Studies on the Procurement of Blood Coagulation Factor VIII

Abstract

To determine the viability of blood cells collected in half-strength (0.5 CPD.A2) and full-strength citrate anticoagulants, paired crossover autologous survival studies were performed in normal volunteers using 51Cr-labelled red cells after 35 days storage and 111In-labelled platelets after 5 days storage. For both studies, viability was better maintained in 0.5 CPD.A2 anticoagulant. This was significant for red cells (24-hour survival: 80 ± 6 vs. 52 ±21%) but not for platelets by either linear or multiple-hit models (recovery 51 ± 13 vs. 38 ± 14%, survival 5.1 ± 1.4 vs. 3.7 ± 1.5 days). The poor viability of red cells after storage in full-strength anticoagulant was associated with low cellular adenosine triphosphate levels and was confirmed in follow-up studies.