Studies on the relationship between adeno-associated virus type 1 (AAV-1) and adenoviruses

Casto, Bruce C.; Armstrong, John A.; Atchison, Robert W.; Hammon, William McD.

doi:10.1016/0042-6822(67)90120-1

Cited by 55 publications

(33 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to autoregulation, the AAV genome has previously been demonstrated to inhibit Ad plaque formation, DNA replication, and the expression of the major product of the Ad early region 1B, the 55-kilodalton tumor antigen (6,7,32). In this paper we showed that the AAV genome can inhibit transformation by genes under the control of other viral promoters (SV40 early promoter and herpes simplex virus tk promoter) and of a murine promoter (the inducible metallothionein promoter).…”

Section: Discussionmentioning

confidence: 79%

See 1 more Smart Citation

Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes.

Labow¹,

Graf²,

Berns³

1987

Mol. Cell. Biol.

128

View full text Add to dashboard Cite

In this paper we report that adeno-associated virus (AAV) genomes inhibit stable transformation by several dominant selectable marker genes upon cotransfection into mouse tissue culture cells. Cotransfection of AAV genomes also inhibited the expression of pSV2cat in transient assays. In both cases, the inhibitory effect was independent of AAV DNA replication but required the AAV p5 and p19 genes, which encode proteins required for AAV DNA replication and regulation of AAV gene expression. Finally, addition of a cloned E4 gene in the transfection experiments partially blocked the AAV-mediated inhibitory activities.

show abstract

Section: Discussionmentioning

confidence: 79%

“…AAV coinfection inhibits Ad DNA replication and virus production (6,7). AAV coinfection also inhibits the ability of Ad both to transform cells and to cause tumors when injected into susceptible animals (24,30).…”

mentioning

confidence: 99%

Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes.

Labow¹,

Graf²,

Berns³

1987

Mol. Cell. Biol.

128

View full text Add to dashboard Cite

show abstract

“…Wild-type (wt) AAV-2 particles have been observed in cervical 15 as well as in respiratory epithelium. 16 Wt AAV-2 is replication-defective, requiring helper virus functions from adenovirus or herpes virus to replicate efficiently, 17,18 and has not been associated with any disease as of yet. As demonstrated by Rosenfeld et al 19 and KleinBauernschmitt et al, 20 leptomeningeal spread of solid tumors or solid tumor cell lines seems to be susceptible to AAV-2-mediated gene transfer.…”

mentioning

confidence: 99%

Differential expression of a recombinant adeno-associated virus 2 vector in human CD34+ cells and breast cancer cells

et al. 2000

View full text Add to dashboard Cite

The use of autologous hematopoietic stem cell (HSC) grafts after high-dose chemotherapy protocols may be hampered by contamination of the grafts with tumor cells. Because epithelial cells seem to be the natural hosts of adeno-associated virus 2 (AAV-2), we speculated that epithelial tumor cells in HSC grafts might be selective targets for AAV-2-based vectors. To test this hypothesis, the breast cancer cell lines T47D and MCF-7 were infected with a recombinant AAV-2 vector expressing the green fluorescent protein (GFP) gene; in addition, human CD34 ϩ mobilized peripheral progenitor cells were infected with the same vector. At a multiplicity of infection of 100, only 1.39% Ϯ 0.51% CD34 ϩ cells expressed the GFP gene whereas, 36.06% Ϯ 6.53% of the infected T47D cells and 41.52% Ϯ 3.16% of the infected MCF-7 cells expressed the transduced GFP gene. After further optimizing the transduction procedure by using higher multiplicities of infection (100 -500) and preincubation of samples with the tyrosine kinase inhibitor genistein, up to 82.52% and 85.35% GFP ϩ T47D and MCF-7 cells, respectively, were observed. The GFP fluorescence intensity in transduced mammary tumor cells was up to 3 logs higher than that of transduced CD34 ϩ cells. The differential expression of recombinant AAV-2 vectors in hematopoietic and epithelial tumor cells warrants further research with this vector system, including the use of suicide genes for the purging of autologous HSC grafts. Cancer Gene Therapy (2000) 7, 597-604

show abstract

“…This was proposed to be due to an inhibition of adenovirus replication by the AAV reps, although the mechanism underlying the inhibition has not been established. 13,14 Four Rep proteins are encoded within a single open reading frame of AAV. These four Rep proteins include Rep78, Rep68, Rep52, and Rep40, which are produced by alternative RNA splicing of RNA products that are transcribed from two different translation codons.…”

Section: Introductionmentioning

confidence: 99%

“…2,6 Rep78 and Rep68, which are produced from the same p5 promoter have been proposed to be toxic. 13,14 These two proteins, which are essential not only for site-specific integration but also for AAV replication, have several biochemical activities in common and both interact with the DNA-specific binding site of Rep. 15 Both Rep78 and Rep68 exhibit strand-and site-specific endonuclease activity, 16 as well as an ATP-dependent DNA-DNA and DNA-RNA helicase activity. 17 Both are able to modulate the activity of endogenous as well as heterologous promoters.…”

Section: Introductionmentioning

confidence: 99%

Recombinant adenovirus expressing adeno-associated virus cap and rep proteins supports production of high-titer recombinant adeno-associated virus

et al. 2001

View full text Add to dashboard Cite

It has been difficult to produce a chimeric vector containing both Ad and AAV rep and cap, and to grow such chimeric vectors in 293 cells. By recombination in vitro in a bacterial host, we were able to produce recombinant plasmid AdAAV (pAdAAVrep-cap), which could be used to generate recombinant AdAAV (rAdAAVrep-cap) after transfection into 293 cells. A recombinant adenovirus, rAdAAVGFP, in which the green fluorescent protein (GFP) gene is flanked by the AAV terminal repeats cloned into the E1-deleted site of Ad was also generated. Co-infection of rAdAAVrep-cap together with rAdAAVGFP into 293 cells resulted in production of high tit-

show abstract

Studies on the relationship between adeno-associated virus type 1 (AAV-1) and adenoviruses

Cited by 55 publications

References 21 publications

Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes.

Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes.

Differential expression of a recombinant adeno-associated virus 2 vector in human CD34+ cells and breast cancer cells

Recombinant adenovirus expressing adeno-associated virus cap and rep proteins supports production of high-titer recombinant adeno-associated virus

Contact Info

Product

Resources

About