Studies with a simplified nitroprusside test for ketone bodies in urine, serum, plasma, and milk

Fraser, J. R. E.; Fetter, Marion C.; Mast, R.L.; Free, Alfred H.

doi:10.1016/0009-8981(65)90228-7

Cited by 12 publications

(6 citation statements)

References 3 publications

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“…The fractions (4 ml each) eluting between a distribution coefficient (Kd) of 0 and 0.85 were neutralized with 0.2 ml of 10 N NaOH, and assayed for somatomedin-C. Small molecular weight somatomedin-C stripped from its binding proteins eluted between Kd 0.27 and 0.52, as described by Zapf et al (13). Serum (16).…”

Section: Methodsmentioning

confidence: 99%

Dietary components that regulate serum somatomedin-C concentrations in humans.

Isley¹,

Underwood²,

Clemmons³

1983

J. Clin. Invest.

419

140

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Section: Methodsmentioning

confidence: 99%

Dietary components that regulate serum somatomedin-C concentrations in humans.

Isley¹,

Underwood²,

Clemmons³

1983

J. Clin. Invest.

419

140

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“…Prior to sorbinil administration and at monthly intervals during treatment, fresh, filtered 24-hour urine samples were analyzed for volume, pH, direct conjugated bilirubin using diazotized 2.4-dichloroaniline; urobilinogen using the Ehrlich test; urine nitrite using the 1.4% p-arsanilic acid and 1.3% 1,2,3.4-tetrahydrobenzo (h) quinolin-3-ol procedure; urine ketones (acetone and acetoacetic acid) using 7.1 % sodium nitroprusside [18,19], Additional 0.5-ml ali quots of the same samples were lyophilized and stored. Total protein analysis was performed on both fresh and lyophilized urine samples using the method ofPesce and Strande [35].…”

Section: Methodsmentioning

confidence: 99%

Reversal of Proteinuria by Sorbinil, an Aldose Reductase Inhibitor in Spontaneously Diabetic (BB) Rats

Beyer‐Mears

Murray²,

Val³

et al. 1988

Pharmacology

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Sorbinil, an aldose reductase inhibitor, was examined as a therapeutic agent to arrest and/or reverse proteinuria in ‘type 1’ insulin-dependent BB rats having spontaneous diabetes mellitus. Prior to sorbinil treatment, diabetic rats exhibited hyperglycemia and increased urinary excretion of urobilinogen, glucose and protein. To assess proteinuria, 24-hour urine samples were analyzed for both total protein and individual components between 30,000 and 100,000 daltons. Daily oral administration of sorbinil (20 mg/kg body weight) was initiated and the aforementioned parameters reevaluated after 1, 2 and 4 months. Results indicated that after 1 month of sorbinil treatment, urobilinogen was normalized in all diabetic BB rats (n = 12), whereas urinary protein excretion was either diminished (67%) or remained constant (16 %), despite persistence of hyperglycemia and glycosuria. These therapeutic effects were sustained after 2 months of sorbinil treatment. After 4 months, protein excretion was normalized (6.56 ± 3.34 mg/24h), despite persistence of hyperglycemia and glycosuria (n = 12); in marked contrast, 6 untreated rats continued to exhibit proteinuria (17.76 ± 2.59 mg/day). Sorbinil diminished albumin and a series of urinary proteins between 30,000 and 100,000 daltons, suggesting that sorbinil may represent a therapeutic approach to manage diabetic nephropathy as indicated by diminution of proteinuria.

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“…Urinary ketones were measured with the nitroprusside reaction (9). The agreement between the Atlas and the nitroprusside method is fair at one level and much better at this level and the levels immediately above or below it.…”

Section: Ketonesmentioning

confidence: 99%

Evaluation of an automated urine chemistry reagent‐strip analyzer

Lott

Johnson

Luke

1995

Clinical Laboratory Analysis

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We evaluated the Miles Inc., Clinitek Atlas Automated Urine Chemistry Analyzer for 11 tests: bilirubin, color, glucose, ketones, leukocyte esterase, nitrite, occult blood, pH, protein, specific gravity, and urobilinogen. The instrument uses a roll of reagent strips affixed to a clear plastic support; urine specimens are automatically pipetted onto these strips. The instrument measures the pads' color using reflectance colorimetry. Specific gravity is measured using a fiberoptic refractive index method. Four hospitals participated in the evaluation, and tests were performed only on fresh urine samples. We found the instrument easy to use; it has walk-away capability with up to 40-specimen loading capacity plus spaces for STATs, calibrators and controls. We found good comparability with chemical tests and other nonreagent strip procedures, as well as good agreement with the Miles Inc. Clinitek 200+ urine chemistry analyzer and visual reading of the Miles Inc. Multistix Reagent Strips. The Clinitek Atlas is rugged and reliable, and is suitable for a high-volume urinalysis laboratory.

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Studies with a simplified nitroprusside test for ketone bodies in urine, serum, plasma, and milk

Cited by 12 publications

References 3 publications

Dietary components that regulate serum somatomedin-C concentrations in humans.

Dietary components that regulate serum somatomedin-C concentrations in humans.

Reversal of Proteinuria by Sorbinil, an Aldose Reductase Inhibitor in Spontaneously Diabetic (BB) Rats

Evaluation of an automated urine chemistry reagent‐strip analyzer

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