Studies on the Genus Garra (Teleostei: Cyprinidae) from the Kaladan Drainage of Mizoram, India, and Synonymization of G. tyao with G. rakhinica

Abstract

Objectives: An integrative taxonomical approach to study the genus Garra from the Kaladan drainage was undertaken to evaluate the morphological characteristics as well as the phylogenetic relationship of the species. Methods: Fish samples were collected using nets and employing local fishermen. Tissues were collected preferably from the right side of the body. Whole specimens were preserved in 10% formalin or 90-95% ethanol. Genomic DNA was extracted and selected genes markers were quantified. Partial mitochondrial cytochrome c oxidase subunit I (COI) was used as the selected marker. Findings: A total of 152 specimens were collected which accounts for 8 species of the genus Garra. Based on the study, it was also concluded that Garra tyao is a junior synonym of Garra rakhinica. Novelty: This research is primarily to investigate the genus Garra from the Kaladan drainage and to provide a systematic account as well as to establish the phylogenetic lineage of the species from its congeners.