Studies on wheat bran Arabinoxylan for its immunostimulatory and protective effects against avian coccidiosis

Akhtar, Masood; Tariq, Ahmad Fraz; Awais, Mian Muhammad; Iqbal, Zafar; Muhammad, Faqir; Shahid, Muhammad; Hiszczyńska-Sawicka, Elżbieta

doi:10.1016/j.carbpol.2012.05.048

Cited by 73 publications

(62 citation statements)

References 44 publications

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“…The highest average of MCHC level was found in the experimental rabbits administered with aquaprim while the lowest level was found in the experimental rabbits without administration of garlic extract (0 mg). Akhtar et al (2012) stated that infection of coccidia might influence the whole hematological parameters. The hematological changes on the coccidia-infected rabbits were proven with the significantly decreasing number of erythrocytes, hemoglobin, and hematocrit as well as the increasing number of leukocytes, especially eosinophils, and neutrophils (Al-Saeed et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

Indrasanti

Indradji

Hastuti

et al. 2017

Med Pet

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This research aimed to examine the potential of garlic as the coccidiosis control in rabbits either in vitro or in vivo. During in vitro, observed variables were rabbits oocysts that were sporulated, unsporulated, and abnormal in incubation for 3 days with the addition of garlic extract. The treatments were doses of garlic extract administration (0%, 1%, 2%, 4%, and 8%) and sulfaquinoxalline as a standard anticoccidiosis. Meanwhile during in vivo, the variables observed were the hematological profile of the experimental rabbits naturally infected with coccidia. The doses of garlic extract was administered orally to the experimental rabbits infected with coccidia were 0 mg, 10 mg, 20 mg, 40 mg, and 80 mg/ rabbit. As a standard coccidiosis drugs, the combination of sulfadiazine and trimethoprim was used. The treatments were given for 6 days. The variables observed were the hematological profile of the coccidiosis rabbits, including the erythrocytes, hemoglobin, hematocrits, MCV, MCH, MCHC, and thrombocytes. The research employed a completely randomized design, with 5 repetitions. The data were further analyzed using the honestly significant difference test. The results showed that garlic extract administration significantly decreased (P<0.01) both the number of the sporulated and unsporulated oocysts (P<0.05), yet did not significantly influence the abnormal oocysts, but there was no significant difference within the entire hematological variables except in thrombocytes (P<0.05). Garlic extract administration decreased the excretion number of oocysts in the feces either in vitro or in vivo and influenced some hematological variables which provided a new propect for controlling coccidiosis naturally in rabbits.Keywords: coccidia, rabbits, garlic extract, oocyst, hematology ABSTRAK Penelitian ini bertujuan untuk menguji potensi bawang putih sebagai anti koksidiosis pada kelinci secara in vitro dan in vivo. Secara in vitro, peubah yang diamati adalah ookista kelinci yang bersporulasi, tidak bersporulasi, dan abnormal pada inkubasi selama 3 hari dengan penambahan ekstrak bawang putih. Perlakuan yang digunakan adalah 0%, 1%, 2%, 4%, dan 8% bawang putih, serta penggunaan sulfaquinoxalline sebagai obat standar koksidiosis. Pada penelitian in vivo, peubah yang diamati adalah profil hematologi kelinci yang terinfeksi koksidia secara alami. Ekstrak bawang putih diberikan secara per oral pada kelinci yang terinfeksi koksidia sebesar 0 mg, 10 mg, 20 mg, 40 mg, dan 80 mg/ekor, serta kombinasi sulfadiazin dan trimethoprim sebagai obat standar koksidiosis. Perlakuan diberikan selama 6 hari. Peubah yang diamati adalah profil darah kelinci koksidiosis yang meliputi sel darah merah (eritrosit), hemoglobin (Hb), hematokrit, MCV, MCH, MCHC, dan trombosit. Rancangan yang digunakan adalah rancangan acak lengkap (RAL), dengan pengulangan sebanyak 5 kali. Analisis data menggunakan analisis variansi dengan uji lanjut beda nyata jujur (BNJ). Hasil analisis varians menunjukkan bahwa pemberian ekstrak bawang putih berpengaruh sangat ...

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Section: Discussionmentioning

confidence: 99%

The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

Indrasanti

Indradji

Hastuti

et al. 2017

Med Pet

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“…Wheat was processed to obtain bran following the method of Akhtar et al (2012). Briefly, the bran soaked for 1 h in de-ionized water at 4°C was passed through 200 mm mesh.…”

Section: Procurement and Pre-treatment Of Bransmentioning

confidence: 99%

“…Increase in body weight from day 3 rd to 11 th and oocysts per gram (OPG) of droppings from day 4 th to 12 th post challenge were calculated (Ryley et al, 1976;Akhtar et al, 2012). Further, organ to body weight ratios and the percent protection against GIT lesions were evaluated with the formula of Singh and Gill (1975).…”

Section: Therapeutic Evaluationmentioning

confidence: 99%

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Rizwan¹,

Khan²,

Iqbal³

et al. 2016

IJAB

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To cite this paper: Rizwan, H.M., M.K. Khan, Z. Iqbal and F. Deeba, 2016. Immunological and therapeutic evaluation of wheat (Triticum aestivum) derived beta-glucans against coccidiosis in chicken. AbstractThis study was carried out to determine the immunomodulatory and therapeutic effects of wheat derived beta-glucans (purified) against avian coccidiosis. Briefly, beta-glucans from wheat bran were extracted and purified using standard procedures. A total of 250 broiler chicks (day-old) were reared at experimental station, Department of Parasitology, University of Agriculture, Faisalabad for this study. At 7 th day of their age, birds were subdivided into five equal groups (n=50). Groups A, B and C were orally administered with graded doses of purified beta-glucans (50, 100 and 150 mg/kg of body weight) for three consecutive days; whereas, positive control (group D) was administered with vitamin-E at the dose rate of 87mg/kg; while negative control (group E) was served with phosphate buffered saline (PBS solution). At 14 th day post administration of graded doses, 30 chickens from each group were randomly selected and used to monitor cellular and humoral immune responses, while remaining birds (n=20) in each group were challenged with mixed species of genus Eimeria for therapeutic evaluation. Overall, chickens in group A administered with 50 mg/kg b.wt. purified beta-glucans showed significantly higher immune responses in terms of enhanced humoral and cell mediated immunity as compared to those of other beta-glucans administered and E groups. While, the immune responses showed by group A were comparable with group D. After challenge with Eimeria, the groups A and D also showed maximum weight gains with low oocyst counts and maximum percent protection against lesions in both caecum and intestine. On the other hand, a minimum daily weight gain with increased number of oocysts in chickens was observed in control group (PBS) during therapeutic evaluation. These findings suggest that the beta-glucans derived from wheat may have immunomodulatory and therapeutic effects against Eimeria infection in chickens.

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“…Humic substances subjected to the experiment were obtained from the biotransformation of cachaza, a product of sugarcane and alcohol. This underwent a biotechnological process to transform and stabilize organic matter (80% of humic acids) to obtain a pH of 6.4, density of 0.73g/cm 3 , maximum humidity of 12.32%, and 62.45% ash. This product was supplied by PBA Biotechnology Products.…”

Section: Colecta De Muestrasmentioning

confidence: 99%

“…En aves, existen diversos trabajos que reportan el uso de inmunoestimulantes e incluyen levamisol, probióticos, prebióticos, extracto de bursa de fabricio y timo, productos vacunales (3)(4)(5) Las sustancias húmicas (SH) son el producto final de la biotransformación de la materia orgánica, entre las cuales las más comunes son los ácidos húmicos, fúlvicos y húmicomelánicos (6). Aunque estas sustancias no se reportan en las tablas de aditivos para el diseño de dietas animales, algunos estudios demuestran que éstas tienen un potencial importante como promotores de crecimiento en pollos de engorde y en gallinas ponedoras (7).…”

Section: Introductionunclassified

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

2016

Rev MVZ Córdoba

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El suplemento de sustancias húmicas afecta la inmunidad innata en gallinas ponedoras en fase posmuda ABSTRACTObjective. Asses the effect of supplementation with Humic substances (HS) over some innate immunity parameters (serum bactericidal activity, phagocytosis, bacterial agglutination, respiratory burst and lisozyme activity) in phase after fasting of layer hens. Materials and methods. 120 posfasting phase Hy Line Brown layer hens were taken which were distributed into four groups: The first and the second were supplemented with 0.1 and 0.2% of HS, respectively. The third group was supplemented with 0.25 mg/kg on levamisole hydrochloride and fourth group have no supplementation; during sixty days period. Blood samples were collected on 8th, 30th and 60th of experiment day. Results. The phagocytic index and respiratory burst increased significantly at day 30th in HS supplemented groups. Alike, serum bactericidal activity and lisozyme activity improved on 8 th day, nevertheless, changes were no evident latter. The bacterial agglutination was high in supplemented groups evaluated at everyone times. Conclusions. Results showed that HS behave as immunostimulant in the early phase after fasting layer hens.Key words: Agglutination, bactericide activity, Lisozyme, phagocytosis (Source: DeCS). RESUMENObjetivo. Evaluar el efecto de las sustancias húmicas (SH) sobre algunos parámetros de la inmunidad innata (actividad bactericida del suero, fagocitosis, aglutinación bacteriana, explosión respiratoria y actividad de la lisozima) en la fase posmuda de gallinas ponedoras. Materiales y métodos. Se utilizaron 120 gallinas ponedoras Hy Line Brown en la fase de posmuda, las cuales fueron divididas en cuatro grupos: Los dos primeros fueron suplementados con 0.1 y 0.2% de SH respectivamente, el tercer grupo fue suplementado con 0.25 mg/kg de Clorhidrato de levamisol y el cuarto grupo control sin suplemento; durante un período de 60 días. Las muestras sanguíneas se tomaron los días 8, 30 y 60 del experimento. Resultados. El Índice fagocítico y la explosión respiratoria se incrementaron significativamente a partir del día 30 de suplementación con SH. De la misma manera, la actividad bactericida del suero y la actividad de la lisozima aumentaron al día 8; no obstante no se evidenciaron cambios posteriores. La aglutinación bacteriana fue significativamente mayor en los grupos suplementados en todos los tiempos evaluados. Conclusiones. Los resultados demuestran que las SH se comportan como agentes inmunoestimulantes en la fase temprana de la posmuda en gallinas ponedoras Hy Line Brown. Palabras clave: Actividad bactericida, aglutinación, fagocitosis, lisozima (Fuente: DeCS).Rev.MVZ Córdoba 21(1):5198-5210, 2016. ISSN: 0122-0268 ORIGINAL 5199 INTRODUCCIÓNLos mecanismos de la respuesta inmune, innata o adaptativa durante una infección demandan un gasto energético que puede interferir con la capacidad productiva de animales susceptibles. Tradicionalmente, el abordaje de los problemas infecciosos incluye, además, de la disminución de los...

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Studies on wheat bran Arabinoxylan for its immunostimulatory and protective effects against avian coccidiosis

Cited by 73 publications

References 44 publications

The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

Immunological and Therapeutic Evaluation of Wheat (Triticum aestivum) Derived Betaglucans against Coccidiosis in Chicken

Supplementation with humic substances affects the innate immunity in layer hens in posfasting phase

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