Studies on the hemolytic activity of tentacle extracts of jellyfish Rhopilema esculentum Kishinouye: Application of orthogonal test

Yu, Huan; Xing, Ronge; Liu, Song; Li, Cuiping; Guo, Zhanyong; Li, Pengcheng

doi:10.1016/j.ijbiomac.2006.06.020

Cited by 18 publications

(8 citation statements)

References 27 publications

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“…Thus, efficient utilization of the jellyfish has become a great concern. Although several active compounds have been identified from jellyfish (Yu et al, 2005b;2007a;2007b;Li et al, 2005), no studies on the extraction of glycoproteins from jellyfish have been reported.…”

Section: Introductionmentioning

confidence: 99%

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

Ren

Zhao

et al. 2009

J. Ocean Univ. China

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In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL -1 . The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.

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Section: Introductionmentioning

confidence: 99%

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

Ren

Zhao

et al. 2009

J. Ocean Univ. China

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“…The definition of parameter Y is

\max {K_{I}, K_{I I}, K_{I I I}} - \max {K_{I}, K_{I I}, K_{I I I}}

, and Y scales the effect of variables on the result. A high Y value of a certain variable means that this variable has a relatively strong effect on the result [48,49]. Obviously, the Y value of column D (error) should be the minimum among the four columns, which means the experimental results are reliable.…”

Section: Influence Of Pitting Geometry On the Tensile Behavior Of mentioning

confidence: 99%

The Corrosion Characteristics and Tensile Behavior of Reinforcement under Coupled Carbonation and Static Loading

2015

Materials

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This paper describes the non-uniform corrosion characteristics and mechanical properties of reinforcement under coupled action of carbonation and static loading. The two parameters, namely area-box (AB) value and arithmetical mean deviation (Ra), are adopted to characterize the corrosion morphology and pitting distribution from experimental observations. The results show that the static loading affects the corrosion characteristics of reinforcement. Local stress concentration in corroded reinforcement caused by tensile stress drives the corrosion pit pattern to be more irregular. The orthogonal test results from finite element simulations show that pit shape and pit depth are the two significant factors affecting the tensile behavior of reinforcement. Under the condition of similar corrosion mass loss ratio, the maximum plastic strain of corroded reinforcement increases with the increase of Ra and load time-history significantly.

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“…And the analysis of variance was performed according to refs. [20][21][22] and the results are listed in Table 4. The analyses of experiments presented in Table 4 show that F B (9.388) is above F 0.90 (2, 2) = 9.00 and F C (2.952) is below F 0.75 (2, 2) = 3.00.…”

Section: Orthogonal Testmentioning

confidence: 99%

Biological decomposition of Na2S2O3 into sulfur by a newly isolated facultative thermophilic alkaline desulphuricant strain

Zhang

et al. 2009

Sci. China Ser. B-Chem.

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A facultative thermophilic alkaline desulphuricant strain named GDJ-3 was isolated from neutral soils, enriched on sulfur synthetic medium, and detected in previous work. Conventional and chemotaxonomic analyses and 16s RNA gene sequencing showed that the strain was in good agreement with Alpha proteobacterium sp. (97%) and ochrobactrum sp. (98%). The regenerative processes of the solution containing sulfur compounds (SCS) from the strain through an orthogonal test were investigated to get the optimum regenerative condition. The results showed that regenerative temperature, air flow, and stirring speed of the agitator were the main three variables influencing the regenerative processes of the SCS. The optimum regenerative efficiency of the SCS from the strain was obtained when temperature, air flow, and stirring speed of the agitator were 318.2 K, 3.0 L/min, and zero r/min, respectively. Under this condition, when the cell concentration of the strain was adjusted to 10 7 /mL, the concentrations of Na 2 S 2 O 3 and Na 2 S in the SCS decreased from 112.68 g/L to 96.88 g/L and from 0. 87 g/L to 0.11 g/L in 9.5 h. Meanwhile, XRD spectrum shows that sulfur was formed in the regeneration process. These results suggest that the strain has potential application to the regeneration of the industrial solution containing sulfur compounds.

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Studies on the hemolytic activity of tentacle extracts of jellyfish Rhopilema esculentum Kishinouye: Application of orthogonal test

Cited by 18 publications

References 27 publications

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

The Corrosion Characteristics and Tensile Behavior of Reinforcement under Coupled Carbonation and Static Loading

Biological decomposition of Na2S2O3 into sulfur by a newly isolated facultative thermophilic alkaline desulphuricant strain

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