Studies on toxicity of multi-walled carbon nanotubes on suspension rice cells

Tan, Xiao; Lin, Chun Yi; Fugetsu, Bunshi

doi:10.1016/j.carbon.2009.08.018

Cited by 250 publications

(119 citation statements)

References 31 publications

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“…In contrast with the investigations of other nanoparticles described in the literature [3,15,25,26], in this study, nearly all of the plant cells retained high viability after the longest incubation time (24 h) with the highest concentration (1 mg mL 1 ) of MSNs or A-MSNs.…”

Section: Cytotoxicity Of Msns For Liriodendron Hybrid Suspension Cellscontrasting

confidence: 97%

“…Significantly, after 24 h of incubation with large added amounts of MSNs (1 mg mL 1 ), the plant cells retained high viability, and successfully developed into somatic embryos and emblings via somatic embryogenesis. In contrast with other nanomaterials such as CNTs or CdSe/ZnS QDs that show high cytotoxicity toward plant cells [15,25,26], these ultrafine MSNs with excellent biocompatibility show great potential in the field of plant biotechnology as nano-carriers for the targeted delivery of small molecules, peptides, and nucleic acids to specific organelles or host genomes at the cellular level.…”

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confidence: 99%

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Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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The characteristics of the interactions co-cultures of ultrafine mesoporous silica nanoparticles (MSNs) and the Liriodendron hybrid suspension cells were systematically investigated using laser scanning confocal microscope (LSCM) and scanning electron microscopy (SEM). Using fluorescein isothiocyanate (FITC) labeling, the LSCM observations demonstrated that MSNs (size, 5-15 nm) with attached FITC molecules efficiently penetrated walled plant cells through endocytic pathways, but free FITC could not enter the intact plant cells. The SEM measurements indicated that MSNs readily aggregated on the surface of intact plant cells, and also directly confirmed that MSNs could enter intact plant cells; this was achieved by determining the amount of silicon present. After 24 h of incubation with 1.0 mg mL 1 of MSNs, the viability of the plant cells was analyzed using fluorescein diacetate staining; the results showed that these cells retained high viability, and no cell death was observed. Interestingly, after the incubation with MSNs, the Liriodendron hybrid suspension cells retained the capability for plant regeneration via somatic embryogenesis. Our results indicate that ultrafine MSNs hold considerable potential as nano-carriers of extracellular molecules, and can be used to investigate in vitro gene-delivery in plant cells. Along with developments in the application of nanotechnology from animal science and medical research to plant science research, the impact of engineered nanomaterials on plant systems has attracted increasing attention, the areas including (i) the delivery of fertilizers, herbicides, pesticides and exogenous genes, (ii) the improvement of the growth of plants, and (iii) nanotoxicity research for plant cells [1][2][3][4][5][6][7][8][9][10]. However, compared with mammalian cells, the plant cell wall which is composed of cross-linked polysaccharides (cellulose, hemicelluloses, and pectin) represents an extra barrier surrounding the cell membrane that hinders the passage of nanoparticles into plant cells. To avoid the inhibiting effects of the plant cell wall, free protoplasts (which are prepared via the removal of the cell wall using cellulase treatments) have been used previously to study the internalization of various nanoparticles (e.g., CdSe/ZnS quantum dots (QDs), polystyrene nanospheres, poly(phenylene ethynelene) nanoparticles, and carbon nanomaterials) [11][12][13]. However, protoplast-based transformation methods hold disadvantages in that the viability of the protoplasts and their ability to divide are strongly reduced by the chemicals that are applied to disorganize the cell wall. For this reason, recent studies have focused on intact plant cells; it was found to that they are able to achieve endocytosis to directly internalize single-walled carbon nanotubes (CNTs), CdSe/ZnS QDs, or poly (amidoamine) dendrimer from the

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Section: Cytotoxicity Of Msns For Liriodendron Hybrid Suspension Cellscontrasting

confidence: 97%

mentioning

confidence: 99%

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Xia

Dong

Zhang

et al. 2012

Sci. China Life Sci.

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“…Over last few years, there have been extensive studies to understand the influence of nanomaterials on the growth of different types of plants (Khodakovskaya et al 2009;Khodakovskaya et al 2013a, b;Lu et al 2002;Cañas et al 2008;Yang et al 2006;Hong et al 2005a, b;Tan et al 2009;Yuan et al 2011;Khodakovskaya et al 2013a, b;Mahajan et al 2011;Wang et al 2012;Larue et al 2012;Miralles et al 2012;Feizi et al 2012;Tripathi et al 2011;Sonkar et al 2012). Khodakovskaya et al (2009Khodakovskaya et al ( , 2013a demonstrated that at very low doses MWCNT can penetrate the seed coat and enhance the germination of tomato plant.…”

Section: Introductionmentioning

confidence: 99%

“…Yang et al (2006) and Hong et al (2005a, b) studied the growth of the spinach and found that proper concentration of TiO 2 could enhance photosynthesis and nitrogen metabolism. Tan et al (2009) demonstrated the use of MWCNT to increase reactive oxygen species (ROS) and decrease the cell viability of rice plant. Yuan et al (2011) found that SWNT (50 lg/ml) stimulate the growth of mesophyll cells of Arabidopsis.…”

Section: Introductionmentioning

confidence: 99%

Influence of water soluble carbon dots on the growth of wheat plant

2014

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The effect of water soluble carbon nano-dots (wsCND) on the growth of root and shoot of wheat plants under light and dark conditions has been studied. The wsCND enhances the growth of root and shoot both in light and dark conditions. The effect of wsCND on the growth of root was relatively more compared to that of shoot. Scanning electron and Fluorescence microscopic analysis show that wsCNDs enter inside the plant. Raman spectroscopy also confirms this. The present study shows that wsCNDs are non-toxic to the wheat plant and can be used to enhance the production of this cereal crop.

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“…[4][5][6] However, plant uptake of ENMs is a very recent field of study and contradictory results have been reported, with some studies reporting plant accumulation of ENMs 7,8 and others showing no uptake. [9][10][11][12][13] Therefore, an attempt must be made to elucidate the pathways and mechanisms of NP uptake by plants to explain the contradictory observations regarding plant uptake. One of the most important distinguishing features of plant cells is that they are enclosed by rigid cell walls composed of cellulose, hemicelluloses and pectin with pores whose diameter is typically in the range of 3-8 nm, 14 and these allow only small molecules to pass through.…”

Section: Introductionmentioning

confidence: 99%

Accumulation, speciation and uptake pathway of ZnO nanoparticles in maize

Zhang

Luo

et al. 2015

Environ. Sci.: Nano

185

104

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Engineered nanomaterials such as ZnO nanoparticles (NPs) will inevitably enter the environment because of the large quantities produced and their widespread application. Plants comprise a fundamental living component of terrestrial ecosystems; thus, understanding the interaction between ENMs and plants is important. In the present study we conducted an integrated study by employing a combination of microscopic and spectroscopic techniques to comparatively investigate the uptake of ZnO NPs and Zn 2+ ions by maize in order to further elucidate plant uptake pathways of ZnO NPs. The results demonstrate that the majority of Zn taken up was derived from Zn 2+ released from ZnO NPs, and Zn accumulated in the form of Zn phosphate. ZnO NPs were observed mainly in the epidermis, a small fraction of ZnO NPs were present in the cortex and root tip cells, and some further entered the vascular system through the sites of the primary root-lateral root junction. However, no ZnO nanoparticle was observed to translocate to shoots, possibly due to the dissolution and transformation of ZnO NPs inside the plants.

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Studies on toxicity of multi-walled carbon nanotubes on suspension rice cells

Cited by 250 publications

References 31 publications

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells

Influence of water soluble carbon dots on the growth of wheat plant

Accumulation, speciation and uptake pathway of ZnO nanoparticles in maize

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