Studies on the surgical induction of endometriosis in the rat

Vernon, Michael; Wilson, Emery A.

doi:10.1016/s0015-0282(16)48988-0

Cited by 409 publications

(58 citation statements)

References 13 publications

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“…Therefore, rat is a relatively ideal animal model for establishing model of endometriosis. Early in 1985, researchers had established a classical endometriosis model via surgical transplantation to evaluate the efficacy of antiendometriosis candidates [27, 28]. We had applied this model to confirm the effectiveness of biodegradable microspheres containing nomegestrol acetate on suppressing the growth of endometriotic tissues in previous study [21].…”

Section: Discussionmentioning

confidence: 99%

Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

Zhong

Zhu

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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We evaluated the effectiveness of Kuntai Capsule (KTC) for treating endometriosis using rat model and investigated its preliminary mechanism of action involved. SD rats were implanted with endometrial tissues and treated with KTC for three weeks. Then, laparotomy was performed to examine volume changes of the autografts. The serum levels of TNF-α, IL-6, COX-2, E2, and P4 were measured through ELISA. TUNEL was performed to analyze the apoptosis on ectopic endometrium. Protein levels of caspases 8, 9, and 3 and cytochrome c in the ectopic and eutopic endometrium were measured by western blotting. Results showed that KTC significantly decreased the volumes of ectopic endometrium. The level of TNF-α increased and E2 decreased in the KTC treatment groups. TUNEL and western blot assay showed that KTC could induce apoptosis of endometriotic tissues, accompanied with the increased protein expression of caspases 8 and 9, activated caspase-3, and cytochrome c in a dose-dependent manner. However, these protein expression profiles were not affected in eutopic endometrium. Our findings suggest that KTC could inhibit the growth of ectopic endometrial tissue through upregulating the level of TNF-α and its downstream signaling, including caspases and cytochrome c.

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Section: Discussionmentioning

confidence: 99%

Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

Zhong

Zhu

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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“…It has been suggested that an autoimmune response against endometrial implants might be responsible for the presence of these cells. However, Vernon and Wilson [19] postulated that the autoimmune response was raised against the uterine secretions rather than against the endometrial tissue, due to the lack of evidence of cell death.…”

Section: Discussionmentioning

confidence: 99%

Cellular Exchange in an Endometriosis-Adhesion Model Using GFP Transgenic Mice

Wilkosz¹,

Pullen

de-Giorgio-Miller

et al. 2011

Gynecol Obstet Invest

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Background: Endometriosis is a debilitating disease that affects women of reproductive age and may lead to impaired fertility. Cell attachment, invasion of the underlying tissue, and vascular ingrowth are important processes in endometrial lesion development. However, the degree of cellular exchange between host peritoneum and endometrial tissue is unclear. Methods: An experimental endometriosis model was employed whereby uterine horn fragments from wild-type mice were implanted into genetically identical eGFP (enhanced green fluorescent protein) host mice and vice versa. Hormone sensitivity of the ectopic lesions was assessed and cellular exchange determined histologically. Results: White cyst-like lesions developed from implanted fibrin-rich fragments by day 7. Lesions consisted of a well-developed stroma with glandular and luminal epithelium. Both ovariectomy and treatment with a GnRH agonist, leuprorelin, resulted in the suppression of ectopic lesion growth, whereas estradiol treatment increased the size of the ectopic lesion (4 mice per group on day 14). Ingrowth and outgrowth of blood vessels was apparent as well as the exchange of cells between host peritoneum and lesion. Conclusion: These findings support the proposal that there is a close cellular interplay between host peritoneum and ectopic tissue and the suitability of this mouse model to study these interactions.

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“…Первые аутологичные трансплантации матки были проведены у кроликов [27], крыс [28] и хомя-ков [29]. Процедура включала удаление одного из маточных рогов при минилапаротомии.…”

Section: модели эндометриоза на грызунахunclassified

Experimental endometriosis models: different approaches for different tasks

Petrosyan¹

2016

Probl. reprod.

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В настоящее время эндометриоз признается од-ним из самых загадочных заболеваний, затрагиваю-щих миллионы женщин по всему миру. Несмотря на более чем полувековой опыт исследования, этиоло-гия и патогенез этого заболевания так и остаются до конца не ясными. Ввиду разнообразия клинических проявлений эндометриоз является труднодиагно-стируемым заболеванием, особенно на ранних ста-диях. Неинвазивные методы диагностики малоспе-цифичны, «золотым стандартом» является лапаро-скопическое исследование, ценностью которого яв-ляется одновременно с диагностикой хирургическое лечение эндометриоза. Однако частота рецидивов не позволяет считать этот метод лечения высокоэффек-тивным. Современный традиционный подход к те-рапии больных эндометриозом предусматривает комбинацию хирургического метода (максимальное удаление патологических очагов в пределах неизме-e-mail: mariya@labpharm.spb.ru ненных тканей) и супрессивной гормональной тера-пии, целью которой является временное угнетение функции яичников, направленное на уменьшение секреции эстрадиола, приводящее к регрессу эндо-метриоидных гетеротопий, хотя активно разрабаты-ваются и новые подходы [1][2][3][4].На сегодняшний день лечение больных эндоме-триозом представляет одну из важнейших проблем современной гинекологии. Не существует, а возмож-но, и не может существовать, единого подхода и уни-версального метода лечения, который гарантировал бы облегчение симптомов, приводил бы к полному излечению и отсутствию рецидивов. В связи с этим поиск новых стратегий терапии эндометриоза явля-ется крайне актуальной задачей, особенно в контек-сте развития персонифицированной медицины.Одной из основных причин отсутствия прогресса в лечении эндометриоза является трудность изучения обзор посвящен описанию различных моделей эндометриоза. Даны представления о существующих подходах к моделированию данного заболевания у разных видов животных: приматов, грызунов, трансгенных животных, а также на хорион-аллантоисной оболочке куриного эмбриона. Показаны основные направления применения экспериментальных моделей, а именно: изучение патофизиологических механизмов заболевания, разработка новых неинвазивных методов диагностики, поиск новых подходов к терапии эндометриоза. Проведен разбор преимуществ и ограничений при использовании каждой модели. -Petersburg, russia, 199034 this review discusses the experimental models of endometriosis. the general idea about available approaches to the modeling of this disease in different animal species, namely primates, rodents, transgenic animals, as well as on the chorioallantoic membrane of the chicken embryo is presented. Main implementations of the experimental models are suggested. Among those are pathophysiological mechanisms of the disease, the development of new non-invasive diagnostic methods, and a search for new approaches to the treatment of endometriosis. the benefits and limitations of each model implementation are analized.Keywords: endometriosis, animal models of endometriosis, pathophysiological mechanisms of endometriosis, diagnosis and therapy of e...

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Studies on the surgical induction of endometriosis in the rat

Cited by 409 publications

References 13 publications

Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

Kuntai Capsule Inhibited Endometriosis via Inducing Apoptosis in a Rat Model

Cellular Exchange in an Endometriosis-Adhesion Model Using GFP Transgenic Mice

Experimental endometriosis models: different approaches for different tasks

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