Studies on the intracellular localization and incorporation of 59Fe into catalase in rat liver

Radhakrishnan, T.; Sarma, Phulen

doi:10.1042/bj0930440

Cited by 10 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the isolation procedure of mitochondria yields 95% pure mitochondria with only 5% contamination of peroxisomes (21), the high catalase activity in the mitochondrial fraction indicates the presence of catalase in rat mitochondria in the current study. The presence of catalase in rat liver mitochondria (32) and rat heart mitochondria (14) were reported previously. Since catalase decomposes hydrogen peroxide specifically, the dose-dependent increase in M-catalase activity in this study initiated by increasing doses of cadmium (50 and 100 μM) suggests that hydrogen peroxide is produced in increasing amounts with increasing concentrations of cadmium.…”

Section: Discussionsupporting

confidence: 55%

Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells

Latinwo

Badisa²,

Ikediobi³

et al. 2006

Int J Mol Med

View full text Add to dashboard Cite

Cadmium affects human health through occupational and environmental exposure. In this report, we present the response of mitochondrial and cytoplasmic antioxidant enzymes of CRL-1439 cells exposed to different concentrations (0-150 μM) of CdCl 2 for 24 h at 37˚C. Exposure of liver cells to 50 μM CdCl 2 increased mitochondrial catalase and glutathione reductase (GR) activities more than the cytoplasmic enzymes. Although the mitochondrial selenium-dependent glutathione peroxidase (Se-GPx) showed less enzymatic activity than the cytoplasmic enzyme, the mitochondrial selenium-independent glutathione peroxidase (non-Se-GPx) showed a slight increase in activity over its cytoplasmic counterpart compared to untreated controls. With 100 μM CdCl 2 , catalase maintained an increase in specific activity in mitochondria over the cytoplasmic enzyme compared to the controls. The level of GR was higher in the cytoplasm than in the mitochondria. However, the activity of Se-GPx and non-Se-GPx decreased slightly in the mitochondria compared to their cytoplasmic counterparts. Exposure of cells to 150 μM CdCl 2 decreased all antioxidant enzyme activities compared to the 100 μM CdCl 2-treated samples due to toxic effect. Each antioxidant enzyme exhibited its own pattern of activation or inhibition upon exposure to different concentrations of cadmium, with more oxidative stress observed in the mitochondria.

show abstract

Section: Discussionsupporting

confidence: 55%

Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells

Latinwo

Badisa²,

Ikediobi³

et al. 2006

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…The studies of the rate of incorporation of Fe59 [4,26,34,41,47], C14-leucine [21,22], C14-glycine [35] into the catalase molecule accords with this view.…”

Section: Introductionsupporting

confidence: 59%

The Roles of Synthesis and Degradation in the Regulation of Catalase Levels in the Neoplastic Tissues

Rechcigl¹,

Hruban²,

Hp³

1969

Enzymol Biol Clin

View full text Add to dashboard Cite

Summary. Several rat hepatomas were analyzed as to their catalase activity. Ample enzyme activity was found in each of the following tumors : the HC-hepatoma and hepatomas No. 7316, 7794, 7800, H-139, and H-122. Moderate activity was also seen in the LC-hepatoma, in various lines of 5123 hepatoma, and in hepatomas No. H-35, 7288C, 7787, 7793, 7795, 9098, and 9108. The rapidly growing hepatomas, such as the Novikoff, and hepatomas No. 3683, and 3924A were virtually devoid of any activity. Attempts have been made at the measurement of the rate of catalase synthesis and degradation in neoplastic tissues, as well as in the tissues of the tumor-bearing hosts. Advantage was taken of the methods elaborated in our laboratory, based on the novel properties of certain inhibitors to irreversibly inactivate catalase, or to block its synthesis. Using 5123 hepatoma, it was determined that during each hour 3.88 units of catalase were being synthesized per gram of the host liver, as compared to 0.92 units in the tumor. During the same one-hour period 2.4% of the catalase molecules present were being destroyed in both types of tissues. In the case of 7316A hepatoma, there were only 2.30 units catalase formed in an hour which can be contrasted with 4.00 units in the host liver. The rates of destruction in the respective tissues were 2.6 and 1.9% per h. The kinetic studies endeavored in some of the other tumors, particularly the HC-hepatoma, were largely unsuccessful because of their failure to respond to the inhibitors. The lack of response to 3-amino-l,2,4-triazole may be related to absence or low activity of hydrogen peroxide producing oxidases within microbodies, absence of microbodies, or to low permeability of the microbody (or the cell) to the drug. Hepatoma 7794A does not contain typical microbodies.

show abstract

“…The catalase enzyme activity has been measured according to published report( 32 . Initially, a reaction mixer was prepared by mixing 500 µl of 0.1 (M) phosphate buffer (pH = 7.5) and 500 µl of freshly prepared 0.9 % H 2 O 2 solution (say RM solution).…”

Section: Methodsmentioning

confidence: 99%

Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System)

et al. 2014

View full text Add to dashboard Cite

In this report, we have designed a simple and efficient green chemistry approach for the synthesis of colloidal silver nanoparticles (b-AgNPs) that is formed by the reduction of silver nitrate (AgNO3) solution using Olax scandens leaf extract. The colloidal b-AgNPs, characterized by various physico-chemical techniques exhibit multifunctional biological activities (4-in-1 system). Firstly, bio-synthesized silver nanoparticles (b-AgNPs) shows enhanced antibacterial activity compared to chemically synthesize silver nanoparticles (c-AgNPs). Secondly, b-AgNPs show anti-cancer activities to different cancer cells (A549: human lung cancer cell lines, B16: mouse melanoma cell line & MCF7: human breast cancer cells) (anti-cancer). Thirdly, these nanoparticles are biocompatible to rat cardiomyoblast normal cell line (H9C2), human umbilical vein endothelial cells (HUVEC) and Chinese hamster ovary cells (CHO) which indicates the future application of b-AgNPs as drug delivery vehicle. Finally, the bio-synthesized AgNPs show bright red fluorescence inside the cells that could be utilized to detect the localization of drug molecules inside the cancer cells (a diagnostic approach). All results together demonstrate the multifunctional biological activities of bio-synthesized AgNPs (4-in-1 system) that could be applied as (i) anti-bacterial & (ii) anti-cancer agent, (iii) drug delivery vehicle, and (iv) imaging facilitator. To the best of our knowledge, there is not a single report of biosynthesized AgNPs that demonstrates the versatile applications (4-in-1 system) towards various biomedical applications. Additionally, a plausible mechanistic approach has been explored for the synthesis of b-AgNPs and its anti-bacterial as well as anti-cancer activity. We strongly believe that bio-synthesized AgNPs will open a new direction towards various biomedical applications in near future.

show abstract

Studies on the intracellular localization and incorporation of 59Fe into catalase in rat liver

Abstract: was homogenized in a medium containing sodium-potassium phosphate buffer, pH 7-4 (0-154M), protoporphyrin (900 ,m), NaHCO3 (38-5 mm), GSH (1 mM) and P-mercapto-Vol. 93

Cited by 10 publications

References 30 publications

Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells

Effect of cadmium-induced oxidative stress on antioxidative enzymes in mitochondria and cytoplasm of CRL-1439 rat liver cells

The Roles of Synthesis and Degradation in the Regulation of Catalase Levels in the Neoplastic Tissues

Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System)

Contact Info

Product

Resources

About