Studies on the genetic and non‐genetic (physiological) variation of human erythrocyte glutamic oxaloacetic transaminase

Abstract

The thermostability profile of seven different electrophoretic variants of human erythrocyte GOT found in 13 different, unrelated families from a racially heterogeneous population was examined. The five different slow-variant and the two different fast-variant classes could be grouped into four different thermostability classes which were termed unstable, less stable, normal and more stable than normal. The thermostability differences among and within the electrophoretic variant classes permitted differentiation of the 13 individuals possessing an electrophoretic variant phenotype into a total of ten different variants.

Electrophoretic variants of blood proteins in Japanese VII. Cytoplasmic glutamate-oxaloacetate transaminase (GOT1)

“…and 4NG1 in this buffer system is smaller than that in the Triscitrate buffer system. On the basis of the migration rate observed in this buffer system, 2HR1 and 3NG1 or 4NGI seem to correspond to 2A and 3A, respectively, of Wurzinger and Mohrenweiser (1982) encountered in individuals of Oriental ancestry: the ratio of the distance between the homodimer band of 2HR1 and the homodimer band of 1 in the 1-2HR1 pattern to that between the homodimer band of 3NG1 or 4NG1 and the homodimer band of ! in the 1-3NG1 or I-4NG1 pattern was the same as the ratio of that in the 1-2A to that in the I-3A shown in their diagram.…”

“…In addition, normal or increased thermostability has been encountered in three new variants, 1-5NG1, 1-6HRI and 1-TNGI. Wurzinger and Mohrenweiser (1982) observed the difference in thermostability of GOT1 1 phenotype by sex and were able to subtype electrophoretic variants on the basis of thermostability profiles. Therefore, we calculated by sex the means and SDs of the percent remaining activities observed in our experiments of various phenotypes.…”

“…The same phenomenon was observed in photograph of 2-3 in the report of Ishimoto and Kuwata (1974). Since Wurzinger and Mohrenweiser (1982) succeeded in classifying two kinds of slow variants into five subtypes with a discontinuous buffer system of pH 7.5, we examined all the cases of variants with this buffer system. Results are shown…”

“…The means and the SDs for type 1 and type 1-2HR1 were obtained from 3,505 and 113 Hiroshima children, respectively, examined in screening for deficiency variants of red cell enzymes; a part of these data have already been reported (Satoh et al, 1983). Inasmuch as Wurzinger and Mohrenweiser (1982) presented data that the level of erythrocyte GOT1 1 activity of non-pregnant females (five individuals) is 1.3 times higher than that of males (thirteen individuals), we calculated the means and SDs by sex: mean SD (IU/gHb) for type 1 from 1,802 females and 1,703 males are 4.52 + 0.66 and 4.73 +_ 0.66, respectively, for type 1-2HR1 from 52 females and 61 males are 3.68 +0A9 and 3.81 +0.50, respectively. In addi- tion to these values, we observed that the differences in the means of the activities of other variant phenotypes between the females and males were less than 1SD (data not shown).…”

“…Defluorescent dark bands of GOT1 appeared against a fluorescent background of NADH2. For comparison of variants, a discontinuous buffer system of pH 7.5 (Wurzinger and Mohrenweiser, 1982) as described below was also used. The bridge buffer contained 0.44 M boric acid, 0.04 M Tris and 0.04 M LiOH.…”