Study and modeling of fluctuating dissolved oxygen concentration impact on Corynebacterium glutamicum growth in a scale-down bioreactor

Kaboré, Abdoul Karim; Delaunay, Stéphane; Blanchard, Fabrice; Guédon, Emmanuel; Fick, Michel; Olmos, Éric

doi:10.1016/j.procbio.2018.10.016

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…2). Therefore, microbial cells are exposed to even concentrations of DO 33. Further, localized oxygen depletion is avoided in the bioreactor and this would impact the physiology status of the microbial culture and more biomass is generated as a result 3 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Tracking of Dissolved Oxygen Distribution and Consumption Pattern in a Bespoke Bacterial Growth System

2022

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Microbial cells are used to produce valuable bioproducts. Dense cultures, however, have a high demand for dissolved oxygen which is challenging, considering the low solubility of oxygen in liquid. This study of design and simulation of a gaslift bioreactor highlights a vital limitation of growth, which is the oxygen limitation and sustain aerobic cultures. The aerobic growth has the effect of greatest resource efficiency in that the highest fraction of dosing is done with the largest concentration driving force near critical dissolved oxygen levels and the lowest fraction occurs with near saturated dissolved oxygen values. The results indicate that oxygen homogeneously distributed with limited dead zones at the bottom. The developed system produces 40.5 % more biomass compared with conventional systems, which suffer from oxygen depletion.

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Section: Resultsmentioning

confidence: 99%

Tracking of Dissolved Oxygen Distribution and Consumption Pattern in a Bespoke Bacterial Growth System

2022

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“…The robustness of C. glutamicum is often described together with l ‐lactate and succinate accumulation under oxygen limitation. Interestingly, recent studies have shown robustness even for a C. glutamicum strain deleted for ldhA, which indicates that elevated accumulation of succinate and other acids might compensate the absent l ‐lactate formation. If the NADH/NAD + ratio decreases, substrate uptake and growth under oxygen deprivation keeps rather unchanged or increases .…”

Section: Discussionmentioning

confidence: 99%

Carboxylic acid consumption and production by Corynebacterium glutamicum

Conrady

Lemoine

Limberg

et al. 2019

Biotechnology Progress

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Corynebacterium glutamicum is well-known as an industrial workhorse, most notably for its use in the bulk production of amino acids in the feed and food sector. Previous studies of the effect of gradients in scale-down reactors with complex media disclosed an accumulation of several carboxylic acids and a parallel decrease of growth and product accumulation. This study, therefore, addresses the impact of carboxylic acids, for example, acetate and L-lactate, on the cultivation of the cadaverine producing strain C. glutamicum DM1945Δact3:P tuf -ldcC opt and their potential role in scale up related performance losses. A fluctuating power input in shake flask and stirred tank cultivations with mineral salt was applied to mimic discontinuous oxygen availability.Results demonstrate, whenever sufficient oxygen was available, C. glutamicum recovered from previously occurring stressful conditions like an oxygen limiting phase.Reassimilation of acids was detected simultaneously. In cultures, which were supplemented with either acetate or L-lactate, a rapid cometabolization of both acids in presence of glucose was observed, showing conversion rates of 7.8 and 3.8 mmol g cell dry weight −1 hr −1 , respectively. Uptake of these acids was accompanied by increased oxygen consumption. Proteins related to oxidative stress response, glycogen synthesis, and the main carbon metabolism were found in altered concentrations under oscillatory cultivation conditions. (Proteomics data are available via ProteomeXchange with identifier PXD012760). Virtually no impact on growth or product formation was observed. We conclude that the reduced growth and product formation in scale-down cultivations when complex media was used is not caused by the accumulation of carboxylic acids. K E Y W O R D S carboxylic acid, Corynebacterium glutamicum, heterogeneity, oxygen oscillation, scale down

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“…This approach is useful for optimizing bioprocesses on a lab scale, which can improve large‐scale production by optimizing strains or bioreactor configurations. This scale‐down approach has been applied in multiple bioprocesses with a wide range of microorganisms [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of oxygen concentration on the metabolism of Penicillium chrysogenum

Janoska

Verheijen

Tang

et al. 2022

Engineering in Life Sciences

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In large-scale bioreactors, there is often insufficient mixing and as a consequence, cells experience uneven substrate and oxygen levels that influence product formation. In this study, the influence of dissolved oxygen (DO) gradients on the primary and secondary metabolism of a high producing industrial strain of Penicillium chrysogenum was investigated. Within a wide range of DO concentrations, obtained under chemostat conditions, we observed different responses from P. chrysogenum: (i) no influence on growth or penicillin production (>0.025 mmol L −1 ); (ii) reduced penicillin production, but no growth limitation (0.013-0.025 mmol L −1 ); and (iii) growth and penicillin production limitations (<0.013 mmol L −1 ). In addition, scale down experiments were performed by oscillating the DO concentration in the bioreactor. We found that during DO oscillation, the penicillin production rate decreased below the value observed when a constant DO equal to the average oscillating DO value was used. To understand and predict the influence of oxygen levels on primary metabolism and penicillin production, we developed a black box model that was linked to a detailed kinetic model of the penicillin pathway. The model simulations represented the experimental data during the step experiments; however, during the oscillation experiments the predictions deviated, indicating the involvement of the central metabolism in penicillin production.

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Study and modeling of fluctuating dissolved oxygen concentration impact on Corynebacterium glutamicum growth in a scale-down bioreactor

Cited by 7 publications

References 38 publications

Tracking of Dissolved Oxygen Distribution and Consumption Pattern in a Bespoke Bacterial Growth System

Tracking of Dissolved Oxygen Distribution and Consumption Pattern in a Bespoke Bacterial Growth System

Carboxylic acid consumption and production by Corynebacterium glutamicum

Influence of oxygen concentration on the metabolism of Penicillium chrysogenum

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