements)

References 42 publications

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“…In this case, cysteamine was selected as alkanethiol taking in consideration that glutaraldehyde will be used to link the AChE to cysteamine. Firstly, the effect of cysteamine coverage of Au-SPE on ferricyanide response was investigated, because the electron transfer for redox reactions of different molecules at SAM can be controlled by electrostatic and hydrophobic effects [41].…”

Section: Ferricyanide Behaviour At Au-spe Modified With a Sam Of Cystmentioning

confidence: 99%

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

Arduini

Guidone

Amine

et al. 2013

Sensors and Actuators B: Chemical

117

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a b s t r a c tA mono-enzymatic acetylcholinesterase (AChE) amperometric biosensor for organophosphate detection was developed immobilizing the AChE enzyme via glutaraldehyde on a preformed cysteamine selfassembled monolayer (SAM) on gold-screen printed electrodes (Au-SPEs). The enzymatic activity was monitored measuring the enzymatic product, thiocholine, at an applied potential of +400 mV vs. Ag/AgCl using ferricyanide in solution as electrochemical mediator. The electrocatalytic activity of ferricyanide towards thiocholine was investigated by using Nicholson-Shain method and finding a second order homogenous rate constant k s equal to (5.26 ± 0.65) × 10 4 M −1 s −1 . In order to develop a sensitive biosensor, the effect of cysteamine concentration, duration of SAM deposition and AChE concentration were optimized. Using paraoxon as model compound, the biosensor showed a linear range up to 40 ppb with a detection limit of 2 ppb (10% of inhibition). The biosensor was successfully challenged with drinking water sample demonstrating to be a useful analytical tool for organophosphorus insecticide detection.

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Section: Ferricyanide Behaviour At Au-spe Modified With a Sam Of Cystmentioning

confidence: 99%

Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection

Arduini

Guidone

Amine

et al. 2013

Sensors and Actuators B: Chemical

117

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“…Self-assembled monolayers (SAMs) are formed by a spontaneous physical or chemical adsorption of organic molecules forming a single layer in the surface of a substrate due to the attractive forces between specific functional groups in the organic moiety and the surface of the substrate [1]. SAMs offer certain advantages like high endurance, molecular control, and easy preparation and hence, SAMs have grown to be a powerful research tool for surface modification [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…SAMs offer certain advantages like high endurance, molecular control, and easy preparation and hence, SAMs have grown to be a powerful research tool for surface modification [1][2][3]. The most studied and well understood SAMs are alkanethiols on gold (Au) because of high binding affinity of sulfur to Au [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Self-assembled monolayers of l-cysteine on palladium electrodes

Feliciano-Ramos

Cabán-Acevedo

Scibioh

et al. 2010

Journal of Electroanalytical Chemistry

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“…Once adsorbed to the interface, the cysteine monolayer presents both amine and carboxylic acid groups to the adjacent solution. The terminal -NH 2 and -COOH groups are available for ionization by altering the solution pH, allowing electrostatic deposition of the appropriately charged polyelectrolyte [16]. The deposition of polyelectrolyte is monitored by electrochemical impedance spectroscopy and quartz crystal microbalance measurements.…”

Section: Introductionmentioning

confidence: 99%